FastQCFastQC Report
Tue 31 May 2016
SRR212931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313476
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22860.7292424300424913No Hit
ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16880.5384782248082788No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT15800.5040258265385548No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15380.4906276716558844No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA11480.3662162334596588No Hit
ACCTGAGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACT9720.310071584427516No Hit
ACCTGAGGGATTTGGGCGTATGGAATCATGGGGTTGTTCTACTTTTAGTCTCGGAGTT6880.21947453712564918No Hit
ACCTGAGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5700.18183210197909888No Hit
ACCTGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5300.1690719544717937No Hit
ACCTGAGGGGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCCAACGATGTTGAA5270.1681149434087458No Hit
ACCTGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC5010.15982084752899745No Hit
ACCTGAGGGTGCAGTCTCAGTCTTTATTTATTTTCCCAGCCACCATCCCCATAGAACA4990.15918284015363218No Hit
ACCTGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4970.15854483277826692No Hit
ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4610.14706070002169225No Hit
ACCTGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4370.13940461151730915No Hit
ACCTGAGGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA4350.1387666041419439No Hit
ACCTGAGGGATCTTTCTTAGAACAAATGCAGTTCAATTGCATCCCATTTCCAGTCATT4230.1349385598897523No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3920.1250494455715908No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3830.12217841238244713No Hit
ACCTGAGGGATCCAGAAGCTGGGCATGGTAGTATGTCTGTAATCCTAGCAATGGGGAC3780.12058339394403399No Hit
ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3770.12026439025635137No Hit
ACCTGAGGGGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGTCACCCAGACA3760.11994538656866874No Hit
ACCTGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3730.11898837550562084No Hit
ACCTGAGGGGTGTAGTATGTATGCAAAGTATAGTTATCAGCCTTGAGGGTTATAAAAC3690.11771236075489033No Hit
ACCTGAGGGAGGTTTCCACTGCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCCA3630.11579833862879454No Hit
ACCTGAGGGAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCA3630.11579833862879454No Hit
ACCTGAGGGATCTGATCTCTCTCTTTCTTCTGAAACCCTCCCCTCTTGCTGCTGCTCC3570.11388431650269878No Hit
ACCTGAGGGAGCTGAGACACTTTTCCGTTATTGCCTTTGTCCTAGATAATGGTGACCT3550.11324630912733351No Hit
ACCTGAGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3500.11165129068892035No Hit
ACCTGAGGGGTTATAGATCCTTCTAGATCTGATGTTCTTATATGAAATGGCTTGCAAG3420.10909926118745933No Hit
ACCTGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3380.1078232464367288No Hit
ACCTGAGGGACTCTTCTAGTTCCTGGGCCTATCTGATCTCTCTCTTTCTTCTGAAACC3380.1078232464367288No Hit
ACCTGAGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTG3380.1078232464367288No Hit
ACCTGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG3340.10654723168599829No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.10240018374612411No Hit
ACCTGAGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA3140.10016715793234571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACC203.420714E-452.0708550
AACTCGC251.6391456E-552.07084751
CTAGCGA150.00717651152.07084349
TAGGTTA203.423416E-452.06252348
ACGGGTC203.423416E-452.06252348
AACGGGT150.00719013852.0458747
GCGACAT203.4315325E-452.0375637
CACGGAT203.4315325E-452.0375633
ACGGATC203.4315325E-452.0375634
CCAACGA401.875378E-952.0375645
GAAATCG150.007194684452.03755640
ATGATCG150.007194684452.03755640
GCGAACC150.007194684452.03755642
CGCGACG150.007194684452.03755644
CCGTAAG150.007194684452.03755644
CTACGTA150.007194684452.03755639
ACCCGTT150.007194684452.03755636
CTCTACG150.007194684452.03755637
CTCGCTA203.4342412E-452.02924332
TATGCGT307.945473E-752.0292432
Produced by FastQC (version 0.11.5)