FastQCFastQC Report
Tue 31 May 2016
SRR212930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313043
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23020.7353622345811917No Hit
ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16560.5290008081956792No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT16440.5251674690058554No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15190.48523685244519127No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA11420.364806112898228No Hit
ACCTGAGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACT9290.2967643422788563No Hit
ACCTGAGGGATTTGGGCGTATGGAATCATGGGGTTGTTCTACTTTTAGTCTCGGAGTT7030.22456978753717538No Hit
ACCTGAGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5380.17186137367709867No Hit
ACCTGAGGGTGCAGTCTCAGTCTTTATTTATTTTCCCAGCCACCATCCCCATAGAACA5140.16419469529745115No Hit
ACCTGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5100.1629169155675099No Hit
ACCTGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5040.16100024597259802No Hit
ACCTGAGGGGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCCAACGATGTTGAA5010.16004191117514208No Hit
ACCTGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC4730.1510974530655533No Hit
ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4500.1437502196183911No Hit
ACCTGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4120.13161131218394917No Hit
ACCTGAGGGGTGTAGTATGTATGCAAAGTATAGTTATCAGCCTTGAGGGTTATAAAAC4010.12809741792661072No Hit
ACCTGAGGGATCTTTCTTAGAACAAATGCAGTTCAATTGCATCCCATTTCCAGTCATT3830.12234740914187509No Hit
ACCTGAGGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3800.12138907434441915No Hit
ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3730.11915295981702195No Hit
ACCTGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3700.11819462501956601No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3620.11563906555968351No Hit
ACCTGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3520.11244461623483035No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3510.11212517130234503No Hit
ACCTGAGGGGTTATAGATCCTTCTAGATCTGATGTTCTTATATGAAATGGCTTGCAAG3510.11212517130234503No Hit
ACCTGAGGGAGCTGAGACACTTTTCCGTTATTGCCTTTGTCCTAGATAATGGTGACCT3480.1111668365048891No Hit
ACCTGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG3420.10925016690997721No Hit
ACCTGAGGGAGGTTTCCACTGCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCCA3360.10733349731506533No Hit
ACCTGAGGGATCCAGAAGCTGGGCATGGTAGTATGTCTGTAATCCTAGCAATGGGGAC3360.10733349731506533No Hit
ACCTGAGGGATCTGATCTCTCTCTTTCTTCTGAAACCCTCCCCTCTTGCTGCTGCTCC3320.10605571758512408No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.10605571758512408No Hit
ACCTGAGGGAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCA3320.10605571758512408No Hit
ACCTGAGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTG3300.10541682772015346No Hit
ACCTGAGGGGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGTCACCCAGACA3270.10445849292269752No Hit
ACCTGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3180.1015834885303297No Hit
ACCTGAGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3160.10094459866535907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCC150.00716292452.09578724
TAGATCT353.815512E-852.09578724
CCGTTAT600.052.09578725
GCAACGT307.884355E-752.08744421
AGCGATA150.007167462752.08744421
ACCGTCC150.007194740752.03743751
ACGATGT353.858986E-852.02079848
AGAATCG203.43699E-452.02079849
AGTTTCG203.4397055E-452.01247845
GTTTCGA203.4397055E-452.01247846
CCAACGA353.8638973E-852.01247445
TATGCAC150.007212968552.00415841
TGCGAAA150.007212968552.00415844
ACGCTAA150.007212968552.00415838
TACACCG150.007212968552.00415839
CTACGTA251.6516255E-552.00415439
GAACGAT150.00721753151.99584243
GTAGCGA203.4478615E-451.98753729
ACCTAGC203.4478615E-451.98753731
CGCTAGG150.007222095551.98753432
Produced by FastQC (version 0.11.5)