FastQCFastQC Report
Tue 31 May 2016
SRR212924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421247
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT20340.482852103397769No Hit
AACCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17940.42587840388180803No Hit
AACCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13040.30955710070338777No Hit
AACCTAGGGATTGAAGGAGCTTCAGATGACATGACTGTGGTAGATGCAGCTTCATTAA9010.2138887635995034No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA7700.18279061928037468No Hit
AACCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA6680.1585767969860913No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA6440.1528794270344952No Hit
AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG6310.1497933516440473No Hit
AACCTAGGGATTGAAATTCAAATTACTCTGAGAAAATGTTTAAATTTTTTAAGTATGA6300.1495559612293975No Hit
AACCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC6270.14884378998544798No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6260.14860639957079813No Hit
AACCTAGGGATCCTGAACGAGCCTTCTGCGGATGCCCCAGCTGCTCGCTACCAGACAA5890.13982295422875415No Hit
AACCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5540.13151428971600984No Hit
AACCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA5070.1203569402274675No Hit
AACCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT4720.1120482757147232No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA4540.1077752482510261No Hit
AACCTAGGGCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATTCAGATTGCC4540.1077752482510261No Hit
AACCTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.1070630770070766No Hit
AACCTAGGGATTTAAAAATATTTTGCCCCATCCCCAAACCTGTCCAGCACTTATGTAA4400.10445178244592841No Hit
AACCTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT4240.100653535811531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG307.62675E-752.345048
GCGTAAT150.007030652452.345049
CTTCGTA203.4403577E-452.01464536
CGAAATA150.007215722552.00226225
CGTCCTA150.007215722552.00226224
AATTACG251.652961E-552.0022643
CGTTATT150.007219120451.9960733
CTACCGT150.007222519751.98988341
CGGAATA150.007225920451.98369228
CTCGATC150.007242940451.95277446
ATAACCG150.007242940451.95277447
CACGTTC150.007242940451.95277430
GCGTTAT150.007263404351.91572632
CAATCGA150.007294180351.86024518
CGAAACC251.6811175E-551.8540913
AATACGA150.00729760651.8540912
TAGGGAT70450.051.3419725
CTAGGGA209200.051.1695144
CTAGGGG170400.051.076444
TAGGGAG60750.051.052575
Produced by FastQC (version 0.11.5)