FastQCFastQC Report
Tue 31 May 2016
SRR212933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836125
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC119651.431006129466288No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC54200.6482284347436089No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT51180.6121094333981163No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT33710.4031693825683959No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCAGT33660.40257138585737784No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC30810.3684855733293467No Hit
TCACTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC29560.35353565555389443No Hit
TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24080.28799521602631184No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG19460.2327403199282404No Hit
TCACTTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG15150.1811930034384811No Hit
TCACTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA14420.17246225145761698No Hit
TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13370.1599043205262371No Hit
TCACTTGGGGAACTTTGTCTGCTGGTGCCCTGTGAGGGTTTTGCATAAATTCTCCC12880.15404395275825983No Hit
TCACTTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC11890.14220361788010166No Hit
TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11350.1357452534011063No Hit
TCACTTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT11170.1335924652414412No Hit
TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC10580.12653610405142773No Hit
TCACTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT10360.12390491852294812No Hit
TCACTTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG10180.12175213036328301No Hit
TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9980.11936014351921063No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA9740.11648975930632381No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCA9690.11589176259530572No Hit
TCACTTGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9630.11517416654208401No Hit
TCACTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC9460.11314097772462252No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC9350.11182538496038272No Hit
TCACTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA8910.10656301390342351No Hit
TCACTTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC8790.10512782179698012No Hit
TCACTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8670.10369262969053672No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG8590.10273583495290776Illumina Single End Adapter 2 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGA150.00825263550.26999748
ATTGCGA402.582965E-950.2124543
CGCATTC204.118588E-450.1580540
ATAGTGC451.3460522E-1050.0766732
TATGCGA150.008395215550.05261230
GTGCGAT2400.049.0186831
TGCGATC2150.047.74752832
TTGGGAT93650.047.7350545
TCACTTG880850.047.3052181
CTTGGGG422600.047.1718864
CACTTGG883500.047.1633262
ACTTGGG886450.047.006373
GTGGTAT55450.046.82620215
TTGGGGC98850.046.8120845
TTGGGAC47250.046.725585
TTGGGGG175100.046.692045
CGCGATC700.046.3827125
CTTGGGA349250.046.1555064
TGGTATC56150.046.06492616
AGTGCGA2500.046.048430
Produced by FastQC (version 0.11.5)