FastQCFastQC Report
Tue 31 May 2016
SRR212926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423755
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT23190.5472501799388798No Hit
AACCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20700.4884898113296598No Hit
AACCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15200.3586978324739531No Hit
AACCTAGGGATTGAAGGAGCTTCAGATGACATGACTGTGGTAGATGCAGCTTCATTAA9890.2333895765241708No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA8880.20955504949794101No Hit
AACCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC7880.18595650788781254No Hit
AACCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA7460.17604512041155856No Hit
AACCTAGGGATTGAAATTCAAATTACTCTGAGAAAATGTTTAAATTTTTTAAGTATGA7310.1725053391700393No Hit
AACCTAGGGATCCTGAACGAGCCTTCTGCGGATGCCCCAGCTGCTCGCTACCAGACAA6750.15929015586836734No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6650.15693030170735447No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA6490.15315453504973392No Hit
AACCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA6430.15173862255312623No Hit
AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG6380.1505586954726198No Hit
AACCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA5780.1363995705065427No Hit
AACCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT5680.13403971634552986No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5620.13262380384892214No Hit
AACCTAGGGCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATTCAGATTGCC4920.1161048247218322No Hit
AACCTAGGGATTTAAAAATATTTTGCCCCATCCCCAAACCTGTCCAGCACTTATGTAA4820.11374497056081934No Hit
AACCTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT4560.10760934974218593No Hit
AACCTAGGGACCCGTTGAGCGTCGACAGCCCTCGCCCCAACCTTCACCAAGGGATCTA4470.10548548099727437No Hit
AACCTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4360.10288964142016023No Hit
AACCTAGGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4280.10100175809134995No Hit
AACCTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4270.10076577267524867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAA150.0071939752.04205741
GCACGGT150.0071939752.04205747
TACGTAC251.651279E-552.01133740
CGCGATT251.6559005E-551.98678630
ACCGCGA251.6570575E-551.98064828
CGACAGC550.051.98064823
TCGATAC251.6570575E-551.98064813
GACCCGT650.051.9806489
GAGCGTA150.007227637351.9806489
CGCTAAT150.007227637351.98064818
AACGCGA150.007227637351.98064823
CGTCGAA203.4515158E-451.98064828
ATAGTCG150.007227637351.98064827
GATTCGA203.4515158E-451.9806489
TCGACAG600.051.98064822
TAGGGGC50400.051.103995
TAGGGAG62850.050.905475
CTAGGGA215500.050.871094
CCTAGGG433400.050.8232573
CTAGGGG170600.050.822824
Produced by FastQC (version 0.11.5)