##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212936_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 343729 Sequences flagged as poor quality 0 Sequence length 58 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.13197897180628 39.0 38.0 40.0 35.0 40.0 2 37.43454291025779 39.0 36.0 40.0 33.0 40.0 3 37.32893936793229 39.0 36.0 40.0 33.0 40.0 4 37.475002109219766 39.0 37.0 40.0 33.0 40.0 5 37.33915962866095 39.0 36.0 40.0 33.0 40.0 6 37.89426554058575 39.0 38.0 40.0 35.0 40.0 7 36.985631122192196 39.0 35.0 40.0 32.0 40.0 8 37.8453316420785 39.0 38.0 40.0 35.0 40.0 9 37.78978206668626 39.0 38.0 40.0 35.0 40.0 10 36.75692187740924 38.0 35.0 39.0 31.0 40.0 11 37.03344495227345 39.0 36.0 39.0 33.0 40.0 12 36.788781278274456 38.0 35.0 39.0 31.0 40.0 13 36.677757768474585 38.0 35.0 39.0 31.0 40.0 14 36.68961885671561 38.0 35.0 39.0 31.0 40.0 15 36.655978983443354 38.0 35.0 39.0 31.0 40.0 16 36.239941930997965 38.0 35.0 39.0 30.0 40.0 17 35.88923832437764 38.0 35.0 39.0 29.0 40.0 18 35.872702041433804 38.0 35.0 39.0 29.0 40.0 19 35.735844226119994 38.0 35.0 39.0 29.0 40.0 20 35.676102394619015 38.0 35.0 39.0 29.0 40.0 21 35.495349534080624 38.0 35.0 39.0 28.0 40.0 22 35.37672410532716 38.0 34.0 39.0 28.0 40.0 23 35.2702419638727 38.0 33.0 39.0 28.0 40.0 24 35.26693121616157 38.0 33.0 39.0 28.0 40.0 25 35.13880120676463 38.0 33.0 39.0 28.0 40.0 26 34.72029418524477 38.0 33.0 39.0 26.0 40.0 27 34.63732475293036 38.0 33.0 39.0 26.0 40.0 28 34.2931757285536 38.0 33.0 39.0 26.0 40.0 29 34.26537475743973 38.0 33.0 39.0 26.0 40.0 30 33.88975908346401 37.0 33.0 39.0 25.0 40.0 31 33.4009903150447 37.0 32.0 39.0 23.0 40.0 32 33.296812314352294 37.0 32.0 38.0 23.0 40.0 33 33.06874310866991 36.0 31.0 38.0 22.0 40.0 34 32.559018878244196 36.0 31.0 38.0 20.0 40.0 35 32.52476805855776 36.0 31.0 38.0 20.0 40.0 36 33.542130573795056 38.0 33.0 39.0 23.0 40.0 37 33.438263864847016 38.0 33.0 39.0 23.0 40.0 38 33.16708220720393 37.0 32.0 39.0 22.0 40.0 39 33.14594346127327 37.0 32.0 39.0 22.0 40.0 40 32.97760154074867 37.0 32.0 39.0 22.0 40.0 41 33.185142946914574 38.0 33.0 39.0 21.0 40.0 42 33.08913999109764 38.0 32.0 39.0 21.0 40.0 43 33.01173308042091 37.0 32.0 39.0 20.0 40.0 44 32.86884144194991 37.0 32.0 39.0 20.0 40.0 45 32.588225026110685 37.0 32.0 39.0 18.0 40.0 46 32.171597392131595 36.0 31.0 39.0 14.0 40.0 47 32.09852820099555 36.0 31.0 39.0 15.0 40.0 48 31.876745924841956 36.0 31.0 39.0 13.0 40.0 49 31.78215105504627 36.0 30.0 39.0 11.0 40.0 50 31.500621128854416 36.0 30.0 39.0 2.0 40.0 51 31.22199465276424 36.0 30.0 39.0 2.0 40.0 52 31.173354590389522 36.0 30.0 39.0 2.0 40.0 53 30.96185076033736 36.0 30.0 39.0 2.0 40.0 54 30.809969481771976 36.0 30.0 39.0 2.0 40.0 55 30.62354645665626 36.0 30.0 39.0 2.0 40.0 56 30.337672992386445 36.0 30.0 39.0 2.0 40.0 57 29.946460729237277 35.0 29.0 39.0 2.0 40.0 58 29.62656336823486 35.0 29.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -1.7661634021922268 2 2 -2.45194897884096 2 3 -0.8264226792185667 2 4 -8.72007593944501 2 5 -1.9771959707198121 2 6 -1.12260065638025 2 7 -0.9304150000510347 2 8 -1.1407007247024907 2 9 -1.1215109311604223 2 10 -4.366327736981752 2 11 -7.53092182053679 2 12 -7.663339011871081 2 13 -10.812059400889638 2 14 -2.9841332184445193 2 15 -11.900625486214274 2 16 -13.81576489916975 2 17 -8.514871295769986 2 18 -11.843626591130125 2 19 -6.186109166887292 2 20 -17.15615999509903 2 21 -6.2178262051128215 2 22 2.2825635122995465 2 23 -5.671071675398991 2 24 1.110694531443567 2 25 -4.1835769356491355 2 26 1.1884627801569572 2 27 -3.8000223727877653 2 28 2.6666273893063135 2 29 -4.092568467660019 2 30 0.40411434667935 2 31 5.148459518913498 2 32 -6.972178939919452 2 33 -6.0409194076079835 2 34 0.9607727118356983 2 35 2.3592337757016395 2 36 -6.116692153234368 2 37 -4.3251508163792565 2 38 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-6.4638472139114995 119 58 -8.15092933722384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 183.0 3 23.0 4 122.0 5 130.0 6 215.0 7 396.0 8 328.0 9 597.0 10 743.0 11 800.0 12 1055.0 13 1220.0 14 1425.0 15 1537.0 16 1751.0 17 1888.0 18 2021.0 19 2080.0 20 2210.0 21 2187.0 22 2659.0 23 2803.0 24 3286.0 25 3988.0 26 4996.0 27 6148.0 28 7192.0 29 8664.0 30 10021.0 31 11374.0 32 13486.0 33 16319.0 34 19844.0 35 24970.0 36 33073.0 37 45947.0 38 53350.0 39 54510.0 40 188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 49.5785924376471 36.263742657733275 3.6618964358549904 10.495768468764636 11 33.12900569925726 22.004253350750155 22.854923500781137 22.011817449211442 12 34.92897020617987 13.894085165930136 26.503728227760824 24.67321640012917 13 21.853844162116083 14.853852889922003 31.82478056841291 31.467522379549006 14 20.49899775695388 19.973001987030482 37.02538918741218 22.502611068603464 15 29.224185332049373 19.123786471318976 30.80915488655307 20.84287331007858 16 32.466274303302896 20.690427633397242 26.1615982358195 20.681699827480372 17 31.595423111946396 21.318953704538774 24.315808766841126 22.769814416673697 18 30.738258157612997 21.850707536191408 24.4865125913513 22.924521714844296 19 26.834398629128685 26.33661407586923 24.467085414708936 22.361901880293143 20 28.200204813108037 26.420018153889124 24.250570218312152 21.129206814690686 21 26.877578451852415 24.376661992237732 27.320679402061014 21.42508015384884 22 25.364458628745318 21.588227935379326 29.54973249274865 23.49758094312671 23 30.071278947980918 22.678342837193064 26.018270685441642 21.232107529384383 24 31.6702984036843 22.95587512255294 22.368784711211447 23.00504176255131 25 26.957283208574196 21.763365907444527 21.19082183929782 30.088529044683458 26 25.151685344212964 22.594350466623403 25.434831320077173 26.819132869086452 27 22.45575446528584 21.39118493367898 24.129854789108165 32.02320581192702 28 24.976865458820516 27.448332858032487 21.496208263250708 26.07859341989629 29 31.002636219207737 22.93714392127423 21.769176603409043 24.29104325610899 30 27.10235757471669 22.547892329949846 25.31882129997002 25.030928795363444 31 33.39979914710292 20.881424017931216 23.345510646659 22.373266188306868 32 35.394102001501565 20.095622770208532 24.10240890228785 20.40786632600206 33 33.61306636481881 20.05348293812798 26.2671644440306 20.0662862530226 34 26.381573203736576 23.052993044844747 30.642842592323138 19.92259115909554 35 24.549042205807883 31.86797515730409 25.666323246081223 17.916659390806807 36 26.523108632113374 33.10315916950988 22.645178569349188 17.728553629027562 37 30.676607674683325 28.970313805521506 21.711650601322297 18.641427918472868 38 28.361681619768962 29.922511771746063 21.455299396925515 20.26050721155946 39 29.636886634796227 26.55368383050828 21.41879734710633 22.390632187589162 40 25.88387036665007 25.445392635553965 22.445925447139558 26.22481155065641 41 22.586928797293286 28.805154873311945 23.566133437378074 25.041782892016702 42 22.262478229719417 25.879985321442927 23.25706696800424 28.600469480833418 43 23.713772284177324 22.54673815855005 24.801004226020755 28.938485331251872 44 24.578736340050842 22.436239011620966 23.740431120079432 29.244593528248757 45 27.403119622407473 24.16498999822385 24.29135887304587 24.140531506322812 46 23.549572530109256 27.665862976681343 25.21984997786941 23.564714515339997 47 24.008215107641213 23.875957700935125 26.584030063798174 25.531797127625484 48 25.869206142050206 22.32829264171665 27.897038217395487 23.90546299883766 49 28.218568980990455 22.58272240935209 25.217077122110982 23.981631487546476 50 21.6623782312608 22.817778891573006 28.327277071780543 27.192565805385655 51 20.817868775090457 22.85627720257066 30.595062605969854 25.73079141636903 52 21.179911272163753 23.52889771830223 28.80887394879652 26.482317060737497 53 20.664193739200368 22.56696864355169 32.30696159192035 24.461876025327594 54 20.64178073608654 23.60588566149578 30.34397139351691 25.408362208900765 55 21.910600389454412 23.263488065670877 24.495691514791105 30.330220030083606 56 21.90657246742341 25.765028676730005 23.52658173961471 28.801817116231877 57 26.43822596445398 27.319296076876427 23.126778467136262 23.11569949153333 58 24.91288639803116 32.129829155282366 21.929591798053906 21.02769264863257 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 309.5 9 1189.0 10 1759.0 11 1919.0 12 2079.0 13 1643.5 14 1208.0 15 1077.5 16 912.0 17 877.0 18 852.5 19 828.0 20 908.5 21 989.0 22 1172.5 23 1622.5 24 1889.0 25 2181.0 26 2473.0 27 2794.5 28 3570.5 29 4025.0 30 3928.5 31 3832.0 32 4809.5 33 5787.0 34 5672.0 35 5758.0 36 5959.0 37 6183.5 38 6408.0 39 7012.0 40 7616.0 41 8448.0 42 9271.0 43 9262.0 44 9992.0 45 10722.0 46 11730.0 47 13179.5 48 13621.0 49 14821.5 50 16022.0 51 16670.5 52 17319.0 53 19788.5 54 22608.5 55 22959.0 56 26384.5 57 29810.0 58 32008.5 59 30926.0 60 27645.0 61 24044.0 62 20443.0 63 17013.0 64 13583.0 65 10955.0 66 7047.5 67 5768.0 68 4879.5 69 3991.0 70 3641.0 71 3291.0 72 2754.0 73 1734.0 74 1251.0 75 989.0 76 727.0 77 564.0 78 336.0 79 271.0 80 208.5 81 146.0 82 115.0 83 84.0 84 61.5 85 27.5 86 16.0 87 10.0 88 4.0 89 3.5 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 5.818537277913706E-4 18 2.909268638956853E-4 19 0.0017455611833741115 20 2.909268638956853E-4 21 0.004363902958435279 22 0.0 23 0.0026183417750611673 24 0.0 25 0.0 26 0.026183417750611674 27 0.006400391005705076 28 0.02531063715892462 29 0.016000977514262688 30 0.057603519051345684 31 0.05643981159576294 32 0.02531063715892462 33 0.019783026744906597 34 0.028801759525672842 35 0.03694771171475203 36 0.06429483692094644 37 0.06865873987938172 38 0.09367845017441065 39 0.07418635029339975 40 0.07796839952404365 41 0.08349600993806168 42 0.10793386650529922 43 0.11055220828036041 44 0.0866962054409142 45 0.08436879052974872 46 0.0910601083993495 47 0.13353543052811953 48 0.13266264993643248 49 0.15506401845640025 50 0.16233719005379238 51 0.13702655289486776 52 0.12568040520293602 53 0.15884606768704415 54 0.1716468496984543 55 0.19928490176854438 56 0.22343183147188628 57 0.21441309869112005 58 0.2286685150220086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 343729.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.19167014877944 #Duplication Level Percentage of deduplicated Percentage of total 1 62.35187405384133 20.695628367533402 2 14.230600822128403 9.44674817014071 3 7.49664171821062 7.46478177403278 4 4.502857209987006 5.97829404963768 5 2.918403705456164 4.843334657623832 6 1.97703605695023 3.9372677204721334 7 1.342088068002965 3.1182301124636957 8 0.8914874443356893 2.3671965755334856 9 0.6860302369046408 2.0493440401885072 >10 3.1497422944227504 18.88662431744827 >50 0.27423567940745486 6.32568742904901 >100 0.16224876209315658 9.475799574464013 >500 0.01058144100607543 2.2454390392663672 >1k 0.006172507253544 3.16562417214612 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 2779 0.8084857547661093 No Hit TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 1788 0.5201772326454852 No Hit TCACTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1440 0.4189346840097868 No Hit TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 1360 0.39566053489813197 No Hit TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1236 0.359585603775067 No Hit TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 1137 0.3307838442493941 No Hit TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1076 0.31303730555175735 No Hit TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 933 0.27143476401467437 No Hit TCACTTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 911 0.26503437300896926 No Hit TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 735 0.21383124496332867 No Hit TCACTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 731 0.21266753750774592 No Hit TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA 612 0.17804724070415937 No Hit TCACTTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT 600 0.17455611833741116 No Hit TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 565 0.16437367810106215 No Hit TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 532 0.15477309159250457 No Hit TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 532 0.15477309159250457 No Hit TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 513 0.14924548117848654 No Hit TCACTTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 507 0.14749991999511242 No Hit TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 501 0.14575435881173832 No Hit TCACTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 475 0.1381902603504505 No Hit TCACTTGGGGAACTTTGTCTGCTGGTGCCCTGTGAGGGTTTTGCATAAATTCTCCCTA 474 0.1378993334865548 No Hit TCACTTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 439 0.12771689325020583 No Hit TCACTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 431 0.12538947833904035 No Hit TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA 415 0.12073464851670937 No Hit TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAA 413 0.12015279478891801 No Hit TCACTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 399 0.11607981869437842 No Hit TCACTTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG 385 0.11200684259983884 No Hit TCACTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 383 0.11142498887204745 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.009018732780766243 0.0 0.0 0.0 0.0 11 0.02152858792828071 0.0 0.0 0.0 0.0 12 0.021819514792176396 0.0 0.0 0.0 0.0 13 0.02211044165607208 0.0 0.0 0.0 0.0 14 0.029674540117359895 0.0 0.0 0.0 0.0 15 0.030547320709046952 0.0 0.0 0.0 0.0 16 0.031711028164629694 0.0 0.0 0.0 0.0 17 0.031711028164629694 0.0 0.0 0.0 0.0 18 0.03200195502852538 0.0 0.0 0.0 0.0 19 0.03287473562021243 0.0 0.0 0.0 0.0 20 0.0453845907677269 0.0 0.0 0.0 0.0 21 0.045675517631622586 0.0 0.0 0.0 0.0 22 0.046257371359413955 0.0 0.0 0.0 0.0 23 0.04916663999837081 0.0 0.0 0.0 0.0 24 0.057312592187449996 0.0 0.0 0.0 0.0 25 0.05818537277913705 0.0 0.0 0.0 0.0 26 0.05934908023471979 0.0 0.0 0.0 0.0 27 0.06022186082640685 0.0 0.0 0.0 0.0 28 0.0928056695827236 0.0 0.0 0.0 0.0 29 0.09426030390220203 0.0 0.0 0.0 0.0 30 0.1047336710024467 0.0 0.0 0.0 0.0 31 0.10589737845802943 0.0 0.0 0.0 0.0 32 0.10647923218582081 0.0 0.0 0.0 0.0 33 0.10735201277750786 0.0 0.0 0.0 0.0 34 0.10909757396088197 0.0 0.0 0.0 0.0 35 0.13964489466992894 0.0 0.0 0.0 0.0 36 0.1419723095810944 0.0 0.0 0.0 0.0 37 0.1419723095810944 0.0 0.0 0.0 0.0 38 0.1437178707644685 0.0 0.0 0.0 0.0 39 0.14488157822005127 0.0 0.0 0.0 0.0 40 0.146045285675634 0.0 0.0 0.0 0.0 41 0.1480817737229038 0.0 0.0 0.0 0.0 42 0.14866362745069517 0.0 0.0 0.0 0.0 43 0.15070011549796497 0.0 0.0 0.0 0.0 44 0.15244567668133907 0.0 0.0 0.0 0.0 45 0.15331845727302612 0.0 0.0 2.9092686389568524E-4 0.0 46 0.1541912378647132 0.0 0.0 2.9092686389568524E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAATA 30 7.9021993E-7 52.072586 52 GTGAATC 15 0.0071761548 52.072586 52 ACGAACA 15 0.0071761548 52.072586 52 TAGACGT 15 0.0071844314 52.05741 49 AACGTAT 15 0.0071885725 52.049828 45 CCTAATA 15 0.0071885725 52.049828 43 CTACGAT 15 0.0071885725 52.049828 43 CTACGTA 15 0.0072051543 52.01951 39 TACGTAC 15 0.0072051543 52.01951 40 TGCGATC 45 9.276846E-11 51.9968 32 CGATATA 15 0.007217609 51.996796 32 ACGATAT 15 0.0072259214 51.981667 31 TACGATA 15 0.0072259214 51.981667 30 TCGTAAC 15 0.0072259214 51.981667 28 CGTAACC 15 0.0072259214 51.981667 29 TTACGAT 15 0.0072259214 51.981667 29 TCGGTTA 15 0.0072259214 51.981667 30 AAGCGAT 15 0.0072300797 51.974106 10 CACTAAT 15 0.0072300797 51.974106 12 TCATCGG 15 0.0072300797 51.974106 27 >>END_MODULE