FastQCFastQC Report
Tue 31 May 2016
SRR212934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378739
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA27980.7387673305363324No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA18410.4860867246309464No Hit
TCACTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC14600.38548974359651367No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA14360.3791529258935573No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG11970.3160487829349499No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA11590.3060154882386023No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9320.24607975413147312No Hit
TCACTTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG9210.24317537935095146No Hit
TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9080.23974293642851674No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA7210.1903685651596482No Hit
TCACTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6990.18455981559860485No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA6600.17426248683130072No Hit
TCACTTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT6140.16211691956730098No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA5750.15181959079999682No Hit
TCACTTGGGGAACTTTGTCTGCTGGTGCCCTGTGAGGGTTTTGCATAAATTCTCCCTA5480.14469067088417087No Hit
TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5300.1399380576069536No Hit
TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5120.1351854443297363No Hit
TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5100.13465737618782328No Hit
TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4980.13148896733634508No Hit
TCACTTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4940.13043283105251902No Hit
TCACTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4660.12303987706573656No Hit
TCACTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA4480.11828726378851928No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAA4380.11564692307895412No Hit
TCACTTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4280.11300658236938896No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA4060.10719783280834559No Hit
TCACTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4050.10693379873738908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT150.007122095752.173269
TACGGCA150.00718211752.06266452
CCGTCCG150.00718211752.06266425
TCAATCG150.00718211752.06266425
GTCCGAC150.00718211752.06266429
GCCGAAT307.929211E-752.0488751
TCGCATT150.00719718152.03508839
AGTAGGC150.00720095152.028243
GTTATCG150.00720472252.0213141
TCGATCA150.007231161551.9731546
TACCCGT203.4602673E-451.95253835
CCCGTTG203.4602673E-451.95253837
TTGTCGA150.00724630251.94566734
TAGTTAT150.00724630251.94566733
ATATCGC150.00724630251.94566733
CTACCGT150.00724630251.94566734
AGTGCGA505.456968E-1251.93880530
CTAGTCG150.007250090651.93880530
CGTCGAC353.918467E-851.9250816
CGGTAGT251.6685419E-551.9182212
Produced by FastQC (version 0.11.5)