##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212927_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 263459 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.32072922162462 39.0 38.0 40.0 35.0 40.0 2 38.16014636053428 39.0 38.0 40.0 35.0 40.0 3 38.24585988711716 39.0 38.0 40.0 35.0 40.0 4 38.10903404324772 39.0 38.0 40.0 35.0 40.0 5 38.06898606614312 39.0 38.0 40.0 35.0 40.0 6 37.45255618521288 39.0 38.0 40.0 33.0 40.0 7 38.49473352589967 39.0 38.0 40.0 36.0 40.0 8 38.446949999810215 39.0 38.0 40.0 36.0 40.0 9 38.43965474703844 39.0 38.0 40.0 36.0 40.0 10 37.03791861352241 39.0 36.0 40.0 31.0 40.0 11 37.07005644141973 39.0 36.0 40.0 32.0 40.0 12 37.35419173381816 39.0 37.0 40.0 33.0 40.0 13 37.41867994640533 39.0 37.0 40.0 33.0 40.0 14 37.43450404047689 39.0 37.0 40.0 33.0 40.0 15 37.41759818415769 39.0 37.0 40.0 33.0 40.0 16 37.39733696704231 39.0 37.0 40.0 33.0 40.0 17 37.20450620400138 39.0 37.0 40.0 33.0 40.0 18 37.12445200201929 39.0 36.0 40.0 33.0 40.0 19 37.16936980706675 39.0 36.0 40.0 33.0 40.0 20 37.03234279337582 39.0 36.0 40.0 32.0 40.0 21 36.881028926702065 39.0 36.0 40.0 32.0 40.0 22 36.82509612501376 39.0 36.0 40.0 32.0 40.0 23 36.57290887766218 38.0 36.0 40.0 31.0 40.0 24 36.448821258715775 38.0 36.0 40.0 31.0 40.0 25 36.41534356389419 38.0 36.0 40.0 31.0 40.0 26 35.9588019388216 38.0 35.0 40.0 30.0 40.0 27 35.875874424483506 38.0 35.0 40.0 30.0 40.0 28 35.59430120056631 38.0 35.0 40.0 29.0 40.0 29 35.52175860380552 38.0 35.0 40.0 29.0 40.0 30 35.25499223788142 38.0 35.0 40.0 29.0 40.0 31 34.84054824469842 38.0 34.0 39.0 27.0 40.0 32 34.607456947760376 38.0 34.0 39.0 27.0 40.0 33 34.47960024140379 38.0 33.0 39.0 26.0 40.0 34 34.71668836517257 38.0 34.0 40.0 27.0 40.0 35 34.43057933112932 38.0 33.0 40.0 26.0 40.0 36 34.55333847012248 38.0 34.0 40.0 26.0 40.0 37 34.33950254119237 38.0 34.0 40.0 26.0 40.0 38 34.16284507266786 38.0 33.0 39.0 25.0 40.0 39 34.039649433118626 38.0 33.0 39.0 25.0 40.0 40 33.73399276547774 38.0 33.0 39.0 23.0 40.0 41 33.272235907674435 37.0 33.0 39.0 21.0 40.0 42 33.151735184601776 37.0 33.0 39.0 20.0 40.0 43 32.89124683537097 37.0 32.0 39.0 18.0 40.0 44 32.81069540232066 37.0 32.0 39.0 18.0 40.0 45 32.4350430237722 36.0 32.0 39.0 14.0 40.0 46 31.701471576222485 36.0 31.0 39.0 2.0 40.0 47 31.45213486728485 36.0 31.0 39.0 2.0 40.0 48 31.2992192333532 36.0 31.0 39.0 2.0 40.0 49 31.039770894142922 36.0 30.0 39.0 2.0 40.0 50 30.670548358568126 35.0 30.0 39.0 2.0 40.0 51 30.017391700416383 35.0 30.0 39.0 2.0 40.0 52 29.77432541685803 35.0 29.0 39.0 2.0 40.0 53 29.498760717986478 35.0 29.0 39.0 2.0 40.0 54 29.12333228320156 35.0 29.0 38.0 2.0 40.0 55 28.661970173727223 35.0 28.0 38.0 2.0 40.0 56 16.369898162522443 20.0 2.0 23.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.26935780356871675 1 2 -0.6320047001060729 1 3 -0.520150699912783 1 4 -0.8059578561065379 1 5 -0.7282939964716633 1 6 -0.8106006310602325 1 7 -0.6518872192745491 1 8 -0.3703694453743225 1 9 -0.6695597592737244 1 10 -0.5377331949588751 1 11 -0.5906270866244441 1 12 -0.34698980115096845 1 13 -0.8237743405282032 1 14 -0.6510346626209937 1 15 -0.6670843175262888 1 16 -0.44956284493469667 1 17 -0.3263092755409005 1 18 -0.21340059037554227 1 19 -0.18145753792616404 1 20 -0.32966656695275987 1 21 -0.36224258470254256 1 22 -0.7075817589214779 1 23 -0.5539408869377525 1 24 -0.1816681734141028 1 25 -0.15446779352713946 1 26 -0.787447602035833 1 27 -0.5700893714380371 1 28 -0.6823107051765831 1 29 -0.6352586854075568 1 30 -0.7434776871236721 1 31 -0.8953228176393253 1 32 -0.6027484084863559 1 33 -0.5047131510737586 1 34 -0.6440448869774897 1 35 -0.5625414887947215 1 36 -0.28489464036466217 1 37 -0.0727071049434187 1 38 -0.19440011208527608 1 39 -0.10360935531667081 1 40 -0.13545421376500144 1 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49 0.8767668634380605 120 50 0.8715059902081634 120 51 0.6789612501966609 120 52 1.0711968998626489 120 53 0.9233364062117282 120 54 1.0249814717144794 120 55 1.5292324713280188 120 56 1.4872539876110693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 163.0 3 18.0 4 55.0 5 60.0 6 99.0 7 209.0 8 165.0 9 284.0 10 374.0 11 355.0 12 534.0 13 899.0 14 1136.0 15 906.0 16 1129.0 17 1261.0 18 1360.0 19 1384.0 20 1734.0 21 1860.0 22 1888.0 23 2200.0 24 2549.0 25 2828.0 26 3134.0 27 3725.0 28 4427.0 29 5064.0 30 6160.0 31 7322.0 32 8819.0 33 11200.0 34 14462.0 35 19749.0 36 28695.0 37 41281.0 38 50236.0 39 35735.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 43.57414246619019 44.57164112822109 3.8491757730804412 8.005040632508283 11 29.78679794579043 24.044728022197003 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28.667947073157773 26.435333146080207 21.310417599769224 25 23.540242976461656 30.65565679574791 23.44798785117692 22.356112376613517 26 24.6904527615147 29.409172248458436 25.375085009137294 20.525289980889568 27 22.080404301402133 24.76232093323707 26.40460538815215 26.752669377208647 28 21.824006445091317 27.617748322224166 27.56758605488968 22.990659177794836 29 24.917688745599296 25.590245831210602 27.120132610464363 22.37193281272574 30 22.492314482254823 24.957006757168077 29.590765203628173 22.959913556948923 31 25.96772965979146 24.780805236319356 28.38001369967273 20.871451404216455 32 25.04652906496561 25.23416774822162 30.113534724574574 19.605768462238192 33 25.083762297828272 24.540449575096705 29.359827601961562 21.01596052511346 34 21.96328221372767 27.015975511322964 31.823858184974796 19.196884089974567 35 20.65226498884073 28.77644137384694 31.62833921131009 18.942954426002238 36 21.229816884443565 28.635247838748533 30.358531416286766 19.776403860521143 37 23.473360968027066 26.90115517083727 30.49772928159956 19.127754579536102 38 22.09114341055714 28.837296200832736 29.45155337288575 19.620007015724372 39 23.543030192075914 26.70373930156987 29.84232687994874 19.910903626405478 40 22.033762456728255 27.674451445953736 29.383566087928948 20.908220009389062 41 21.028722380857854 27.567225030157736 30.88762998366138 20.516422605323033 42 20.966848824376292 25.841954319276255 30.19770030665124 22.993496549696214 43 20.02795877989718 26.09638903954716 30.873826456950354 23.001825723605307 44 21.253346675476557 25.314043242293575 30.516333304051912 22.91627677817796 45 22.152943333944616 26.316935631762334 30.16229598386209 21.367825050430955 46 21.644317096466093 28.093600764087874 30.03018147086915 20.231900668576884 47 20.99776761823214 27.329475084478833 31.155104662008227 20.517652635280808 48 22.16367314318054 26.125678274935087 30.32553372924068 21.385114852643696 49 21.942186192108792 26.954550840299195 29.903337881689236 21.19992508590277 50 20.919301976051795 26.80462442777869 30.559772985417677 21.716300610751833 51 20.105136390572135 26.524573241832815 31.70072448530958 21.669565882285475 52 20.401246963872474 27.14403197735575 31.151146556505445 21.303574502266336 53 20.3302882849798 26.281521606071735 31.912565797527236 21.475624311421228 54 20.3840856924254 27.22532517214996 30.78921193573068 21.601377199693957 55 20.557739341445025 27.748412555641888 29.50139511384818 22.192452989064908 56 20.840333561677912 28.578153164358344 28.520748102762717 22.06076517120103 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 240.5 11 481.0 12 1647.5 13 3498.5 14 4183.0 15 3342.0 16 2501.0 17 2185.5 18 1870.0 19 1797.5 20 1725.0 21 1685.0 22 1823.0 23 2001.0 24 2357.5 25 2714.0 26 3386.5 27 4059.0 28 4583.5 29 5569.5 30 6031.0 31 6232.0 32 6433.0 33 6446.5 34 6460.0 35 7281.5 36 8103.0 37 7881.5 38 7552.0 39 7444.0 40 8345.5 41 9247.0 42 8803.5 43 8360.0 44 8950.0 45 9540.0 46 10172.0 47 10880.0 48 10956.0 49 11290.5 50 11625.0 51 12465.0 52 13305.0 53 14178.5 54 14787.5 55 14523.0 56 14609.5 57 14696.0 58 15227.5 59 15759.0 60 15676.5 61 15594.0 62 14721.5 63 11475.0 64 9101.0 65 7513.5 66 5926.0 67 5251.0 68 4576.0 69 3768.0 70 2960.0 71 2629.5 72 1939.5 73 1580.0 74 1232.0 75 884.0 76 752.0 77 620.0 78 475.5 79 302.5 80 274.0 81 233.0 82 192.0 83 147.5 84 103.0 85 77.0 86 51.0 87 30.0 88 8.5 89 8.0 90 5.0 91 2.0 92 1.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0022773942055500096 23 0.003036525607400013 24 3.7956570092500164E-4 25 0.022394376354575096 26 0.0956505566331004 27 0.10969448756732547 28 0.11880406438952551 29 0.1643519485005257 30 0.237987694479976 31 0.2577251109280761 32 0.27328730466600115 33 0.3078277834501763 34 0.30630952064647626 35 0.34047043372972646 36 0.42169749372767673 37 0.3746313468129766 38 0.45206274980167693 39 0.482428005875677 40 0.5511293977431023 41 0.5704872484902774 42 0.6065459900781526 43 0.6243855780216276 44 0.6194512239096026 45 0.6509551770863777 46 0.6486777828808277 47 0.7040943752158779 48 0.7401531168037532 49 0.6930869698890529 50 0.7507809564296533 51 0.718897437551953 52 0.7682409786722032 53 0.7784892525971784 54 0.7815257782045784 55 0.8312488850257536 56 0.8191027825961535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 263459.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.281060810220943 #Duplication Level Percentage of deduplicated Percentage of total 1 61.906615308217795 17.507847520866623 2 11.071145767625387 6.262074933860677 3 6.156303265377337 5.223203610428947 4 4.180703002321867 4.72938863352552 5 3.0237957830597644 4.275807620920143 6 2.4413158141969427 4.142580059895468 7 1.9084942758592922 3.7781969870074663 8 1.5058583526822262 3.4069817315028144 9 1.230723805177898 3.1325557297340385 >10 6.062354883302688 28.750204016564247 >50 0.32613509777342337 6.3106593435790765 >100 0.17850192594183253 9.415886342846514 >500 0.0013421197439235529 0.26076163653547607 >1k 0.006710598719617765 2.803851832732987 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACCTGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1864 0.707510466524203 No Hit ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1447 0.5492315692384773 No Hit ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1425 0.5408811238181273 No Hit ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1417 0.5378445982107273 No Hit ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1234 0.468384074941452 No Hit ACCTGAGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGA 687 0.26076163653547607 No Hit ACCTGAGGGATTTGGGCGTATGGAATCATGGGGTTGTTCTACTTTTAGTCTCGGAG 490 0.1859871934532508 No Hit ACCTGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 437 0.1658702113042257 No Hit ACCTGAGGGTGCAGTCTCAGTCTTTATTTATTTTCCCAGCCACCATCCCCATAGAA 426 0.16169498859405068 No Hit ACCTGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 418 0.15865846298665068 No Hit ACCTGAGGGGTGTAGTATGTATGCAAAGTATAGTTATCAGCCTTGAGGGTTATAAA 400 0.15182628037000065 No Hit ACCTGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG 378 0.1434758349496506 No Hit ACCTGAGGGGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCCAACGATGTTG 376 0.14271670354780058 No Hit ACCTGAGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 375 0.1423371378468756 No Hit ACCTGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 360 0.13664365233300058 No Hit ACCTGAGGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 360 0.13664365233300058 No Hit ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 359 0.1362640866320756 No Hit ACCTGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 334 0.12677494410895052 No Hit ACCTGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 320 0.1214610242960005 No Hit ACCTGAGGGATCTTTCTTAGAACAAATGCAGTTCAATTGCATCCCATTTCCAGTCA 311 0.11804493298767552 No Hit ACCTGAGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 308 0.1169062358849005 No Hit ACCTGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 291 0.11045361896917547 No Hit ACCTGAGGGGTTATAGATCCTTCTAGATCTGATGTTCTTATATGAAATGGCTTGCA 280 0.10627839625900046 No Hit ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 277 0.10513969915622544 No Hit ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 275 0.10438056775437544 No Hit ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 274 0.10400100205345045 No Hit ACCTGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 268 0.10172360784790042 No Hit ACCTGAGGGACTCTTCTAGTTCCTGGGCCTATCTGATCTCTCTCTTTCTTCTGAAA 268 0.10172360784790042 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0022773942055500096 0.0 0.0 0.0 0.0 11 0.0034160913083250144 0.0 0.0 0.0 0.0 12 0.004175222710175018 0.0 0.0 0.0 0.0 13 0.004175222710175018 0.0 0.0 0.0 0.0 14 0.007211748317575031 0.0 0.0 0.0 0.0 15 0.007591314018500032 0.0 0.0 0.0 0.0 16 0.010248273924975043 0.0 0.0 0.0 0.0 17 0.010248273924975043 0.0 0.0 0.0 0.0 18 0.010627839625900045 0.0 0.0 0.0 0.0 19 0.010627839625900045 0.0 0.0 0.0 0.0 20 0.012905233831450055 0.0 0.0 0.0 0.0 21 0.012905233831450055 0.0 0.0 0.0 0.0 22 0.014423496635150062 0.0 0.0 0.0 0.0 23 0.015941759438850067 0.0 0.0 0.0 0.0 24 0.01935785074717508 0.0 0.0 0.0 0.0 25 0.02087611355087509 0.0 0.0 0.0 0.0 26 0.0231535077564251 0.0 0.0 0.0 0.0 27 0.02543090196197511 0.0 0.0 0.0 0.0 28 0.03340178168140014 0.0 0.0 0.0 0.0 29 0.034160913083250144 0.0 0.0 0.0 0.0 30 0.03909526719527517 0.0 0.0 0.0 0.0 31 0.03985439859712517 0.0 0.0 0.0 0.0 32 0.04175222710175018 0.0 0.0 0.0 0.0 33 0.04327048990545018 0.0 0.0 0.0 0.0 34 0.04478875270915019 0.0 0.0 0.0 0.0 35 0.05389832953135023 0.0 0.0 0.0 0.0 36 0.05617572373690024 0.0 0.0 0.0 0.0 37 0.057314420839675244 0.0 0.0 0.0 0.0 38 0.05845311794245025 0.0 0.0 0.0 0.0 39 0.05921224934430025 0.0 0.0 0.0 0.0 40 0.06148964354985026 0.0 0.0 0.0 0.0 41 0.06262834065262526 0.0 0.0 0.0 0.0 42 0.06376703775540027 0.0 0.0 0.0 0.0 43 0.06566486626002528 0.0 0.0 0.0 0.0 44 0.06718312906372528 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCACC 25 2.0038953E-5 50.32835 50 AGTCTCG 50 7.2759576E-12 50.28979 47 CAACGAT 95 0.0 50.260914 46 TCTCGAC 15 0.008252241 50.260914 46 CATTTCG 15 0.008252241 50.260914 46 CGATACA 15 0.008252241 50.260914 46 ACGATAC 15 0.008258488 50.251293 45 TGCGAAA 15 0.008258488 50.251293 44 TCCAACG 95 0.0 50.251293 44 CCAACGA 95 0.0 50.251293 45 GTCGATG 25 2.0221487E-5 50.25129 44 CTCGAAT 20 4.075845E-4 50.25129 45 TCGCGAC 15 0.008270992 50.232067 43 GTCGCGA 20 4.0835512E-4 50.232063 42 CGTCGCG 15 0.008302315 50.18406 41 TCGAATG 15 0.008327438 50.14572 40 TACGTAC 20 4.1183722E-4 50.14572 40 CTACGTA 20 4.1222558E-4 50.136147 39 CGGTATT 45 1.3278623E-10 50.126575 38 GCGGTAT 45 1.3278623E-10 50.11701 37 >>END_MODULE