##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212934_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	378739
Sequences flagged as poor quality	0
Sequence length	58
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.02030157971585	39.0	38.0	39.0	35.0	40.0
2	37.332297439661616	39.0	36.0	39.0	33.0	40.0
3	37.238169293365615	39.0	36.0	39.0	33.0	40.0
4	37.3189901224854	39.0	36.0	39.0	33.0	40.0
5	37.23389458175683	39.0	36.0	39.0	33.0	40.0
6	37.654706803365904	39.0	38.0	39.0	35.0	40.0
7	36.47675840090405	38.0	35.0	39.0	31.0	40.0
8	37.704236954736636	39.0	38.0	39.0	35.0	40.0
9	37.621908491071686	39.0	38.0	39.0	34.0	40.0
10	36.08762234678763	38.0	35.0	39.0	30.0	40.0
11	36.71338309495457	38.0	36.0	39.0	32.0	40.0
12	36.535297394775824	38.0	35.0	39.0	31.0	40.0
13	36.431109550376384	38.0	35.0	39.0	31.0	40.0
14	36.415763890172386	38.0	35.0	39.0	31.0	40.0
15	36.42790153641426	38.0	35.0	39.0	31.0	40.0
16	36.02743049963167	38.0	35.0	39.0	30.0	40.0
17	35.23007400875009	38.0	34.0	39.0	28.0	40.0
18	35.62500561072401	38.0	35.0	39.0	29.0	40.0
19	35.535888831094766	38.0	34.0	39.0	29.0	40.0
20	35.452261319800705	38.0	34.0	39.0	28.0	40.0
21	35.321379630827565	38.0	34.0	39.0	28.0	40.0
22	35.244712057643916	38.0	33.0	39.0	28.0	40.0
23	35.13588513461777	38.0	33.0	39.0	28.0	40.0
24	35.126704669970614	38.0	33.0	39.0	28.0	40.0
25	35.03932259418755	38.0	33.0	39.0	28.0	40.0
26	34.59772561051278	38.0	33.0	39.0	26.0	40.0
27	34.41189843137359	38.0	33.0	39.0	26.0	40.0
28	34.191136904306134	38.0	33.0	39.0	25.0	40.0
29	33.7793625689459	37.0	33.0	39.0	23.0	40.0
30	33.81846337451385	37.0	33.0	39.0	25.0	40.0
31	33.28691790388632	36.0	32.0	39.0	22.0	40.0
32	33.21644721034802	36.0	31.0	38.0	22.0	40.0
33	33.00070233062874	36.0	31.0	38.0	22.0	40.0
34	32.58310604400392	36.0	30.0	38.0	21.0	40.0
35	32.214815479789515	36.0	30.0	38.0	18.0	40.0
36	33.596965192388424	38.0	33.0	39.0	23.0	40.0
37	33.51904345736774	38.0	33.0	39.0	23.0	40.0
38	33.27655720694198	37.0	32.0	39.0	23.0	40.0
39	33.02939227277888	37.0	32.0	39.0	21.0	40.0
40	33.15726133300241	37.0	32.0	39.0	22.0	40.0
41	33.322462170518484	38.0	33.0	39.0	22.0	40.0
42	33.24445594459509	38.0	33.0	39.0	22.0	40.0
43	33.168332281597614	38.0	33.0	39.0	21.0	40.0
44	32.722423621544124	37.0	32.0	39.0	18.0	40.0
45	32.76124719133757	37.0	32.0	39.0	19.0	40.0
46	32.27231417942171	37.0	31.0	39.0	14.0	40.0
47	32.33765469096132	36.0	31.0	39.0	17.0	40.0
48	32.15775243637439	36.0	31.0	39.0	16.0	40.0
49	32.094402741729795	36.0	31.0	39.0	15.0	40.0
50	31.657038224212453	36.0	30.0	39.0	9.0	40.0
51	31.615666725634277	36.0	31.0	39.0	2.0	40.0
52	31.5580914561215	36.0	30.0	39.0	2.0	40.0
53	31.40926073100473	36.0	30.0	39.0	2.0	40.0
54	31.260995038799805	36.0	30.0	39.0	2.0	40.0
55	31.120251677276435	36.0	30.0	39.0	2.0	40.0
56	30.53890145984438	36.0	30.0	39.0	2.0	40.0
57	30.400845965163345	36.0	30.0	39.0	2.0	40.0
58	30.1361412476666	35.0	29.0	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2	1	0.9723963011835437
2	2	1.8118475812837005
2	3	0.27783841069001625
2	4	1.869007054463033
2	5	1.632973373041061
2	6	1.8470745131661772
2	7	-0.8510380442950876
2	8	1.6143204402635334
2	9	1.7095209781439422
2	10	1.4567591581140888
2	11	1.1011988083981663
2	12	-2.524598146831501
2	13	1.491203709557638
2	14	-1.5195860096522296
2	15	1.414470387416472
2	16	0.15787745226632666
2	17	0.6614844933778627
2	18	-3.2738763005502207
2	19	-0.4207801709801018
2	20	-0.7636224885454794
2	21	2.460762694419813
2	22	2.171561779819868
2	23	1.5229476185683382
2	24	1.320421218804249
2	25	2.212265914003794
2	26	4.198110726150947
2	27	2.1992019400746443
2	28	2.18441984115449
2	29	4.844883043305298
2	30	6.047497384552781
2	31	5.897414048688411
2	32	6.093225311368183
2	33	3.449014739593757
2	34	8.079207623066747
2	35	7.791618555948332
2	36	5.303308004321433
2	37	5.877153381628972
2	38	6.867432622221202
2	39	6.18361605803215
2	40	6.457686288819268
2	41	-6.79183934553593
2	42	-5.638847399486032
2	43	-4.775406133284431
2	44	-4.439945028057547
2	45	-4.968638358784624
2	46	-4.495263842441592
2	47	-4.529039369980325
2	48	-5.313698346184847
2	49	-4.5128005422589546
2	50	-7.780527669205167
2	51	-5.256732834288194
2	52	-5.677617972218762
2	53	-1.6577115635775073
2	54	-3.025958400565532
2	55	-3.9719239511160858
2	56	-4.931581785898764
2	57	-3.512499715311435
2	58	-4.607139484759546
3	1	-1.360937032149792
3	2	-4.771485752049635
3	3	-2.4721615893099838
3	4	-3.4643262788703026
3	5	-3.367026626958939
3	6	-1.2362588201671585
3	7	-0.5177047109617519
3	8	-0.7190128930698023
3	9	-0.6238123551893935
3	10	-0.45990750855257545
3	11	-8.648801191601834
3	12	-11.0245981468315
3	13	-7.508796290442362
3	14	-6.51958600965223
3	15	-6.585529612583528
3	16	-0.8421225477336733
3	17	-4.0051821732888016
3	18	-0.7738763005502207
3	19	-0.8374468376467661
3	20	-1.5969558218788151
3	21	-5.289237305580187
3	22	-2.9951048868467964
3	23	-0.9770523814316618
3	24	-2.5962454478624153
3	25	-0.6210674193295418
3	26	-8.718555940515717
3	27	-6.96746472659202
3	28	-1.6489134921788455
3	29	-6.571783623361366
3	30	-12.869169282113884
3	31	-8.102585951311589
3	32	-15.406774688631817
3	33	-6.800985260406243
3	34	-10.670792376933253
3	35	-9.458381444051668
3	36	0.5533080043214333
3	37	-0.20617995170436387
3	38	-0.8825673777787983
3	39	1.1836160580321504
3	40	-0.37564704451406783
3	41	0.6248273211307378
3	42	-3.722180732819364
3	43	3.224593866715569
3	44	-6.439945028057547
3	45	-3.301971692117956
3	46	3.4214028242250762
3	47	-3.112372703313657
3	48	-1.3970316795181787
3	49	0.7371994577410454
3	50	-0.530527669205167
3	51	2.0766004990451385
3	52	-6.677617972218762
3	53	1.5922884364224927
3	54	-3.109291733898864
3	55	-0.47192395111608576
3	56	4.318418214101236
3	57	3.987500284688565
3	58	1.9761938485737858
4	1	-2.860937032149792
4	2	-7.521485752049635
4	3	-8.222161589309984
4	4	-7.964326278870303
4	5	-13.367026626958939
4	6	-7.9862588201671585
4	7	-9.517704710961752
4	8	-3.2190128930698023
4	9	-3.1238123551893935
4	10	-5.2099075085525755
4	11	-5.898801191601834
4	12	-10.0245981468315
4	13	-6.008796290442362
4	14	-9.01958600965223
4	15	-8.585529612583528
4	16	-1.8421225477336733
4	17	-6.0051821732888016
4	18	-2.2738763005502207
4	19	-6.587446837646766
4	20	-3.596955821878815
4	21	-17.539237305580187
4	22	-19.495104886846796
4	23	-19.477052381431662
4	24	-20.346245447862415
4	25	-21.621067419329542
4	26	-13.968555940515717
4	27	-15.46746472659202
4	28	-15.148913492178846
4	29	-14.821783623361366
4	30	-14.119169282113884
4	31	-13.602585951311589
4	32	-15.906774688631817
4	33	-21.050985260406243
4	34	-14.420792376933253
4	35	-11.708381444051668
4	36	-17.196691995678567
4	37	-15.956179951704364
4	38	-14.132567377778798
4	39	-16.31638394196785
4	40	-25.375647044514068
4	41	-15.625172678869262
4	42	-14.472180732819364
4	43	-20.77540613328443
4	44	-15.439945028057547
4	45	-21.801971692117956
4	46	-21.828597175774924
4	47	-17.862372703313657
4	48	-17.14703167951818
4	49	-19.512800542258955
4	50	-22.280527669205167
4	51	-20.42339950095486
4	52	-15.677617972218762
4	53	-18.657711563577507
4	54	-14.359291733898864
4	55	-16.471923951116086
4	56	-8.431581785898764
4	57	-8.012499715311435
4	58	-6.773806151426214
5	1	-2.360937032149792
5	2	-3.021485752049635
5	3	-8.972161589309984
5	4	-6.714326278870303
5	5	-3.367026626958939
5	6	-2.2362588201671585
5	7	-3.017704710961752
5	8	-2.7190128930698023
5	9	-6.1238123551893935
5	10	-1.2099075085525755
5	11	-2.6488011916018337
5	12	-3.774598146831501
5	13	-0.758796290442362
5	14	-0.26958600965222956
5	15	-6.085529612583528
5	16	-4.842122547733673
5	17	-6.0051821732888016
5	18	-5.023876300550221
5	19	-0.5874468376467661
5	20	-1.3469558218788151
5	21	-5.789237305580187
5	22	-0.49510488684679643
5	23	-1.2270523814316618
5	24	-1.0962454478624153
5	25	-0.3710674193295418
5	26	-2.968555940515717
5	27	3.28253527340798
5	28	-1.3989134921788455
5	29	-3.071783623361366
5	30	0.8808307178861163
5	31	-8.852585951311589
5	32	-11.656774688631817
5	33	-2.550985260406243
5	34	-7.670792376933253
5	35	-0.20838144405166759
5	36	-7.196691995678567
5	37	-5.456179951704364
5	38	-2.6325673777787983
5	39	-1.3163839419678496
5	40	-12.375647044514068
5	41	-6.625172678869262
5	42	-4.472180732819364
5	43	-5.275406133284431
5	44	-3.689945028057547
5	45	-8.301971692117956
5	46	-10.328597175774924
5	47	-6.862372703313657
5	48	-7.647031679518179
5	49	-7.2628005422589546
5	50	-8.030527669205167
5	51	-21.92339950095486
5	52	-20.427617972218762
5	53	-18.657711563577507
5	54	-18.859291733898864
5	55	-22.971923951116086
5	56	-17.931581785898764
5	57	-24.512499715311435
5	58	-24.273806151426214
6	1	-1.0752227464355073
6	2	-2.3786286091924893
6	3	-0.8650187321671297
6	4	-1.6071834217274485
6	5	-0.36702662695893906
6	6	-0.7719731058814432
6	7	-4.803418996676037
6	8	-1.147584321641233
6	9	-1.1952409266179629
6	10	-5.6384789371240025
6	11	-1.1845154773161184
6	12	-3.3817410039743585
6	13	-4.223082004728077
6	14	-1.3767288667950837
6	15	-1.2998153268692434
6	16	-4.413551119162246
6	17	-3.5766107447173745
6	18	-4.131019157693078
6	19	-2.8017325519324814
6	20	-1.8112415361645304
6	21	0.603619837276959
6	22	-3.2093906011325117
6	23	-1.3341952385745195
6	24	-0.48910259071955764
6	25	0.4503611520990276
6	26	-2.325698797658575
6	27	-5.46746472659202
6	28	-3.1489134921788455
6	29	-1.1074979090756507
6	30	-2.333454996399599
6	31	-6.388300237025874
6	32	-1.6210604029175322
6	33	-2.97955668897767
6	34	-3.7779352340761108
6	35	-2.6369528726230946
6	36	-2.5538348528214243
6	37	-0.7418942374186486
6	38	-1.8468530920645136
6	39	-2.530669656253565
6	40	-1.5899327587997831
6	41	-2.125172678869262
6	42	0.027819267180635876
6	43	-0.9182632761415732
6	44	-0.1542307423432625
6	45	-0.4448288349750982
6	46	0.3142599670822186
6	47	-3.862372703313657
6	48	-2.075603108089606
6	49	-2.7985148279732392
6	50	-0.9233848120623094
6	51	-0.9233995009548615
6	52	0.036667742066953224
6	53	-2.3719972778632226
6	54	-0.35929173389886415
6	55	0.1709331917410566
6	56	2.4969896426726628
6	57	1.7732145704028497
6	58	3.7261938485737858
7	1	-0.735937032149792
7	2	-1.3964857520496352
7	3	-1.8471615893099838
7	4	-1.3393262788703026
7	5	-0.7420266269589391
7	6	-0.2362588201671585
7	7	-5.017704710961752
7	8	-0.4690128930698023
7	9	-0.3738123551893935
7	10	-0.5849075085525755
7	11	0.1011988083981663
7	12	-1.6495981468315009
7	13	-2.258796290442362
7	14	-3.6445860096522296
7	15	-0.3355296125835281
7	16	-1.5921225477336733
7	17	-0.5051821732888016
7	18	-2.2738763005502207
7	19	-0.5874468376467661
7	20	1.2780441781211849
7	21	-4.414237305580187
7	22	-2.8701048868467964
7	23	-7.227052381431662
7	24	-2.7212454478624153
7	25	-1.7460674193295418
7	26	-4.718555940515717
7	27	-1.59246472659202
7	28	-4.3989134921788455
7	29	-4.946783623361366
7	30	-0.7441692821138837
7	31	-1.602585951311589
7	32	-2.156774688631817
7	33	-1.050985260406243
7	34	-2.295792376933253
7	35	0.5416185559483324
7	36	1.1783080043214333
7	37	-7.706179951704364
7	38	-4.382567377778798
7	39	-2.6913839419678496
7	40	-5.250647044514068
7	41	-2.500172678869262
7	42	-2.472180732819364
7	43	-3.650406133284431
7	44	-3.189945028057547
7	45	-2.676971692117956
7	46	-6.703597175774924
7	47	-5.362372703313657
7	48	-7.397031679518179
7	49	-4.8878005422589546
7	50	-5.155527669205167
7	51	-4.2983995009548615
7	52	-7.177617972218762
7	53	-4.657711563577507
7	54	-3.484291733898864
7	55	-6.221923951116086
7	56	-7.806581785898764
7	57	-7.012499715311435
7	58	-5.898806151426214
8	1	-0.47204814326090627
8	2	-4.632596863160746
8	3	-0.4999393670877623
8	4	0.09122927668525449
8	5	-1.7003599602922748
8	6	-0.4862588201671585
8	7	-1.073260266517309
8	8	-0.8301240041809166
8	9	-0.7349234663005078
8	10	-2.098796397441461
8	11	-0.45435674715739083
8	12	-0.3023759246092794
8	13	0.15787037622430233
8	14	0.258191768125549
8	15	-3.1410851681390852
8	16	-3.8421225477336733
8	17	-4.227404395511023
8	18	-5.496098522772442
8	19	-3.9763357265356554
8	20	-3.541400266323258
8	21	-5.98368175002463
8	22	-4.828438220180129
8	23	-3.5881634925427726
8	24	-7.012912114529083
8	25	-5.343289641551763
8	26	-4.801889273849049
8	27	-2.023020282147577
8	28	-5.482246825512178
8	29	-2.155116956694698
8	30	-6.396947059891662
8	31	-3.547030395756032
8	32	-5.128996910854038
8	33	-5.7732074826284645
8	34	-4.1430145991554745
8	35	-3.986159221829446
8	36	-2.918914217900788
8	37	-3.456179951704364
8	38	-2.6881229333343555
8	39	-2.4830506086345174
8	40	-4.764535933402957
8	41	-3.4585060122025943
8	42	-2.083291843930475
8	43	-2.775406133284431
8	44	-1.6621672502797686
8	45	-3.9686383587846237
8	46	-3.8285971757749238
8	47	-4.751261592202546
8	48	-2.3136983461848466
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120	28	5.3510865078211545
120	29	5.678216376638634
120	30	7.380830717886116
120	31	7.897414048688411
120	32	8.093225311368183
120	33	8.449014739593757
120	34	-1.920792376933253
120	35	7.291618555948332
120	36	-1.1966919956785667
120	37	2.043820048295636
120	38	1.3674326222212017
120	39	-0.8163839419678496
120	40	1.6243529554859322
120	41	8.374827321130738
120	42	7.027819267180636
120	43	7.224593866715569
120	44	7.560054971942453
120	45	6.698028307882044
120	46	4.671402824225076
120	47	7.637627296686343
120	48	8.352968320481821
120	49	9.487199457741045
120	50	7.219472330794833
120	51	9.076600499045139
120	52	8.322382027781238
120	53	8.842288436422493
120	54	7.640708266101136
120	55	10.028076048883914
120	56	4.568418214101236
120	57	5.987500284688565
120	58	7.226193848573786
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	273.0
3	27.0
4	141.0
5	148.0
6	316.0
7	479.0
8	390.0
9	734.0
10	881.0
11	878.0
12	1154.0
13	1286.0
14	1378.0
15	1683.0
16	1817.0
17	1957.0
18	2066.0
19	2120.0
20	2130.0
21	2154.0
22	2782.0
23	2916.0
24	3479.0
25	4306.0
26	5397.0
27	6719.0
28	8215.0
29	9732.0
30	11460.0
31	13484.0
32	15664.0
33	18637.0
34	22898.0
35	29125.0
36	37533.0
37	51661.0
38	58349.0
39	54215.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	49.25217760781814	36.5222009772679	3.77868068833652	10.446940726577438
11	33.1539448736975	22.143736103555906	22.83321098301003	21.869108039736567
12	34.81368435782953	14.057966039937794	26.2505313685678	24.877818233664872
13	21.761952162307026	14.813367516944387	31.732142715696032	31.692537605052557
14	20.554788389893833	20.137614557782534	36.925692891410705	22.381904160912924
15	29.38593596117643	19.346304447125856	30.52365877292806	20.744100818769653
16	32.4561241382588	20.933677281716434	25.939235198910065	20.6709633811147
17	31.5081055652753	21.484901501657056	24.238732286419545	22.7682606466481
18	30.87490560757041	21.956075175979173	24.40288537194578	22.766133844504644
19	26.65397542898936	26.551002141316317	24.399652531162623	22.395369898531705
20	28.18623956402579	26.522028420702437	24.119312031008246	21.172419984263527
21	26.688968862297585	24.548433345566977	27.30390746085014	21.4586903312853
22	25.118425847200292	21.600240812425074	29.786543021456595	23.494790318918035
23	30.05085309633573	22.662632215410124	26.152115710596775	21.13439897765738
24	31.516426879724555	22.99076672853865	22.464546825122316	23.028259566614476
25	26.68761635660245	21.8152290162008	21.434437593250326	30.062717033946416
26	25.05170787888073	22.61331487786903	25.65978725146539	26.675189991784855
27	22.415796813590838	21.538900393018636	23.98998436377467	32.05531842961585
28	24.87597149061399	27.587163379492896	21.493255702141372	26.04360942775174
29	30.883333686193975	23.10593229310016	21.758357504287257	24.252376516418607
30	26.925941774037625	22.535222428247675	25.470028579428096	25.068807218286608
31	33.195655216671376	20.939045742761998	23.568574529724476	22.296724510842154
32	35.1803875274985	20.148313645330735	24.380375907483554	20.29092291968721
33	33.30040752293142	20.174365936048215	26.44514815294127	20.080078388079095
34	26.112437537635884	23.19796739807938	30.812829479066522	19.87676558521821
35	23.95599461158509	32.10517974589926	25.836657069653185	18.10216857286246
36	26.473627050209146	33.117269188794964	22.663745881084566	17.74535787991132
37	30.52965345946017	29.00688324899922	21.828486874265103	18.6349764172755
38	28.18421094356974	30.13947686924902	21.51426744253648	20.162044744644756
39	29.51171487849035	26.59125447649756	21.47053770829754	22.42649293671455
40	25.56169828173972	25.49431034391632	22.597950349624476	26.34604102471948
41	22.305149070112243	28.94505244741384	23.75766096955698	24.992137512916944
42	21.807440692526267	25.88303707130113	23.659551972510407	28.649970263662194
43	23.69314952616344	22.556999167316512	24.96464399476599	28.785207311754053
44	24.42848175194082	22.683268573184264	23.93953600478881	28.948713670086107
45	27.146498027728132	24.490276118061356	24.39615697803534	23.96706887617517
46	23.318817242683124	27.81536801859329	25.282323420614965	23.583491318108624
47	23.684962079944153	23.924011804402323	26.970838049100394	25.420188066553134
48	25.595453740113232	22.408153227363236	28.22399162252715	23.772401409996377
49	27.966422203238768	22.648879814444058	25.349702597412904	24.034995384904274
50	21.499111355605866	23.092588282588917	28.3855764551552	27.02272390665002
51	20.696413975892142	22.898762336298542	30.847689861949846	25.557133825859474
52	20.86426677814172	23.577237923389124	29.140760182240545	26.417735116228613
53	20.319038071911848	22.823045223950867	32.489935832667676	24.36798087146961
54	20.296046279715064	23.59429597750556	30.491814553139935	25.617843189639437
55	21.563185424874128	23.418413108300108	24.684688021716642	30.333713445109122
56	21.55196392950146	26.2843624746015	23.624904962607587	28.538768633289447
57	26.17104897510884	27.548068652489775	23.434212441609656	22.846669930791727
58	24.925872585564214	32.33702558454761	21.96262919349373	20.774472636394442
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	318.5
9	1167.0
10	1697.0
11	1957.0
12	2217.0
13	1845.5
14	1474.0
15	1281.5
16	1079.0
17	1069.0
18	990.0
19	911.0
20	1050.0
21	1189.0
22	1300.0
23	1772.0
24	2133.0
25	2409.5
26	2686.0
27	3097.0
28	3950.0
29	4392.0
30	4353.5
31	4315.0
32	5336.5
33	6358.0
34	6357.0
35	6583.0
36	6810.0
37	7170.5
38	7531.0
39	8120.5
40	8710.0
41	9451.0
42	10352.5
43	10513.0
44	11254.0
45	11995.0
46	13080.5
47	14768.0
48	15370.0
49	16538.0
50	17706.0
51	18521.0
52	19336.0
53	22292.0
54	25562.0
55	25876.0
56	29574.0
57	33272.0
58	35184.0
59	33632.0
60	30168.0
61	25912.5
62	21657.0
63	17965.0
64	14273.0
65	11448.0
66	7357.0
67	6091.0
68	5178.0
69	4265.0
70	3972.5
71	3680.0
72	2899.5
73	1617.5
74	1116.0
75	883.0
76	650.0
77	515.0
78	304.5
79	229.0
80	178.0
81	127.0
82	90.0
83	53.0
84	39.5
85	19.0
86	12.0
87	8.5
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5753302406142489
11	0.01188153319304323
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0942601633314763
18	2.6403407095651623E-4
19	0.0
20	2.6403407095651623E-4
21	5.280681419130325E-4
22	0.005544715490086841
23	2.6403407095651623E-4
24	0.0
25	0.014257839831651877
26	0.04488579206260776
27	0.03458846329530363
28	0.05201471197843369
29	0.23102981208695172
30	0.03828494028869485
31	0.020594657534608266
32	0.021650793818434332
33	0.029043747805216784
34	0.029307781876173306
35	0.039341076572520915
36	0.0797382894288679
37	0.07498567615165061
38	0.08581107306086777
39	0.09874874253773706
40	0.0879233456285199
41	0.09505226554434584
42	0.10931010537599771
43	0.11723112750469322
44	0.3147286125801673
45	0.13069686512347553
46	0.14205033017460572
47	0.15181959079999682
48	0.15366782929669245
49	0.16686953284451825
50	0.1676616350573878
51	0.18165544081808319
52	0.20515447313321308
53	0.1755826571860833
54	0.1821835089599962
55	0.20594657534608266
56	0.3316267931213844
57	0.23604645943512548
58	0.2669384457370379
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	378739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.18604016691104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.64571811283292	25.15476390863347
2	12.627581274485552	9.39139488967907
3	6.44254498849047	7.187182101574141
4	3.807468404548859	5.663386921031942
5	2.4062796731703506	4.474000628966711
6	1.6464626876511852	3.6735256581790368
7	1.1345736234287034	2.9533210233196296
8	0.7250096567731974	2.1568190574533204
9	0.5403418301746464	1.8083855700662965
>10	2.651278687141306	17.754753490936178
>50	0.2310256478965748	6.1275556045414765
>100	0.12842959339937385	8.708676899283157
>500	0.008857213337887852	2.2314457811835253
>1k	0.004428606668943926	2.71478846515206
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	2798	0.7387673305363324	No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	1841	0.4860867246309464	No Hit
TCACTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	1460	0.38548974359651367	No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA	1436	0.3791529258935573	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	1197	0.3160487829349499	No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	1159	0.3060154882386023	No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	932	0.24607975413147312	No Hit
TCACTTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	921	0.24317537935095146	No Hit
TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	908	0.23974293642851674	No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA	721	0.1903685651596482	No Hit
TCACTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	699	0.18455981559860485	No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA	660	0.17426248683130072	No Hit
TCACTTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	614	0.16211691956730098	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	575	0.15181959079999682	No Hit
TCACTTGGGGAACTTTGTCTGCTGGTGCCCTGTGAGGGTTTTGCATAAATTCTCCCTA	548	0.14469067088417087	No Hit
TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	530	0.1399380576069536	No Hit
TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	512	0.1351854443297363	No Hit
TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	510	0.13465737618782328	No Hit
TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	498	0.13148896733634508	No Hit
TCACTTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	494	0.13043283105251902	No Hit
TCACTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	466	0.12303987706573656	No Hit
TCACTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	448	0.11828726378851928	No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAA	438	0.11564692307895412	No Hit
TCACTTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	428	0.11300658236938896	No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA	406	0.10719783280834559	No Hit
TCACTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	405	0.10693379873738908	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.007392953986782454	0.0	0.0	0.0	0.0
11	0.0195385212507822	0.0	0.0	0.0	0.0
12	0.020066589392695233	0.0	0.0	0.0	0.0
13	0.020066589392695233	0.0	0.0	0.0	0.0
14	0.03406039515339059	0.0	0.0	0.0	0.0
15	0.03406039515339059	0.0	0.0	0.0	0.0
16	0.03406039515339059	0.0	0.0	0.0	0.0
17	0.03485249736626014	0.0	0.0	0.0	0.0
18	0.03485249736626014	0.0	0.0	0.0	0.0
19	0.03564459957912969	0.0	0.0	0.0	0.0
20	0.045941928346433826	0.0	0.0	0.0	0.0
21	0.04673403055930337	0.0	0.0	0.0	0.0
22	0.04752613277217292	0.0	0.0	0.0	0.0
23	0.0504305075526946	0.0	0.0	0.0	0.0
24	0.05597522304278144	0.0	0.0	0.0	0.0
25	0.05597522304278144	0.0	0.0	0.0	0.0
26	0.05676732525565099	0.0	0.0	0.0	0.0
27	0.05729539339756402	0.0	0.0	0.0	0.0
28	0.08475493677704171	0.0	0.0	0.0	0.0
29	0.08581107306086777	0.0	0.0	0.0	0.0
30	0.09399612926051978	0.0	0.0	0.0	0.0
31	0.09505226554434584	0.0	0.0	0.0	0.0
32	0.09584436775721539	0.0	0.0	0.0	0.0
33	0.09690050404104146	0.0	0.0	0.0	0.0
34	0.097692606253911	0.0	0.0	0.0	0.0
35	0.1370336828264319	0.0	0.0	0.0	0.0
36	0.1415222620326927	0.0	0.0	0.0	0.0
37	0.14205033017460572	0.0	0.0	0.0	0.0
38	0.14389856867130135	0.0	0.0	0.0	0.0
39	0.1465389093808665	0.0	0.0	0.0	0.0
40	0.14891521601947516	0.0	0.0	0.0	0.0
41	0.15049942044521425	0.0	0.0	0.0	0.0
42	0.15102748858712728	0.0	0.0	0.0	0.0
43	0.15208362487095334	0.0	0.0	0.0	0.0
44	0.15393186336764897	0.0	0.0	0.0	0.0
45	0.15551606779338806	0.0	0.0	0.0	0.0
46	0.15657220407721412	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	15	0.0071220957	52.17326	9
TACGGCA	15	0.007182117	52.062664	52
CCGTCCG	15	0.007182117	52.062664	25
TCAATCG	15	0.007182117	52.062664	25
GTCCGAC	15	0.007182117	52.062664	29
GCCGAAT	30	7.929211E-7	52.04887	51
TCGCATT	15	0.007197181	52.035088	39
AGTAGGC	15	0.007200951	52.0282	43
GTTATCG	15	0.007204722	52.02131	41
TCGATCA	15	0.0072311615	51.97315	46
TACCCGT	20	3.4602673E-4	51.952538	35
CCCGTTG	20	3.4602673E-4	51.952538	37
TTGTCGA	15	0.007246302	51.945667	34
TAGTTAT	15	0.007246302	51.945667	33
ATATCGC	15	0.007246302	51.945667	33
CTACCGT	15	0.007246302	51.945667	34
AGTGCGA	50	5.456968E-12	51.938805	30
CTAGTCG	15	0.0072500906	51.938805	30
CGTCGAC	35	3.918467E-8	51.92508	16
CGGTAGT	25	1.6685419E-5	51.91822	12
>>END_MODULE