FastQCFastQC Report
Tue 31 May 2016
SRR212925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424428
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT23000.54190581205764No Hit
AACCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20020.4716936677127805No Hit
AACCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14780.3482333870526921No Hit
AACCTAGGGATTGAAGGAGCTTCAGATGACATGACTGTGGTAGATGCAGCTTCATTAA9800.23089899818108137No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA8370.1972065933444542No Hit
AACCTAGGGATTGAAATTCAAATTACTCTGAGAAAATGTTTAAATTTTTTAAGTATGA7410.17458791597161355No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA7340.17293863741317728No Hit
AACCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC7250.17081813640947346No Hit
AACCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA7110.16751957929260086No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6810.16045124261358817No Hit
AACCTAGGGATCCTGAACGAGCCTTCTGCGGATGCCCCAGCTGCTCGCTACCAGACAA6690.15762390794198308No Hit
AACCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA6320.14890629270453412No Hit
AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG6140.1446652906971265No Hit
AACCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT5720.13476961934650872No Hit
AACCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA5270.12416711432798969No Hit
AACCTAGGGCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATTCAGATTGCC5000.11780561131687825No Hit
AACCTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.1163919439810757No Hit
AACCTAGGGATTTAAAAATATTTTGCCCCATCCCCAAACCTGTCCAGCACTTATGTAA4700.11073727463786553No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA4640.10932360730206302No Hit
AACCTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT4540.10696749507572545No Hit
AACCTAGGGACCCGTTGAGCGTCGACAGCCCTCGCCCCAACCTTCACCAAGGGATCTA4530.10673188385309168No Hit
AACCTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.10626066140782418No Hit
AACCTAGGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4430.10437577162675414No Hit
AACCTAGGGCATAGCAGATCACTATGTGGAAGTTCTGGAGTGTAAGATTCGTTGTGAG4260.10037038084198026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACA150.00715050852.1218724
CGTACAG150.00715385152.11570725
ATGAGCG307.8680205E-752.1095522
CGTAAGG150.007157195452.1095522
ATTCGTT203.4375128E-452.0234847
CGATTAT150.007204138752.02347648
CGGTATA150.007210863852.01120842
TATAGGG203.4475361E-451.99280539
TACCGTT150.007224327451.9866837
TGCGATC251.6582364E-551.97442232
CTATGCG150.007237809751.96217315
GAATCGC150.007237809751.96217317
TACGGTA150.007237809751.96217316
AATACGA150.007237809751.96217312
ATCGCGC150.007241183451.9560519
GCGCATA150.00724455851.9499329
TAGGGGC50450.051.1261445
CTAGGGA213300.050.9270064
TAGGGAT72750.050.91455
CCTAGGG433050.050.9122543
Produced by FastQC (version 0.11.5)