FastQCFastQC Report
Tue 31 May 2016
SRR212927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263459
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18640.707510466524203No Hit
ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14470.5492315692384773No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT14250.5408811238181273No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC14170.5378445982107273No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC12340.468384074941452No Hit
ACCTGAGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGA6870.26076163653547607No Hit
ACCTGAGGGATTTGGGCGTATGGAATCATGGGGTTGTTCTACTTTTAGTCTCGGAG4900.1859871934532508No Hit
ACCTGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4370.1658702113042257No Hit
ACCTGAGGGTGCAGTCTCAGTCTTTATTTATTTTCCCAGCCACCATCCCCATAGAA4260.16169498859405068No Hit
ACCTGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4180.15865846298665068No Hit
ACCTGAGGGGTGTAGTATGTATGCAAAGTATAGTTATCAGCCTTGAGGGTTATAAA4000.15182628037000065No Hit
ACCTGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG3780.1434758349496506No Hit
ACCTGAGGGGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCCAACGATGTTG3760.14271670354780058No Hit
ACCTGAGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3750.1423371378468756No Hit
ACCTGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA3600.13664365233300058No Hit
ACCTGAGGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3600.13664365233300058No Hit
ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3590.1362640866320756No Hit
ACCTGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3340.12677494410895052No Hit
ACCTGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3200.1214610242960005No Hit
ACCTGAGGGATCTTTCTTAGAACAAATGCAGTTCAATTGCATCCCATTTCCAGTCA3110.11804493298767552No Hit
ACCTGAGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3080.1169062358849005No Hit
ACCTGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA2910.11045361896917547No Hit
ACCTGAGGGGTTATAGATCCTTCTAGATCTGATGTTCTTATATGAAATGGCTTGCA2800.10627839625900046No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.10513969915622544No Hit
ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2750.10438056775437544No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA2740.10400100205345045No Hit
ACCTGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT2680.10172360784790042No Hit
ACCTGAGGGACTCTTCTAGTTCCTGGGCCTATCTGATCTCTCTCTTTCTTCTGAAA2680.10172360784790042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACC252.0038953E-550.3283550
AGTCTCG507.2759576E-1250.2897947
CAACGAT950.050.26091446
TCTCGAC150.00825224150.26091446
CATTTCG150.00825224150.26091446
CGATACA150.00825224150.26091446
ACGATAC150.00825848850.25129345
TGCGAAA150.00825848850.25129344
TCCAACG950.050.25129344
CCAACGA950.050.25129345
GTCGATG252.0221487E-550.2512944
CTCGAAT204.075845E-450.2512945
TCGCGAC150.00827099250.23206743
GTCGCGA204.0835512E-450.23206342
CGTCGCG150.00830231550.1840641
TCGAATG150.00832743850.1457240
TACGTAC204.1183722E-450.1457240
CTACGTA204.1222558E-450.13614739
CGGTATT451.3278623E-1050.12657538
GCGGTAT451.3278623E-1050.1170137
Produced by FastQC (version 0.11.5)