FastQCFastQC Report
Tue 31 May 2016
SRR212936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343729
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA27790.8084857547661093No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA17880.5201772326454852No Hit
TCACTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC14400.4189346840097868No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA13600.39566053489813197No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG12360.359585603775067No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA11370.3307838442493941No Hit
TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10760.31303730555175735No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9330.27143476401467437No Hit
TCACTTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG9110.26503437300896926No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA7350.21383124496332867No Hit
TCACTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT7310.21266753750774592No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA6120.17804724070415937No Hit
TCACTTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT6000.17455611833741116No Hit
TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5650.16437367810106215No Hit
TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5320.15477309159250457No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA5320.15477309159250457No Hit
TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5130.14924548117848654No Hit
TCACTTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5070.14749991999511242No Hit
TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5010.14575435881173832No Hit
TCACTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4750.1381902603504505No Hit
TCACTTGGGGAACTTTGTCTGCTGGTGCCCTGTGAGGGTTTTGCATAAATTCTCCCTA4740.1378993334865548No Hit
TCACTTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4390.12771689325020583No Hit
TCACTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA4310.12538947833904035No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA4150.12073464851670937No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAA4130.12015279478891801No Hit
TCACTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3990.11607981869437842No Hit
TCACTTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG3850.11200684259983884No Hit
TCACTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3830.11142498887204745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA307.9021993E-752.07258652
GTGAATC150.007176154852.07258652
ACGAACA150.007176154852.07258652
TAGACGT150.007184431452.0574149
AACGTAT150.007188572552.04982845
CCTAATA150.007188572552.04982843
CTACGAT150.007188572552.04982843
CTACGTA150.007205154352.0195139
TACGTAC150.007205154352.0195140
TGCGATC459.276846E-1151.996832
CGATATA150.00721760951.99679632
ACGATAT150.007225921451.98166731
TACGATA150.007225921451.98166730
TCGTAAC150.007225921451.98166728
CGTAACC150.007225921451.98166729
TTACGAT150.007225921451.98166729
TCGGTTA150.007225921451.98166730
AAGCGAT150.007230079751.97410610
CACTAAT150.007230079751.97410612
TCATCGG150.007230079751.97410627
Produced by FastQC (version 0.11.5)