FastQCFastQC Report
Tue 31 May 2016
SRR212928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296450
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21840.7367178276269186No Hit
ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17600.5936920222634509No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC17000.5734525215044696No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT16700.5633327711249789No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC12650.42671614100185534No Hit
ACCTGAGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGA7490.2526564344746163No Hit
ACCTGAGGGATTTGGGCGTATGGAATCATGGGGTTGTTCTACTTTTAGTCTCGGAG5710.19261258222297184No Hit
ACCTGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.16596390622364648No Hit
ACCTGAGGGTGCAGTCTCAGTCTTTATTTATTTTCCCAGCCACCATCCCCATAGAA4860.16393995614774837No Hit
ACCTGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4760.16056670602125148No Hit
ACCTGAGGGGTGTAGTATGTATGCAAAGTATAGTTATCAGCCTTGAGGGTTATAAA4690.15820543093270367No Hit
ACCTGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG4510.15213358070500926No Hit
ACCTGAGGGGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCCAACGATGTTG4330.1460617304773149No Hit
ACCTGAGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4280.14437510541406645No Hit
ACCTGAGGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3810.1285208298195311No Hit
ACCTGAGGGATCTTTCTTAGAACAAATGCAGTTCAATTGCATCCCATTTCCAGTCA3710.12514757969303425No Hit
ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3400.1146905043008939No Hit
ACCTGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA3370.11367852926294485No Hit
ACCTGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.11266655422499579No Hit
ACCTGAGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3240.1092933040984989No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.1069320290099511No Hit
ACCTGAGGGATCCAGAAGCTGGGCATGGTAGTATGTCTGTAATCCTAGCAATGGGG3150.10625737898465172No Hit
ACCTGAGGGAGGTTTCCACTGCTGTGGTTGAGCCCTACAATTCCATCCTCACCACC3130.10558272895935233No Hit
ACCTGAGGGGTTATAGATCCTTCTAGATCTGATGTTCTTATATGAAATGGCTTGCA3110.10490807893405296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACA204.055851E-450.30349350
CCTTCGT204.0592597E-450.2949348
AGTTTCG150.00827561150.22654745
CCAACGA550.050.22654745
CGTGGTA252.036853E-550.19242541
AGTACGT150.00833135350.1413338
AGCGATC252.0512238E-550.13282837
TCGTAGG150.00834253450.1243236
CAAGCCG252.0532836E-550.1243236
ACCCGTT150.00834253450.1243236
TTCGTAG150.00837614650.07336835
ATACTCA150.00837614650.07336835
CCGAAGG150.00838175850.06488434
TACATCC204.1695373E-450.0225130
CACTAGC150.00840985950.0225130
GTTTGCG252.0864743E-549.9886727
CTAATAG204.190477E-449.9717626
CGGAAAG150.00845496549.9548725
AAGGCGC204.1974752E-449.9548725
CCGTTAT301.051747E-649.9548725
Produced by FastQC (version 0.11.5)