FastQCFastQC Report
Tue 31 May 2016
SRR212929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312068
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19850.6360793160465027No Hit
ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16180.5184767422484843No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT14810.47457605393696245No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA13260.4249073919786713No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA10450.3348629144929951No Hit
ACCTGAGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACT8460.2710947613981568No Hit
ACCTGAGGGATTTGGGCGTATGGAATCATGGGGTTGTTCTACTTTTAGTCTCGGAGTT5940.19034313034338668No Hit
ACCTGAGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5090.16310547701142059No Hit
ACCTGAGGGTGCAGTCTCAGTCTTTATTTATTTTCCCAGCCACCATCCCCATAGAACA4660.1493264288552495No Hit
ACCTGAGGGGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCCAACGATGTTGAA4610.14772421395336913No Hit
ACCTGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4570.14644244203186485No Hit
ACCTGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.14291756924772805No Hit
ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4370.1400335824243434No Hit
ACCTGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC4320.13843136752246307No Hit
ACCTGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4150.13298383685606982No Hit
ACCTGAGGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3950.1265749772485484No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3660.11728213081764231No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3620.11600035889613802No Hit
ACCTGAGGGGTGTAGTATGTATGCAAAGTATAGTTATCAGCCTTGAGGGTTATAAAAC3420.10959149928861658No Hit
ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3310.1060666265044798No Hit
ACCTGAGGGATCTTTCTTAGAACAAATGCAGTTCAATTGCATCCCATTTCCAGTCATT3300.10574618352410371No Hit
ACCTGAGGGAGCTGAGACACTTTTCCGTTATTGCCTTTGTCCTAGATAATGGTGACCT3260.10446441160259944No Hit
ACCTGAGGGGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGTCACCCAGACA3210.10286219670071907No Hit
ACCTGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3190.10222131073996693No Hit
ACCTGAGGGATCCAGAAGCTGGGCATGGTAGTATGTCTGTAATCCTAGCAATGGGGAC3160.10125998179883872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTC150.00704843852.3083849
ATACCAT150.00704843852.30838410
AACACGG150.00704843852.30838410
CTACGTA203.4255104E-452.05597339
CGGTATT150.007184581452.05597338
AGCTATT150.007184581452.05597339
TCCATCG150.00719372752.03923425
AAGCGTG150.00719372752.03923425
CGTTAGG150.00719372752.03923424
TCGGTAG251.6450262E-552.03923426
TACGGTA203.4445967E-451.9974334
CGAAGGA251.6544443E-551.9890850
GACACGT150.007221214551.9890751
TCTTCGT150.007225803651.98072441
CGTACTG203.4500656E-451.98072442
CCGACTA150.007225803651.98072443
ACGTACT203.4500656E-451.98072441
GTTGCGA150.007239583451.9556949
GCGTTAG150.00724418151.9473523
ATCGCCC150.00724418151.9473523
Produced by FastQC (version 0.11.5)