##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212931_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 313476 Sequences flagged as poor quality 0 Sequence length 58 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.091129783460296 39.0 38.0 40.0 35.0 40.0 2 37.88393688831043 39.0 38.0 40.0 34.0 40.0 3 37.98176893924894 39.0 38.0 40.0 35.0 40.0 4 37.715429570365835 39.0 38.0 40.0 33.0 40.0 5 37.62180198803098 39.0 38.0 40.0 33.0 40.0 6 37.364672255611275 39.0 37.0 40.0 33.0 40.0 7 38.022882134517474 39.0 38.0 40.0 35.0 40.0 8 37.837100766884866 39.0 38.0 40.0 35.0 40.0 9 37.840561956896224 39.0 38.0 40.0 35.0 40.0 10 36.81038420804145 39.0 35.0 40.0 31.0 40.0 11 37.12936556546594 39.0 36.0 40.0 32.0 40.0 12 37.323520779900214 39.0 37.0 40.0 32.0 40.0 13 37.32404394594801 39.0 37.0 40.0 33.0 40.0 14 37.31383901797905 39.0 37.0 40.0 33.0 40.0 15 37.297218925850785 39.0 37.0 40.0 33.0 40.0 16 37.29928925978384 39.0 37.0 40.0 33.0 40.0 17 37.11256045119882 39.0 36.0 40.0 33.0 40.0 18 37.03975104952213 39.0 36.0 40.0 32.0 40.0 19 37.08623945692812 39.0 36.0 40.0 33.0 40.0 20 36.960545623907414 39.0 36.0 40.0 32.0 40.0 21 37.03832191300131 39.0 36.0 40.0 33.0 40.0 22 36.95075859076931 39.0 36.0 40.0 32.0 40.0 23 36.76587362349909 38.0 36.0 40.0 31.0 40.0 24 36.5989485638454 38.0 36.0 40.0 31.0 40.0 25 36.661907131646444 38.0 36.0 40.0 31.0 40.0 26 36.47217649836032 38.0 36.0 40.0 31.0 40.0 27 36.41280034197195 38.0 36.0 40.0 31.0 40.0 28 36.16757901721344 38.0 35.0 40.0 30.0 40.0 29 36.18517526062601 38.0 35.0 40.0 30.0 40.0 30 35.961891819469436 38.0 35.0 40.0 30.0 40.0 31 35.774598374357204 38.0 35.0 40.0 29.0 40.0 32 35.58828746060304 38.0 35.0 40.0 29.0 40.0 33 35.49243961260192 38.0 35.0 39.0 29.0 40.0 34 35.266718983271446 38.0 35.0 39.0 28.0 40.0 35 35.12559494187753 38.0 35.0 39.0 28.0 40.0 36 35.77626995368067 38.0 35.0 40.0 30.0 40.0 37 35.686068470951525 38.0 35.0 40.0 29.0 40.0 38 35.52736094629254 38.0 35.0 40.0 29.0 40.0 39 35.50964348147865 38.0 35.0 40.0 29.0 40.0 40 35.31620921537853 38.0 35.0 40.0 28.0 40.0 41 35.335001722619914 38.0 35.0 40.0 28.0 40.0 42 35.26506973420613 38.0 35.0 40.0 28.0 40.0 43 35.11016154346744 38.0 35.0 40.0 28.0 40.0 44 35.068761244879994 38.0 35.0 40.0 28.0 40.0 45 34.83204455843509 38.0 35.0 40.0 27.0 40.0 46 34.59676657862165 38.0 35.0 40.0 26.0 40.0 47 34.44380750041471 38.0 34.0 39.0 26.0 40.0 48 34.364149089563476 38.0 34.0 39.0 26.0 40.0 49 34.23648700378976 38.0 34.0 39.0 25.0 40.0 50 33.999961719557476 38.0 33.0 39.0 24.0 40.0 51 33.742193979762405 38.0 33.0 39.0 23.0 40.0 52 33.64467136240095 38.0 33.0 39.0 23.0 40.0 53 33.47921052967372 38.0 33.0 39.0 22.0 40.0 54 33.24348275466065 38.0 33.0 39.0 19.0 40.0 55 33.048211027319475 38.0 33.0 39.0 18.0 40.0 56 32.73373400196506 38.0 33.0 39.0 2.0 40.0 57 32.50094106087867 38.0 33.0 39.0 2.0 40.0 58 32.24705559596269 37.0 32.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -2.040269002549941 1 2 -2.6987450508402944 1 3 -3.1099934691121476 1 4 -3.2547291950790225 1 5 -3.0009514958708863 1 6 -2.8399637441415777 1 7 -3.6233378338259 1 8 -3.60288205334534 1 9 -3.601453975723622 1 10 -3.422443836115626 1 11 -2.4454639045654716 1 12 -2.3767062664202285 1 13 -2.5840307798704742 1 14 -2.3186803005940604 1 15 -2.2992789190707015 1 16 -2.187815781133139 1 17 -2.410311972915366 1 18 -2.838358220266173 1 19 -2.3269003812612112 1 20 -2.86742985889461 1 21 -2.290045192996679 1 22 -2.601406613695417 1 23 -2.2172197065707664 1 24 -2.3613578260910586 1 25 -2.0041404211627665 1 26 -2.0349394017834115 1 27 -2.2216196519508387 1 28 -1.7944688148128591 1 29 -2.518288891903012 1 30 -2.6311550333300744 1 31 -2.2425865241366836 1 32 -2.4757847752332225 1 33 -2.67384849193769 1 34 -3.053132544597723 1 35 -2.7245952028561007 1 36 -2.1610418604231185 1 37 -2.5785752351498274 1 38 -2.2100484006674037 1 39 -2.1365381977309 1 40 -2.308198782450013 1 41 -2.6146832641574775 1 42 -2.7968341411353563 1 43 -2.501460325302631 1 44 -2.4099921501830366 1 45 -2.246166921679176 1 46 -2.2609970270284876 1 47 -2.2038037830379267 1 48 -2.2411090221910044 1 49 -2.0286195129011233 1 50 -2.1030641023347947 1 51 -1.8642334100360536 1 52 -1.7430354891459352 1 53 -1.8562504350425364 1 54 -1.2004701489464935 1 55 -1.4490075662340907 1 56 -1.638688763782401 1 57 -1.8436776568440472 1 58 -2.0114573582651047 2 1 -1.0320253030916717 2 2 -1.6305343261798555 2 3 -1.8824359594944937 2 4 -1.7682567133329243 2 5 -1.8802637358017975 2 6 -1.0352530587368491 2 7 -1.6830222179037833 2 8 -1.767677531087351 2 9 -1.7583040974163282 2 10 -1.6507393531133516 2 11 -1.2991814204640377 2 12 -1.2072943169815815 2 13 -1.0341053657227164 2 14 -1.2150923285441309 2 15 -1.0940657605561412 2 16 -1.248222470306409 2 17 -1.1442210566870585 2 18 -1.3068986929049444 2 19 -1.4510897508144822 2 20 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117 57 0.5619399767086861 117 58 0.6222025223410483 118 1 -0.5559693901161822 118 2 -0.8965462056295053 118 3 -1.041765991456522 118 4 -1.0849277239092032 118 5 -1.53667877578863 118 6 -0.9995461217418296 118 7 -1.3907822694819956 118 8 -1.5368376018702108 118 9 -1.5608939820512333 118 10 -1.3635337673028403 118 11 -0.8060745191348033 118 12 -0.7828440501740843 118 13 -0.7125920390652851 118 14 -0.7647294977782764 118 15 -0.9588375681255172 118 16 -0.7052246239710698 118 17 -0.6759293105725703 118 18 -0.8333119496025674 118 19 -0.5729021213545451 118 20 -0.40284075820648724 118 21 -0.45533824053286764 118 22 -0.5636782264455604 118 23 -0.48358053865175776 118 24 -0.6835440160776116 118 25 -0.6244362370292507 118 26 -0.5385856882660462 118 27 -0.9261201660693175 118 28 -0.8333361722438539 118 29 -1.0021139334279638 118 30 -0.7872730432960608 118 31 -1.0660303270315694 118 32 -0.9507353407635577 118 33 -0.7537345949195782 118 34 -0.35878784792762985 118 35 -0.668912216223184 118 36 5.914408530571791E-5 118 37 -0.12083577457876515 118 38 -0.14512710034311738 118 39 -0.20020979466200828 118 40 -0.016084569051294295 118 41 -0.141994819959109 118 42 -0.3190203311219122 118 43 -0.17943232561901112 118 44 -0.17781624256617334 118 45 -0.3330133979901788 118 46 0.07579012791891415 118 47 0.37960205418425375 118 48 0.17627609210148876 118 49 0.001958672656101612 118 50 0.077272843010455 118 51 0.15894146934384423 118 52 0.0432494115896489 118 53 0.12430797672682559 118 54 -0.01562477996760947 118 55 -0.06319723640731212 118 56 0.5301635655698078 118 57 0.06571093763511016 118 58 0.3787348429529587 119 1 -0.976674749586131 119 2 -0.9773977948145429 119 3 -1.3326204613395163 119 4 -1.3143821745875357 119 5 -1.695761890783352 119 6 -0.828610992316186 119 7 -1.2328377160587607 119 8 -1.2879727340393785 119 9 -1.9095956103314577 119 10 -1.6507393531133516 119 11 -0.6829452581024142 119 12 -1.0375526195646074 119 13 -0.7056920815898735 119 14 -1.052730704927896 119 15 -0.8689735096336335 119 16 -0.39951398322153864 119 17 -1.0150697651741396 119 18 -0.8825444493625056 119 19 -1.0488757286742612 119 20 -0.9270043269797199 119 21 -0.7105680790766016 119 22 -0.5995223395335287 119 23 -0.9651509305638584 119 24 -0.7824616967042317 119 25 -0.6801708835950464 119 26 -0.8112917610516845 119 27 -0.7525924084869118 119 28 -0.6883684772137428 119 29 -0.5792137564708071 119 30 -0.7220869639259746 119 31 -1.4770373367299214 119 32 -1.342880637681212 119 33 -1.1281155878001385 119 34 -1.3505024119134177 119 35 -1.2441052915740087 119 36 -0.9941108719642955 119 37 -0.9509706186781344 119 38 -0.7341749610819477 119 39 -0.9952961470125246 119 40 -0.5863804578837915 119 41 -0.4823287065693478 119 42 -0.5692295246636618 119 43 -0.3481589199481476 119 44 -0.4053913807710856 119 45 -0.173779389293216 119 46 -0.4564119599012244 119 47 -0.26417906764183385 119 48 -0.531758311662621 119 49 -0.43472770164258634 119 50 -0.4650802756880168 119 51 -0.12010213893610455 119 52 -0.34121402412277035 119 53 0.15370481344611875 119 54 0.31527139144763083 119 55 0.27708177976575143 119 56 1.1634781515037744 119 57 1.09560160828903 119 58 1.1759337071349094 120 1 -2.316689631216434 120 2 -3.185269873535333 120 3 -3.2488429568978034 120 4 -3.5607794533751402 120 5 -3.913428511894004 120 6 -2.6999760022625807 120 7 -4.355550424667328 120 8 -4.416471056059635 120 9 -4.427248564638781 120 10 -4.080302167260008 120 11 -2.175557591633158 120 12 -2.2053202231665665 120 13 -2.396989364933077 120 14 -1.9843663175803172 120 15 -2.2287597000881263 120 16 -2.3925439135208464 120 17 -2.2776090572337253 120 18 -2.3911469427948475 120 19 -2.149630439925076 120 20 -2.165687454551737 120 21 -2.7221089352259042 120 22 -2.566646084845054 120 23 -2.7322534164035304 120 24 -2.2700173130011336 120 25 -2.392530229410731 120 26 -2.461451966357444 120 27 -2.4830678613895856 120 28 -2.2437114380507595 120 29 -2.3267119721936993 120 30 -2.205588095233587 120 31 -1.9591262316929416 120 32 -2.858028011377172 120 33 -2.88976653764297 120 34 -2.775205918207739 120 35 -2.5767097287294902 120 36 -2.4466035450391743 120 37 -2.159662705599004 120 38 -2.2141986502077273 120 39 -2.520830291242852 120 40 -2.5339758597637356 120 41 -2.5123804885686525 120 42 -2.6009668790742424 120 43 -2.605671865447974 120 44 -2.923712489908098 120 45 -2.88849246234987 120 46 -2.3935598498986437 120 47 -2.525579459423529 120 48 -2.920821831927455 120 49 -2.708139695021778 120 50 -2.401286674789045 120 51 -2.149409839420521 120 52 -2.0906407776495683 120 53 -1.9274000716768036 120 54 -1.9610865573170386 120 55 -2.115884372117989 120 56 -2.1536524272094226 120 57 -2.0055023657136744 120 58 -1.569954862861902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 337.0 3 22.0 4 64.0 5 62.0 6 96.0 7 153.0 8 157.0 9 227.0 10 270.0 11 367.0 12 461.0 13 590.0 14 577.0 15 680.0 16 787.0 17 893.0 18 937.0 19 1000.0 20 1108.0 21 1167.0 22 1394.0 23 1579.0 24 1853.0 25 2091.0 26 2663.0 27 3215.0 28 4000.0 29 5012.0 30 6041.0 31 7246.0 32 8931.0 33 11412.0 34 14701.0 35 19807.0 36 28399.0 37 44181.0 38 60390.0 39 80140.0 40 466.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 43.18384820528525 45.035345608595236 3.7342571680128627 8.04654901810665 11 30.13723538644107 24.194196684913678 28.137720272046344 17.53084765659891 12 30.900292207377916 16.593933825875027 27.161249984049817 25.344523982697243 13 23.309599458969746 18.63013436435325 31.726192754788247 26.33407342188876 14 22.56408784085544 25.169709961847158 30.311411399915784 21.95479079738162 15 26.09035460449923 27.01163725452666 27.998315660529038 18.899692480445076 16 29.67528303873061 26.287112446686002 23.816405240642734 20.221199273940655 17 27.31437175413748 26.5261136418737 23.504829715831516 22.654684888157306 18 25.07783689979456 25.19969630848933 27.11084740139596 22.611619390320154 19 24.830527669049644 27.954879686607775 25.54829283542761 21.666299808914964 20 26.04236450059017 28.33604491657894 24.833636392637253 20.787954190193638 21 25.474376642931883 24.74829645509532 28.962815506724855 20.81451139524794 22 23.92471488954462 23.864742004944574 28.210862110216127 23.999680995294682 23 23.37382682651388 27.10533199772862 28.078643297837726 21.442197877919774 24 23.706834675811468 28.772948400988913 26.350107664088046 21.170109259111573 25 23.32083898237499 30.659223223542547 23.482574367971928 22.537363426110534 26 24.604109033939892 29.610697910384896 25.20428707718717 20.580905978488037 27 22.445535489263417 24.89336791498783 26.28397153074864 26.37712506500011 28 21.84348147935432 27.456629877443707 27.387390836835166 23.3124978063668 29 24.881552121852156 25.256756713641686 27.163089567334232 22.698601597171926 30 22.48953173671041 24.83659296328448 29.91657304805188 22.757302251953224 31 26.08298813452222 24.736234067133456 28.2007748622928 20.98000293605152 32 25.16327044988307 25.128175678046944 30.22329846189186 19.48525541017812 33 25.028951339418164 24.244154700644746 29.91925527265303 20.80763868728406 34 21.575703045733867 27.31429191313947 31.83567203323315 19.27433300789351 35 20.942906187981393 28.94637763469405 31.460623967021256 18.650092210303303 36 20.95539897113826 28.87131569205632 30.566512209755036 19.606773127050385 37 23.89873078383752 26.801619950022822 30.475549158589786 18.824100107549874 38 21.47152898814426 29.141049459564716 29.62306793587558 19.76435361641544 39 23.57255475198039 26.73224350668003 29.522982746184432 20.172218995155145 40 21.41872267083719 27.660464077112124 29.64922919791899 21.27158405413169 41 20.691559135838325 27.715027080853183 31.12654434270285 20.466869440605638 42 20.4289609286704 26.044466863757975 30.145392086124218 23.381180121447407 43 19.795610836903705 26.289643899293164 31.320516176818014 22.594229086985116 44 21.028340158505717 25.612111036989987 30.549216248910792 22.8103325555935 45 21.688342775073803 26.3097422803798 30.564429905050666 21.437485039495733 46 21.490724071449545 28.476398411664817 30.277320258934388 19.75555725795125 47 20.69541446715016 27.83573962834159 31.17357578312368 20.295270121384572 48 21.830536184126498 26.184318083122587 30.65273201011572 21.332413722635195 49 21.982331862053105 27.11701468512261 29.900417111138083 21.0002363416862 50 20.255384114188253 26.948836941906183 30.799400824664563 21.996378119241005 51 19.653354535671976 26.435867715288353 32.11515679831914 21.79562095072053 52 19.920181347977394 27.209539925289743 31.43418153954216 21.436097187190704 53 20.019992846010375 26.361493650834205 32.20381205447252 21.414701448682898 54 20.091668396761264 27.306003162080582 31.015538911158313 21.58678952999984 55 20.324995447124337 27.75560802456316 29.79210131985469 22.127295208457806 56 20.643664645096145 28.96299183916053 28.876718281686358 21.516625234056967 57 20.869756920481557 27.975231960739205 28.685811415280014 22.46919970349922 58 21.13258432710866 30.869345705995883 28.255492925342228 19.74257704155323 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 288.5 10 577.0 11 1965.0 12 3353.0 13 4107.0 14 4861.0 15 3898.5 16 2563.0 17 2190.0 18 2100.0 19 2010.0 20 1903.5 21 1797.0 22 1908.5 23 2564.0 24 3108.0 25 3775.5 26 4443.0 27 5003.5 28 5837.0 29 6110.0 30 6338.0 31 6566.0 32 6667.5 33 6769.0 34 8220.0 35 8307.5 36 6944.0 37 7424.5 38 7905.0 39 8854.0 40 9803.0 41 9881.5 42 9657.0 43 9354.0 44 10321.0 45 11288.0 46 12072.0 47 13106.0 48 13356.0 49 13461.5 50 13567.0 51 14568.5 52 15570.0 53 16061.5 54 17623.0 55 18693.0 56 18072.0 57 17451.0 58 18330.5 59 18874.5 60 18539.0 61 17550.0 62 16561.0 63 13576.5 64 10592.0 65 8995.5 66 6102.5 67 4806.0 68 4382.5 69 3959.0 70 3203.5 71 2448.0 72 2171.5 73 1486.0 74 1077.0 75 837.5 76 598.0 77 511.5 78 382.0 79 339.0 80 267.5 81 196.0 82 140.0 83 84.0 84 61.5 85 27.5 86 16.0 87 13.5 88 11.0 89 8.0 90 5.0 91 3.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 9.570110630478889E-4 17 0.0 18 0.0 19 9.570110630478889E-4 20 0.0019140221260957777 21 0.0038280442521915555 22 3.1900368768262964E-4 23 0.003190036876826296 24 3.1900368768262964E-4 25 3.1900368768262964E-4 26 0.022011254450101443 27 0.004785055315239445 28 0.022011254450101443 29 0.014993173321083592 30 0.047212545777029186 31 0.04147047939874185 32 0.012441143819622556 33 0.006061070065969963 34 0.017226199134862 35 0.019778228636323034 36 0.03828044252191556 37 0.04242749046178974 38 0.06699077441335222 39 0.04912656790312496 40 0.05295461215531652 41 0.049445571590807584 42 0.08102693667138791 43 0.07975092192065741 44 0.055825645344460176 45 0.04816955684007707 46 0.06124870803506489 47 0.10814225012441145 48 0.09570110630478888 49 0.11803136444257295 50 0.12090239763171663 51 0.09665811736783676 52 0.08485498092357947 53 0.11611734231647718 54 0.12473044188390818 55 0.15535479590144063 56 0.16524391021960214 57 0.15694981433985378 58 0.16843394709642842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 313476.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.431854432237238 #Duplication Level Percentage of deduplicated Percentage of total 1 59.587663863790674 14.558371294772169 2 10.899879876743093 5.326085569549184 3 6.067530161382984 4.44723040998354 4 4.093330547866506 4.000306243540176 5 3.0866454274821122 3.7706235884086823 6 2.394631012691283 3.5103165792596562 7 2.023815741369405 3.461190011356531 8 1.689559722149684 3.302326174890582 9 1.4192823941087376 3.1208130765991657 >10 8.117459654253931 33.58789827610407 >50 0.3747323340471092 6.303193864921078 >100 0.2311067007886353 10.774668555168498 >500 0.007834125450462213 1.2083859689418008 >1k 0.006528437875385177 2.628590386504868 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACCTGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2286 0.7292424300424913 No Hit ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1688 0.5384782248082788 No Hit ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 1580 0.5040258265385548 No Hit ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 1538 0.4906276716558844 No Hit ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 1148 0.3662162334596588 No Hit ACCTGAGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACT 972 0.310071584427516 No Hit ACCTGAGGGATTTGGGCGTATGGAATCATGGGGTTGTTCTACTTTTAGTCTCGGAGTT 688 0.21947453712564918 No Hit ACCTGAGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 570 0.18183210197909888 No Hit ACCTGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 530 0.1690719544717937 No Hit ACCTGAGGGGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCCAACGATGTTGAA 527 0.1681149434087458 No Hit ACCTGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC 501 0.15982084752899745 No Hit ACCTGAGGGTGCAGTCTCAGTCTTTATTTATTTTCCCAGCCACCATCCCCATAGAACA 499 0.15918284015363218 No Hit ACCTGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 497 0.15854483277826692 No Hit ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 461 0.14706070002169225 No Hit ACCTGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 437 0.13940461151730915 No Hit ACCTGAGGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 435 0.1387666041419439 No Hit ACCTGAGGGATCTTTCTTAGAACAAATGCAGTTCAATTGCATCCCATTTCCAGTCATT 423 0.1349385598897523 No Hit ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 392 0.1250494455715908 No Hit ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 383 0.12217841238244713 No Hit ACCTGAGGGATCCAGAAGCTGGGCATGGTAGTATGTCTGTAATCCTAGCAATGGGGAC 378 0.12058339394403399 No Hit ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 377 0.12026439025635137 No Hit ACCTGAGGGGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGTCACCCAGACA 376 0.11994538656866874 No Hit ACCTGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 373 0.11898837550562084 No Hit ACCTGAGGGGTGTAGTATGTATGCAAAGTATAGTTATCAGCCTTGAGGGTTATAAAAC 369 0.11771236075489033 No Hit ACCTGAGGGAGGTTTCCACTGCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCCA 363 0.11579833862879454 No Hit ACCTGAGGGAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCA 363 0.11579833862879454 No Hit ACCTGAGGGATCTGATCTCTCTCTTTCTTCTGAAACCCTCCCCTCTTGCTGCTGCTCC 357 0.11388431650269878 No Hit ACCTGAGGGAGCTGAGACACTTTTCCGTTATTGCCTTTGTCCTAGATAATGGTGACCT 355 0.11324630912733351 No Hit ACCTGAGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 350 0.11165129068892035 No Hit ACCTGAGGGGTTATAGATCCTTCTAGATCTGATGTTCTTATATGAAATGGCTTGCAAG 342 0.10909926118745933 No Hit ACCTGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 338 0.1078232464367288 No Hit ACCTGAGGGACTCTTCTAGTTCCTGGGCCTATCTGATCTCTCTCTTTCTTCTGAAACC 338 0.1078232464367288 No Hit ACCTGAGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTG 338 0.1078232464367288 No Hit ACCTGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG 334 0.10654723168599829 No Hit ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 321 0.10240018374612411 No Hit ACCTGAGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA 314 0.10016715793234571 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0025520295014610367 0.0 0.0 0.0 0.0 11 0.004466051627556814 0.0 0.0 0.0 0.0 12 0.004466051627556814 0.0 0.0 0.0 0.0 13 0.005104059002922073 0.0 0.0 0.0 0.0 14 0.006061070065969963 0.0 0.0 0.0 0.0 15 0.006380073753652593 0.0 0.0 0.0 0.0 16 0.010527121693526776 0.0 0.0 0.0 0.0 17 0.010846125381209406 0.0 0.0 0.0 0.0 18 0.011803136444257296 0.0 0.0 0.0 0.0 19 0.012441143819622556 0.0 0.0 0.0 0.0 20 0.014355165945718333 0.0 0.0 0.0 0.0 21 0.014993173321083592 0.0 0.0 0.0 0.0 22 0.01658819175949674 0.0 0.0 0.0 0.0 23 0.019140221260957775 0.0 0.0 0.0 0.0 24 0.020416236011688293 0.0 0.0 0.0 0.0 25 0.022011254450101443 0.0 0.0 0.0 0.0 26 0.02392527657619722 0.0 0.0 0.0 0.0 27 0.02520129132692774 0.0 0.0 0.0 0.0 28 0.03190036876826296 0.0 0.0 0.0 0.0 29 0.03317638351899348 0.0 0.0 0.0 0.0 30 0.039237453584963444 0.0 0.0 0.0 0.0 31 0.03955645727264607 0.0 0.0 0.0 0.0 32 0.04115147571105922 0.0 0.0 0.0 0.0 33 0.04178948308642448 0.0 0.0 0.0 0.0 34 0.043384501524837624 0.0 0.0 0.0 0.0 35 0.054549630593729664 0.0 0.0 0.0 0.0 36 0.05518763796909492 0.0 0.0 0.0 0.0 37 0.05742066378287333 0.0 0.0 0.0 0.0 38 0.05869667853360385 0.0 0.0 0.0 0.0 39 0.060929704347382256 0.0 0.0 0.0 0.0 40 0.06316273016116067 0.0 0.0 0.0 0.0 41 0.06539575597493907 0.0 0.0 0.0 0.0 42 0.06667177072566959 0.0 0.0 0.0 0.0 43 0.06858579285176537 0.0 0.0 0.0 0.0 44 0.07081881866554378 0.0 0.0 0.0 0.0 45 0.07241383710395692 0.0 0.0 0.0 0.0 46 0.07337084816700482 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCACC 20 3.420714E-4 52.07085 50 AACTCGC 25 1.6391456E-5 52.070847 51 CTAGCGA 15 0.007176511 52.070843 49 TAGGTTA 20 3.423416E-4 52.062523 48 ACGGGTC 20 3.423416E-4 52.062523 48 AACGGGT 15 0.007190138 52.04587 47 GCGACAT 20 3.4315325E-4 52.03756 37 CACGGAT 20 3.4315325E-4 52.03756 33 ACGGATC 20 3.4315325E-4 52.03756 34 CCAACGA 40 1.875378E-9 52.03756 45 GAAATCG 15 0.0071946844 52.037556 40 ATGATCG 15 0.0071946844 52.037556 40 GCGAACC 15 0.0071946844 52.037556 42 CGCGACG 15 0.0071946844 52.037556 44 CCGTAAG 15 0.0071946844 52.037556 44 CTACGTA 15 0.0071946844 52.037556 39 ACCCGTT 15 0.0071946844 52.037556 36 CTCTACG 15 0.0071946844 52.037556 37 CTCGCTA 20 3.4342412E-4 52.029243 32 TATGCGT 30 7.945473E-7 52.02924 32 >>END_MODULE