FastQCFastQC Report
Tue 31 May 2016
SRR212935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366102
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA29050.7934947091247794No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA19400.5299069658182692No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA15070.4116339162309957No Hit
TCACTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC14330.39142097011215454No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG13150.35918951549021855No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA11900.3250460254246084No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT10710.2925414228821476No Hit
TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10440.2851664290279758No Hit
TCACTTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG10020.2736942163659308No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA7880.21524056137360625No Hit
TCACTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT7540.2059535320757603No Hit
TCACTTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT6460.17645355665907317No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA6170.1685322669638516No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA5860.1600646814275803No Hit
TCACTTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5680.15514801885813245No Hit
TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5630.15378227925550803No Hit
TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5620.15350913133498315No Hit
TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5540.1513239479707841No Hit
TCACTTGGGGAACTTTGTCTGCTGGTGCCCTGTGAGGGTTTTGCATAAATTCTCCCTA5320.14531469371923672No Hit
TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5270.14394895411661232No Hit
TCACTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5110.13957858738821421No Hit
TCACTTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4750.1297452622493185No Hit
TCACTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA4550.12428230383882087No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAA4380.11963878918989791No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA4180.11417583077940029No Hit
TCACTTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG4130.11281009117677587No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3940.10762028068680313No Hit
TCACTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3920.10707398484575337No Hit
TCACTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3820.10434250564050455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAT251.6501617E-552.01490438
ATAGGTC203.4396863E-452.014938
CGCTAAG203.4420087E-452.0077932
GATAGGT203.4420087E-452.0077937
CATAGCG150.00721584852.00068329
TGTACCG150.00721584852.00068328
CGTTCGG505.456968E-1251.97226719
CGTTAGA150.00723537151.96516812
GTACGTA150.00723537151.96516813
GATCGTA203.458302E-451.9580739
TCACTTG379550.050.1168481
CACTTGG380800.049.952342
ACTTGGG381800.049.8215073
TTGGGAT38300.049.719675
CTTGGGA139350.049.4599084
CTTGGGG191450.049.447694
TTGGGAG43400.048.7855155
TTGGGAC23150.048.7036745
TTGGGGC47650.048.6868365
TTGGGGG78600.048.652855
Produced by FastQC (version 0.11.5)