##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212937_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31264
Sequences flagged as poor quality	0
Sequence length	56
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.72236438075742	39.0	38.0	40.0	34.0	40.0
2	37.276963920163766	39.0	37.0	40.0	33.0	40.0
3	37.14220829068577	39.0	36.0	40.0	33.0	40.0
4	36.86095829068577	39.0	36.0	39.0	32.0	40.0
5	37.47188459570113	39.0	38.0	40.0	33.0	40.0
6	36.068257420675536	38.0	35.0	39.0	29.0	40.0
7	37.76225051177072	39.0	38.0	40.0	35.0	40.0
8	37.58725690890481	39.0	38.0	40.0	34.0	40.0
9	37.51666453428864	39.0	38.0	40.0	34.0	40.0
10	35.85555271238485	38.0	35.0	39.0	29.0	40.0
11	35.82180783009212	38.0	35.0	39.0	29.0	40.0
12	35.92467374616172	38.0	35.0	39.0	29.0	40.0
13	35.69434493346981	38.0	35.0	39.0	29.0	40.0
14	35.69690378710338	38.0	35.0	39.0	29.0	40.0
15	36.014329580348004	38.0	35.0	39.0	30.0	40.0
16	35.79263689866939	38.0	35.0	40.0	30.0	40.0
17	36.05236054247697	38.0	36.0	40.0	30.0	40.0
18	35.86326125895599	38.0	35.0	40.0	30.0	40.0
19	35.14089687819857	38.0	35.0	39.0	29.0	40.0
20	35.52136642784033	38.0	35.0	40.0	29.0	40.0
21	35.263561924257935	38.0	35.0	39.0	29.0	40.0
22	34.8989252814739	38.0	35.0	39.0	28.0	40.0
23	34.491395854657114	38.0	34.0	39.0	26.0	40.0
24	34.50726074718526	38.0	34.0	39.0	27.0	40.0
25	34.60331371545548	38.0	35.0	39.0	27.0	40.0
26	34.02917093142272	38.0	34.0	39.0	24.0	40.0
27	33.974027635619244	38.0	34.0	39.0	24.0	40.0
28	33.19127430910952	38.0	33.0	39.0	21.0	40.0
29	33.23451893551689	38.0	33.0	39.0	20.0	40.0
30	32.75431806550665	37.0	33.0	39.0	15.0	40.0
31	31.35372952917093	37.0	31.0	39.0	2.0	40.0
32	29.95387666325486	36.0	27.0	39.0	2.0	40.0
33	30.8914406345957	36.0	30.0	39.0	2.0	40.0
34	30.862013817809622	37.0	31.0	39.0	2.0	40.0
35	29.15506653019447	36.0	26.0	39.0	2.0	40.0
36	28.209666069600818	36.0	19.0	39.0	2.0	40.0
37	27.572703428863868	35.0	11.0	39.0	2.0	40.0
38	27.481896110542475	36.0	6.0	39.0	2.0	40.0
39	27.50351842374616	36.0	2.0	39.0	2.0	40.0
40	27.166709314227226	35.0	2.0	39.0	2.0	40.0
41	26.871929375639713	35.0	2.0	39.0	2.0	40.0
42	26.86207778915046	35.0	2.0	39.0	2.0	40.0
43	26.643807574206754	35.0	2.0	39.0	2.0	40.0
44	26.235894319344933	35.0	2.0	39.0	2.0	40.0
45	26.248688587512795	35.0	2.0	39.0	2.0	40.0
46	25.9578428863869	35.0	2.0	39.0	2.0	40.0
47	25.887602354145343	35.0	2.0	39.0	2.0	40.0
48	25.732951637666325	35.0	2.0	39.0	2.0	40.0
49	25.620106192425794	35.0	2.0	39.0	2.0	40.0
50	25.338664278403275	35.0	2.0	39.0	2.0	40.0
51	24.92000383828045	35.0	2.0	39.0	2.0	40.0
52	24.683341862845445	35.0	2.0	39.0	2.0	40.0
53	24.22617067553736	34.0	2.0	39.0	2.0	40.0
54	23.980136898669397	34.0	2.0	39.0	2.0	40.0
55	23.474571392016376	34.0	2.0	39.0	2.0	40.0
56	13.401899948822928	17.0	2.0	22.0	2.0	27.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-1.2757013881806003
1	2	-2.7405891419890267
1	3	-3.4784818398337407
1	4	-3.0605826168158785
1	5	-2.1266748567491405
1	6	-2.400339038319032
1	7	-2.181764160863466
1	8	-2.766077126481676
1	9	-1.8277296082397712
1	10	-1.5290281767636458
1	11	-0.9396697598810988
1	12	-0.43603549133992914
1	13	-0.8746704453612395
1	14	-0.4237105220146802
1	15	0.08890398595759308
1	16	-1.7156495056082335
1	17	-2.446483697488695
1	18	-2.08737994534917
1	19	-1.448632928758009
1	20	-2.05974156581469
1	21	-3.527885310172948
1	22	-2.95956963824597
1	23	-4.494409378382699
1	24	-4.568905067014622
1	25	-3.304602432496729
1	26	-4.324207846048342
1	27	-3.4077009784711976
1	28	-4.246582993879027
1	29	-3.9581909266260205
1	30	-2.537589075539163
1	31	-1.1880063232955749
1	32	-0.5111626501297977
1	33	-0.05775260984088959
1	34	-3.1168883416229143
1	35	-2.040347778169636
1	36	-1.5303753314395934
1	37	-2.314085651835466
1	38	-1.9894981759287056
1	39	-0.9629805838133123
1	40	-3.1074465538814557
1	41	-2.1206226660692167
1	42	-1.7316585771602213
1	43	-1.6006953299606614
1	44	-1.7732444404953185
1	45	-1.101179401504421
1	46	-1.4853746696636918
1	47	-1.0391697282800223
1	48	-1.2174720884970505
1	49	-1.1650392581216238
1	50	-0.39575693776928844
1	51	-1.8429417553816627
1	52	-1.7074213190957899
1	53	-1.7634358324682147
1	54	-1.8260207503043269
1	55	-1.6681095852381986
1	56	-1.0114285961584955
2	1	-1.3962371024663156
2	2	-1.2718391419890267
2	3	-1.9293746969765948
2	4	-2.056118331101594
2	5	-1.8186391424634252
2	6	-3.623553324033317
2	7	-1.1281927322920353
2	8	-1.1187556979102453
2	9	-1.0063010368112018
2	10	-1.0424210339064999
2	11	0.0960445258331859
2	12	-0.26192834848278324
2	13	0.3574724117816146
2	14	-0.06656766487182608
2	15	0.4594397002433084
2	16	0.2888147801060512
2	17	0.3169091596541591
2	18	0.18047719750797597
2	19	-0.2566686430437244
2	20	-0.37224156581469003
2	21	-0.5546710244586635
2	22	0.17435893318260298
2	23	-0.9899450926684139
2	24	-1.2474764955860493
2	25	-0.42960243249672914
2	26	-1.6858149889054843
2	27	-0.9969866927569129
2	28	-2.072475851021885
2	29	-3.663548069483163
2	30	-2.6759819326820207
2	31	-0.6478277518670019
2	32	-0.6763412215583706
2	33	-0.4997168955551743
2	34	-0.31331691305148723
2	35	-0.290347778169636
2	36	-0.7357324742967357
2	37	-0.11765708040689304
2	38	-0.7037838902144209
2	39	-0.7933377266704547
2	40	-0.5494108395957404
2	41	-0.991158380354932
2	42	-1.088801434303079
2	43	-1.0381953299606614
2	44	-0.10806586906674553
2	45	-1.1279651157901363
2	46	-0.003231812520834154
2	47	0.54118741457712
2	48	-0.11925780278276576
2	49	0.10281788473551856
2	50	0.8631716336592845
2	51	0.47848681604691023
2	52	1.1363286809042101
2	53	1.187457024674643
2	54	-0.09834217887575392
2	55	0.4747475576189437
2	56	-0.3417857390156378
3	1	-0.9542728167520309
3	2	-0.3477319991318808
3	3	-0.4784818398337407
3	4	0.04656024032697559
3	5	-0.7695319996062793
3	6	-1.543196181176171
3	7	0.17537869627939529
3	8	0.23392287351832408
3	9	0.27941324890308294
3	10	-0.6718853196207846
3	11	-1.4396697598810988
3	12	-0.7931783484827832
3	13	-1.6246704453612395
3	14	-0.06656766487182608
3	15	-0.6253817283281222
3	16	-0.03707807703680288
3	17	-0.6607694117744103
3	18	-1.1230942310634546
3	19	-1.6629186430437244
3	20	-2.7740272801004053
3	21	-2.3493138816015176
3	22	-2.280998209674543
3	23	-2.172980806954129
3	24	-2.6403336384431917
3	25	-3.590316718211014
3	26	-1.645636417476915
3	27	-2.2291295498997705
3	28	-1.7465829938790272
3	29	-1.4224766409117358
3	30	-0.21616050411059007
3	31	-2.545149180438429
3	32	-1.9397340787012283
3	33	-2.7720383241266013
3	34	-2.50974548448006
3	35	-1.8974906353124936
3	36	0.2910532399889796
3	37	0.7216286338788223
3	38	0.5819303954998638
3	39	0.7155908447581183
3	40	0.8568391604042596
3	41	0.736520191073641
3	42	0.6969128514112057
3	43	0.4707332414679115
3	44	0.6196127023618274
3	45	1.4702491699241484
3	46	0.7289110446220235
3	47	0.2108302717199777
3	48	-0.8960435170684811
3	49	-0.7721821152644814
3	50	-1.7171855091978578
3	51	-2.700084612524517
3	52	-2.171707033381505
3	53	-2.5134358324682147
3	54	-1.3974493217328963
3	55	-0.9538238709524833
3	56	-1.1185714533013513
4	1	-0.49860779212149
4	2	-0.44748569371316194
4	3	-1.0856246969765948
4	4	0.12168339303633502
4	5	-0.033078797635837986
4	6	-1.4188119447229752
4	7	-0.6152616978092738
4	8	-0.41509190480680047
4	9	-0.09250793336292418
4	10	-0.39479172356167425
4	11	-0.4125761638219885
4	12	-0.5173162795172672
4	13	-0.2761482778735598
4	14	0.6058461282316188
4	15	-0.08474133423945318
4	16	1.6304096076922576
4	17	0.5399695044817463
4	18	0.09858064578384074
4	19	1.1905296328183468
4	20	1.138534296254278
4	21	2.042311734162027
4	22	1.3273761745619126
4	23	2.239580769400554
4	24	1.2007993664829186
4	25	1.1781561881929292
4	26	2.4553488041979605
4	27	2.722840893449984
4	28	0.9048948386332896
4	29	-0.7131170350004012
4	30	1.4328542742145345
4	31	-1.7027846484187243
4	32	-5.617073980179061
4	33	-4.007259999003452
4	34	-4.27452380960321
4	35	-7.859313295411017
4	36	-6.983577301882942
4	37	-5.820243287303445
4	38	-7.022749407455802
4	39	-7.235148071498042
4	40	-6.263850494768153
4	41	-5.541804932079071
4	42	-6.269835917061698
4	43	-7.066212571339971
4	44	-6.391471041480539
4	45	-6.643051322686688
4	46	-6.481680088382902
4	47	-6.844588447491844
4	48	-5.836930216575869
4	49	-7.1601131497472394
4	50	-6.445018021513128
4	51	-6.073237321884125
4	52	-6.483283388061306
4	53	-5.699396423601218
4	54	-5.125281834048167
4	55	-5.645942097553469
4	56	-2.464630566601844
5	1	0.8600128975336858
5	2	0.7927441913443047
5	3	1.114375303023408
5	4	1.2917983355650762
5	5	1.320944190869909
5	6	1.2306133426333474
5	7	1.0134739343746304
5	8	1.3124943020897533
5	9	1.4270322965221283
5	10	2.197162299426836
5	11	0.7460445258331845
5	12	0.9068216515172196
5	13	0.3158057451149503
5	14	1.233432335128171
5	15	1.1198563669099784
5	16	2.3533981134393827
5	17	2.0273258263208263
5	18	1.6054771975079731
5	19	2.687081356956277
5	20	2.75692510085198
5	21	3.0078289755413365
5	22	2.995192266515936
5	23	2.724638240664916
5	24	3.5191901710806164
5	25	0.9287309008366051
5	26	2.9162683444278485
5	27	2.211346640576423
5	28	2.5462741489781138
5	29	3.28228526385017
5	30	4.138601400651311
5	31	2.3500889147996666
5	32	2.5507421117749622
5	33	1.13986643777816
5	34	1.8116830869485128
5	35	2.9096522218303633
5	36	1.1934341923699314
5	37	1.1073429195931084
5	38	1.2462161097855784
5	39	2.1774956066628803
5	40	1.6568391604042603
5	41	0.5317582863117352
5	42	1.2611985656969225
5	43	1.1659713367060043
5	44	1.5981841309332552
5	45	1.3512015508765316
5	46	-0.3115651458541677
5	47	-0.42964591875621494
5	48	0.5515755305505685
5	49	-0.1888487819311493
5	50	0.34233830032595236
5	51	1.8118201493802424
5	52	1.6592453475708773
5	53	1.9270403580079751
5	54	1.3620744877909132
5	55	1.1414142242856116
5	56	1.0123809276510283
6	1	0.40816104568182965
6	2	0.4260775246776376
6	3	-0.3448839562358543
6	4	0.41031685408358953
6	5	0.03205530198101769
6	6	1.8787614907814998
6	7	0.6542146751153695
6	8	0.523605413200869
6	9	0.827032296522134
6	10	1.3638289660935001
6	11	0.6053037850924454
6	12	0.4290438737394382
6	13	0.6306205599297598
6	14	-0.27027136857552847
6	15	1.0902267372803465
6	16	-0.49475003470876544
6	17	-0.24674824775324566
6	18	0.41658830861909024
6	19	0.34634061621553514
6	20	0.7272954712223481
6	21	0.5633845310968937
6	22	-0.3788818075581375
6	23	1.3838974999241813
6	24	-0.46969871780827077
6	25	1.3991012712069733
6	26	1.5421942703537752
6	27	2.266902196131973
6	28	3.55368155638552
6	29	2.445248226813135
6	30	2.34600880805872
6	31	2.0315703962811433
6	32	2.4914828525157056
6	33	2.3694960674077876
6	34	2.0894608647262913
6	35	2.4318744440525855
6	36	2.2156564145921536
6	37	2.2666021788523665
6	38	2.8980679616374303
6	39	2.0330511622184346
6	40	2.616098419663519
6	41	2.665091619645068
6	42	1.2908281953265508
6	43	1.9733787441134112
6	44	1.9278137605628842
6	45	2.1474978471728257
6	46	2.140286705997685
6	47	2.566650377540082
6	48	1.6330570120320473
6	49	1.7555956625132971
6	50	1.7682642262518762
6	51	1.922931260491353
6	52	0.755541643867172
6	53	0.3529662839339025
6	54	-1.3934810677646432
6	55	-1.0252524423810563
6	56	0.5494179646880664
7	1	-0.6710215852249348
7	2	-0.13714086612695553
7	3	-0.464935041804182
7	4	-1.0852131586878002
7	5	0.17381775408829725
7	6	0.1673949518287543
7	7	0.07439347460451984
7	8	-0.0012988013585228941
7	9	-0.19595620922499535
7	10	-1.187895171837532
7	11	-0.3436106465806077
7	12	-1.2069714519310608
7	13	-0.7244241399425206
7	14	-1.187257320044239
7	15	-0.08474133423945318
7	16	0.6304096076922576
7	17	-1.3220994610354992
7	18	0.33995995612866636
7	19	0.36294342592179163
7	20	-0.3787070830560708
7	21	0.9733462169206462
7	22	-0.638141066817397
7	23	1.4464773211246893
7	24	0.40769591820705386
7	25	0.10919067095154844
7	26	0.2484522524738253
7	27	1.0331857210361903
7	28	-0.508898264814988
7	29	-0.12691013844867882
7	30	0.12250944662832097
7	31	-1.4614053380738987
7	32	0.9346501577519746
7	33	0.33756758720344493
7	34	0.828924466258858
7	35	0.8648246356234672
7	36	0.4302158015653319
7	37	0.9038946437310393
7	38	1.2875954201304083
7	39	1.9027829629847162
7	40	1.943046056955982
7	41	0.52716058516231
7	42	1.1439571863865758
7	43	1.4510288079703741
7	44	1.7809427516229093
7	45	2.3224659186926218
7	46	-0.37823181252083415
7	47	-0.7066574130090864
7	48	-0.11279228554138498
7	49	0.04678340197689934
7	50	-0.3415697456510607
7	51	0.40952129880552945
7	52	-0.7591454570268255
7	53	-0.3890515960150118
7	54	0.2540285107794169
7	55	0.2161268679637729
7	56	0.6733004678809138
8	1	0.15656462167161322
8	2	0.5869970649075285
8	3	0.36265116509237316
8	4	1.0527178757949613
8	5	0.2772660299503684
8	6	0.7880846070011671
8	7	-0.30491687022306735
8	8	-0.3806091461861101
8	9	-1.161473450604305
8	10	0.19141517299005528
8	11	-1.998783060373711
8	12	-0.9655921415862281
8	13	-1.8278724158045918
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114	51	-1.1511428135827195
114	52	-1.6889028005772708
114	53	-0.6099966790290594
114	54	0.3472596729760973
114	55	0.45622903910042467
114	56	-0.6357672204971188
115	1	0.7600128975336844
115	2	-0.2763034277033114
115	3	0.6643753030234052
115	4	0.7965602403269756
115	5	1.1590394289651442
115	6	0.7425181045381137
115	7	0.5325215534222494
115	8	0.4482085878040394
115	9	0.779413248903083
115	10	-1.9218853196207846
115	11	-2.511098331309668
115	12	-2.0431783484827832
115	13	-2.9818133025041007
115	14	-2.4951390934432567
115	15	-2.4825245854709763
115	16	-2.644220934179664
115	17	-2.4107694117744103
115	18	-2.1230942310634546
115	19	-2.484347214472294
115	20	-2.452598708671836
115	21	-1.8135995958872329
115	22	-2.1024267811031123
115	23	-1.208695092668414
115	24	-2.6760479241574764
115	25	-2.0188881467824444
115	26	-1.395636417476915
115	27	-1.0505581213283435
115	28	-1.2465829938790272
115	29	-1.4939052123403052
115	30	-2.3947319326820207
115	31	-2.5094348947241443
115	32	-2.3683055072726553
115	33	-1.629181181269459
115	34	-1.4383169130514872
115	35	-2.1832049210267783
115	36	-1.637518188582451
115	37	-1.9569427946926083
115	38	-2.3109267473572785
115	39	-1.07012344095617
115	40	-1.0360179824528828
115	41	-1.0491940946406473
115	42	-1.3030871485887943
115	43	-2.3506953299606614
115	44	-1.0946730119238879
115	45	-1.4940365443615633
115	46	-1.0925175268065495
115	47	-0.7534554425657376
115	48	-1.8246149456399081
115	49	-0.9864678295501967
115	50	-0.3243283663407155
115	51	0.19277253033262554
115	52	0.18543582376135248
115	53	-0.22772154675392997
115	54	-0.004592178875753916
115	55	1.5818904147618014
115	56	0.23857140384150455
116	1	0.1528700403908303
116	2	0.6522680008681192
116	3	0.7000895887376899
116	4	0.7965602403269756
116	5	-0.09096057103485577
116	6	0.20680381882382903
116	7	0.5682358391365341
116	8	0.8410657306611853
116	9	0.9936989631887982
116	10	-0.20759960533506927
116	11	0.1317588115474706
116	12	-0.7931783484827832
116	13	-0.696099016789816
116	14	0.290575192271028
116	15	0.05318970024330838
116	16	0.6057790658203359
116	17	0.053516302511305014
116	18	0.16262005465083007
116	19	0.9799384998134215
116	20	0.47597271989959467
116	21	1.4721146898270518
116	22	0.11185893318260298
116	23	0.684162050188732
116	24	0.966809218699666
116	25	1.0168261389318403
116	26	-1.002779274619769
116	27	0.770870450100233
116	28	-0.10372585102188481
116	29	-0.2796194980545934
116	30	0.8552680673179793
116	31	0.2762793909901404
116	32	1.2388373498702023
116	33	1.4422473901591104
116	34	2.2045402298056587
116	35	1.3525093646875064
116	36	3.8267675257032643
116	37	4.043057205307392
116	38	4.081930395499864
116	39	4.358447987615261
116	40	5.071124874689975
116	41	3.736520191073641
116	42	3.589769994268348
116	43	4.363590384325054
116	44	4.905326988076112
116	45	4.791677741352721
116	46	4.586053901764881
116	47	3.9965445574342624
116	48	4.996813625788661
116	49	4.906389313306946
116	50	4.282814490802142
116	51	1.1927725303326255
116	52	2.4354358237613525
116	53	2.3794213103889277
116	54	2.673979249695673
116	55	1.867604700476086
116	56	2.3099999752700757
117	1	0.4742986118193997
117	2	-0.4191605705604573
117	3	-1.0141961255480254
117	4	-0.9891540453873091
117	5	-1.2695319996062793
117	6	-2.0789104668904628
117	7	-1.431764160863466
117	8	-1.0517914121959606
117	9	-1.5063010368112018
117	10	-0.6004567481922152
117	11	-2.54681261702396
117	12	-2.0074640627684985
117	13	-2.446099016789816
117	14	-1.3522819505861108
117	15	-1.0539531568995457
117	16	-0.03707807703680288
117	17	0.053516302511305014
117	18	0.2697629117936913
117	19	-0.9129186430437244
117	20	-0.9883129943861206
117	21	0.18640040411276715
117	22	-0.5667124953888276
117	23	0.3984477644744402
117	24	0.35966636155680476
117	25	-0.5188881467824444
117	26	0.2829350110945157
117	27	-1.479129549899767
117	28	-0.9251544224504578
117	29	-0.6367623551974475
117	30	-0.0018747898248747674
117	31	0.3477079624187134
117	32	-0.5468769358440859
117	33	-0.9863240384123166
117	34	-0.11688834162291428
117	35	0.10250936468750638
117	36	1.5410532399889796
117	37	0.4002000624502493
117	38	-1.2752124616429938
117	39	-1.8915520123847394
117	40	-2.678875125310025
117	41	-2.9420512374977896
117	42	-1.9816585771602213
117	43	-0.9578381871035191
117	44	-3.4518158690667455
117	45	-1.7083222586472786
117	46	-0.6996603839494071
117	47	-0.36059829970859525
117	48	-2.0389006599256234
117	49	-1.7721821152644814
117	50	-1.3243283663407155
117	51	-0.8429417553816627
117	52	-1.3502784619529322
117	53	-3.0134358324682147
117	54	-1.2188778931614692
117	55	-0.4895381566667716
117	56	-1.5828571675870666
118	1	-0.18826296453528357
118	2	-0.5509339695752331
118	3	0.2247201306096116
118	4	0.6734075309673742
118	5	0.44967982305381327
118	6	-0.03950159989538804
118	7	-0.029054801257551333
118	8	-0.10474707722058696
118	9	-0.9200941402594793
118	10	-0.08444689597546784
118	11	-2.722920991408195
118	12	-1.7586955898620928
118	13	-2.827872415804592
118	14	-3.187257320044239
118	15	-2.4985344376877237
118	16	-2.438555909549123
118	17	-1.5634787713803249
118	18	-3.073833147319611
118	19	-1.3611945051126924
118	20	-2.689051910642277
118	21	-1.4404468865276314
118	22	-1.7071065840587778
118	23	-1.863867506461517
118	24	-1.7992006335170814
118	25	-1.0632231221519035
118	26	-2.2343063682158295
118	27	-2.3806073824120837
118	28	-2.37096723033223
118	29	-2.299323931552127
118	30	-2.2223181395785723
118	31	-1.1510605104876923
118	32	0.31396050257956176
118	33	-0.007259999003451867
118	34	-0.6538341544307968
118	35	0.7958591183820865
118	36	-2.5008186811932873
118	37	-3.8892088045448254
118	38	-4.19516320055925
118	39	-4.40756186460149
118	40	-3.7810918740784984
118	41	-3.47283941483769
118	42	-2.9594910894754918
118	43	-3.2386263644434194
118	44	-2.736298627687436
118	45	-2.9533961502728943
118	46	-3.0334042263139374
118	47	-2.8445884474918444
118	48	-2.561068147610353
118	49	-2.091147632505862
118	50	-3.4450180215131283
118	51	-3.969789046022054
118	52	-3.483283388061306
118	53	-4.285603320152944
118	54	-2.9183852823240315
118	55	-3.6804248561741595
118	56	-1.740492635567362
119	1	-0.5675733093628708
119	2	-1.0681753488855819
119	3	-0.8097626280110788
119	4	-1.0852131586878002
119	5	-1.7227339700496316
119	6	-1.763639530929872
119	7	-1.2014685943610033
119	8	-1.3461263875654197
119	9	-1.885611381638789
119	10	-2.0499641373547774
119	11	-0.10223133623578207
119	12	-1.3104197277931249
119	13	-0.13821724339079822
119	14	-1.014843526940794
119	15	-2.533017196308414
119	16	-1.507521426790504
119	17	-2.666927047242396
119	18	-2.039350388698921
119	19	-2.809470367181653
119	20	-1.8269829451250317
119	21	-1.130102058941425
119	22	-1.2933134806105002
119	23	-0.2086950926684139
119	24	-0.7302351162757077
119	25	-0.5459817428415548
119	26	-1.0963753337330715
119	27	-0.725434968618984
119	28	-3.129587919987401
119	29	-1.368289448793508
119	30	-1.705076760268227
119	31	-1.6338191311773471
119	32	-1.0653498422480254
119	33	-0.7658806886586227
119	34	-0.6883169130514872
119	35	-1.8593132954110168
119	36	-2.2939221294691485
119	37	-1.6478294941999962
119	38	-2.0227494074558017
119	39	-1.028251519773903
119	40	-0.9190229085612565
119	41	-1.9900807941480352
119	42	-2.718111779130666
119	43	-4.480005674788249
119	44	-2.736298627687436
119	45	-2.8154651157901363
119	46	-2.65409388148635
119	47	-2.5687263785263283
119	48	-2.6645164234724206
119	49	-2.19459590836793
119	50	-3.3415697456510607
119	51	-3.9353062874013673
119	52	-4.345352353578548
119	53	-3.630430906359841
119	54	-3.435626661634373
119	55	-1.8873214078982983
119	56	-3.809458152808741
120	1	-0.11929744729390279
120	2	0.1042384442178701
120	3	0.36265116509237316
120	4	-0.36107522765331623
120	5	-0.3434236252220444
120	6	0.1329121932080639
120	7	-0.9256065253954802
120	8	-1.2081953530826581
120	9	-1.3683700023284402
120	10	-1.8775503442513255
120	11	-0.5849899569254333
120	12	-0.6897300726207121
120	13	0.24109310143678897
120	14	-0.4631193890097549
120	15	-0.7743965066532468
120	16	-0.40407315092843277
120	17	0.8158315734472623
120	18	1.2365116802665952
120	19	0.05259859833558522
120	20	0.966120503150826
120	21	1.5595531134723686
120	22	0.913583071113635
120	23	1.5844083556074509
120	24	1.9939028147587763
120	25	1.247121705434303
120	26	0.07603845937038045
120	27	0.7573236520706743
120	28	-0.5433810234356784
120	29	0.8730898615513212
120	30	-0.8774905533716755
120	31	-2.0131294760049343
120	32	-2.892936049144577
120	33	-1.9038117231413807
120	34	-2.5848686371894196
120	35	-2.583451226445497
120	36	1.395733042944645
120	37	1.283204988558623
120	38	1.9772505925441983
120	39	1.5234726181571325
120	40	1.7706322638525371
120	41	1.1133674817140324
120	42	1.385336496731405
120	43	2.002752945901406
120	44	1.5050806826573933
120	45	1.3569486773133121
120	46	0.41487163575502706
120	47	0.01748051802539763
120	48	-1.2507233200241465
120	49	0.04678340197689934
120	50	-0.8932938835820963
120	51	0.06469371259863266
120	52	-0.1384558018544091
120	53	0.7143966798470558
120	54	-1.8149370064619603
120	55	-1.3355972699672627
120	56	0.7767487437429832
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66.0
3	11.0
4	36.0
5	15.0
6	78.0
7	194.0
8	120.0
9	132.0
10	238.0
11	176.0
12	200.0
13	270.0
14	257.0
15	230.0
16	280.0
17	313.0
18	404.0
19	506.0
20	664.0
21	686.0
22	779.0
23	979.0
24	1160.0
25	698.0
26	484.0
27	426.0
28	473.0
29	515.0
30	635.0
31	851.0
32	985.0
33	1234.0
34	1607.0
35	1838.0
36	2483.0
37	3960.0
38	4454.0
39	2827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	41.111182190378706	44.01228249744115	5.824590583418629	9.051944728761514
11	35.96148925281474	38.53313715455476	13.897773797338791	11.607599795291708
12	60.71839815762539	18.09109518935517	11.741939611054248	9.4485670419652
13	18.267016376663257	32.57740532241556	18.107088024564995	31.048490276356194
14	19.242579324462643	49.414662231320364	19.639201637666325	11.703556806550665
15	38.87538382804504	14.713408393039916	35.30258444216991	11.108623336745138
16	14.831755373592632	15.484263050153531	47.8217758444217	21.86220573183214
17	34.58930399181167	15.567425793244627	13.846596724667348	35.99667349027635
18	33.52098259979529	19.041069600818833	20.880245649948822	26.557702149437052
19	19.213792221084955	17.684877175025587	49.13318833162743	13.968142272262027
20	28.246545547594675	32.523029682702145	21.27047082906858	17.959953940634595
21	27.50767656090072	17.00997952917093	32.17118730808598	23.311156601842377
22	26.720829068577277	22.050921187308088	14.930910951893553	36.29733879222108
23	24.967211541537377	43.82457375003999	15.642493842167557	15.565720866255079
24	16.447031729785056	40.59301432958035	23.403915046059364	19.55603889457523
25	18.274030462050426	22.71214642262895	29.31332394726737	29.700499168053245
26	40.947378535054284	19.110911827819237	21.37206546456138	18.5696441725651
27	21.752362646163704	16.898926798013775	25.88819477815153	35.46051577767099
28	17.251321480057666	35.345186609002084	29.649207111965403	17.75428479897485
29	30.241276554839953	17.520587010157325	40.60687622160275	11.631260213399981
30	18.83156443389874	32.8277808061051	37.28476608843428	11.055888671561869
31	34.18951418951419	17.92528459195126	38.54096520763187	9.344236010902678
32	21.000128303823455	15.730048755452913	52.43777264562483	10.832050295098794
33	24.286080985689534	12.690111018417507	43.71751267406789	19.306295321825065
34	17.87410164271047	13.732032854209445	45.796971252566735	22.596894250513348
35	16.22107391597394	26.626440286292002	41.94883974708733	15.203646050646725
36	23.907108213150032	18.183278193556678	37.78948382744997	20.120129765843316
37	10.705455479562021	14.91506919693029	53.50480043669524	20.874674886812446
38	16.150805129688557	15.495130652781796	51.014045575804325	17.340018641725326
39	14.144271570014144	19.711971197119713	43.34897775491835	22.794779477947795
40	15.274739415776606	16.25916870415648	49.86809934371381	18.597992536353107
41	20.68410721755639	14.303182417865301	51.43031824178653	13.582392122791775
42	27.32305513534391	13.447487849625029	48.68840323151694	10.541053783514114
43	24.282908927019285	15.00498985931816	48.69136915301162	12.020732060650936
44	16.394128629369728	21.14208459409	51.10088199317582	11.36290478336445
45	26.617083615055215	19.70121381886088	43.27892076370779	10.402781802376122
46	16.465966900637515	22.693025951445684	43.531457273488314	17.30954987442849
47	20.91966616182773	20.91322140946734	44.32056198240583	13.8465504462991
48	19.72852721176167	21.15359814289399	44.79623420170235	14.321640443641993
49	18.862150059598594	22.341419413034373	45.74917045198286	13.04726007538417
50	17.327530625402964	21.795615731785944	47.07607994842037	13.800773694390717
51	19.535303406271147	22.345396538944925	44.197737746124844	13.921562308659082
52	19.783940664301838	20.564334085778782	45.720735246694616	13.930990003224766
53	13.270768833849328	22.997291021671824	46.81050051599588	16.921439628482972
54	16.540892672858618	25.493421052631575	43.89512383900929	14.070562435500516
55	20.32711787857281	19.936770114200915	43.554422865991356	16.181689141234916
56	16.711825043545577	25.51770853493323	43.66169924520998	14.108767176311204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	9.5
14	19.0
15	24.5
16	30.0
17	36.0
18	42.0
19	80.0
20	118.0
21	259.0
22	475.5
23	551.0
24	522.5
25	494.0
26	558.0
27	622.0
28	760.5
29	1094.0
30	1289.0
31	1405.5
32	1522.0
33	1646.5
34	1771.0
35	1768.0
36	1765.0
37	1433.0
38	1710.0
39	2319.0
40	1460.0
41	601.0
42	513.5
43	426.0
44	380.5
45	335.0
46	347.0
47	618.0
48	877.0
49	1036.5
50	1196.0
51	904.0
52	612.0
53	587.5
54	818.0
55	1073.0
56	1718.0
57	2363.0
58	1995.5
59	1628.0
60	1403.0
61	1178.0
62	1179.0
63	1093.0
64	1006.0
65	902.0
66	798.0
67	717.5
68	637.0
69	752.0
70	867.0
71	935.0
72	978.0
73	953.0
74	698.0
75	443.0
76	292.0
77	141.0
78	95.5
79	34.0
80	18.0
81	13.0
82	8.0
83	6.0
84	4.0
85	3.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.009595701125895598
24	0.0
25	0.03838280450358239
26	0.13114124872057317
27	0.15672978505629478
28	0.15672978505629478
29	0.17592118730808598
30	0.24628966223132037
31	0.25268679631525076
32	0.28147389969293757
33	0.31345957011258957
34	0.3070624360286591
35	0.3422466734902764
36	0.41901228249744116
37	0.38702661207778916
38	0.48298362333674516
39	0.49897645854657113
40	0.5757420675537359
41	0.5981320368474924
42	0.6237205731832139
43	0.6429119754350051
44	0.6333162743091095
45	0.6557062436028659
46	0.6589048106448311
47	0.7388689866939611
48	0.7932446264073695
49	0.7132804503582395
50	0.7804503582395087
51	0.7452661207778914
52	0.8124360286591606
53	0.8188331627430911
54	0.8188331627430911
55	0.850818833162743
56	0.8380245649948823
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	31264.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.618219037871036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5	37.67911975435005
2	10.875	10.57446264073695
3	4.440789473684211	6.477098259979528
4	2.2171052631578947	4.311668372569089
5	1.2894736842105263	3.134595701125895
6	0.9013157894736843	2.629222108495394
7	0.625	2.1270470829068575
8	0.35526315789473684	1.3817809621289663
9	0.2828947368421053	1.2378454452405323
>10	1.342105263157895	10.155450358239507
>50	0.08552631578947369	2.9106960081883315
>100	0.06578947368421052	6.678607983623337
>500	0.006578947368421052	2.258188331627431
>1k	0.013157894736842105	8.444216990788126
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1589	5.082523029682702	No Hit
GGGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1051	3.361693961105425	No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	706	2.258188331627431	No Hit
GGGCGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	370	1.1834698055271238	No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	295	0.9435772773797338	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	257	0.8220317297850563	No Hit
GGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	222	0.7100818833162743	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	219	0.7004861821903787	No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	210	0.671699078812692	No Hit
GGGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	190	0.607727737973388	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT	118	0.3774309109518936	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGG	106	0.3390481064483111	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC	101	0.32305527123848515	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC	95	0.303863868986694	No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92	0.29426816786079835	No Hit
GGGCGAGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC	91	0.29106960081883315	No Hit
GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.26228249744114635	No Hit
GGGCGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.22389969293756398	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCAGGCCGCCCC	69	0.2207011258955988	No Hit
GGGCGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	66	0.21110542476970315	No Hit
GGGCGAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG	62	0.19831115660184237	No Hit
GGGCGAGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG	61	0.19511258955987718	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGCC	59	0.1887154554759468	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	55	0.17592118730808598	Illumina Single End Adapter 2 (95% over 23bp)
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCAGGCCGCCCC	55	0.17592118730808598	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAGCAGT	53	0.16952405322415556	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCT	50	0.15992835209825998	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCCGGCCGCC	49	0.15672978505629478	No Hit
GGGCGAGGGCTGGGCGAGGGGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCG	46	0.14713408393039917	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	45	0.14393551688843398	Illumina Single End Adapter 2 (96% over 30bp)
GGGCGAGGGGGCTGGGCGAGGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCG	44	0.14073694984646878	No Hit
GGGCGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCAGGCCGCTCC	42	0.1343398157625384	No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGA	40	0.127942681678608	No Hit
GGGCGAGGGAAGAATGCACAAAGCAGAATCACATTCTTCCTTTCAAATTTCTCCTT	36	0.11514841351074719	No Hit
GGGCGAGGGCAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.11514841351074719	No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.11194984646878199	No Hit
GGGCGAGGGCAGTCCGCAGAGTCGACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.10555271238485157	No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	32	0.1023541453428864	Illumina Single End Adapter 2 (100% over 29bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.031985670419651994	0.0	0.0	0.0	0.0
11	0.13114124872057317	0.0	0.0	0.0	0.0
12	0.13114124872057317	0.0	0.0	0.0	0.0
13	0.1343398157625384	0.0	0.0	0.0	0.0
14	0.17272262026612079	0.0	0.0	0.0	0.0
15	0.17272262026612079	0.0	0.0	0.0	0.0
16	0.17592118730808598	0.0	0.0	0.0	0.0
17	0.17911975435005117	0.0	0.0	0.0	0.0
18	0.18231832139201637	0.0	0.0	0.0	0.0
19	0.18231832139201637	0.0	0.0	0.0	0.0
20	0.2047082906857728	0.0	0.0	0.0	0.0
21	0.20790685772773798	0.0	0.0	0.0	0.0
22	0.22070112589559876	0.0	0.0	0.0	0.0
23	0.22709825997952918	0.0	0.0	0.0	0.0
24	0.23989252814738998	0.0	0.0	0.0	0.0
25	0.24628966223132037	0.0	0.0	0.0	0.0
26	0.25268679631525076	0.0	0.0	0.0	0.0
27	0.25268679631525076	0.0	0.0	0.0	0.0
28	0.39342374616171955	0.0	0.0	0.0	0.0
29	0.39982088024564993	0.0	0.0	0.0	0.0
30	0.44779938587512796	0.0	0.0	0.0	0.0
31	0.44779938587512796	0.0	0.0	0.0	0.0
32	0.44779938587512796	0.0	0.0	0.0	0.0
33	0.44779938587512796	0.0	0.0	0.0	0.0
34	0.4733879222108495	0.0	0.0	0.0	0.0
35	0.5789406345957011	0.0	0.0	0.0	0.0
36	0.5853377686796315	0.0	0.0	0.0	0.0
37	0.5885363357215967	0.0	0.0	0.0	0.0
38	0.5917349027635619	0.0	0.0	0.0	0.0
39	0.5949334698055271	0.0	0.0	0.0	0.0
40	0.5981320368474923	0.0	0.0	0.0	0.0
41	0.6045291709314228	0.0	0.0	0.0	0.0
42	0.6173234390992836	0.0	0.0	0.0	0.0
43	0.6237205731832139	0.0	0.0	0.0	0.0
44	0.6301177072671443	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACAAG	15	0.008269122	50.130226	46
ATCCTAC	15	0.008269122	50.130226	43
CGGAGCA	25	2.0093925E-5	50.130226	41
CCTACAA	15	0.008269122	50.130226	45
TCCTACA	15	0.008269122	50.130226	44
GGCGCGG	25	2.0093925E-5	50.130226	42
CCCGGCA	15	0.008269122	50.130226	37
CATCCTA	15	0.008269122	50.130226	42
CGCCACC	25	2.0093925E-5	50.130226	35
CATTTGG	15	0.008269122	50.130226	45
GTGGGGG	15	0.008269122	50.130226	36
TACAAGG	15	0.008269122	50.130226	47
TCGCCAC	25	2.0093925E-5	50.130226	34
TCAGAGG	15	0.008269122	50.130226	48
CGGTGGG	15	0.008269122	50.130226	34
CCTTTGG	15	0.008269122	50.130226	45
GATCATC	15	0.008269122	50.130226	39
GGTAGGC	15	0.008269122	50.130226	48
ATTTGGT	15	0.008269122	50.130226	46
ATCATCC	15	0.008269122	50.130226	40
>>END_MODULE