FastQCFastQC Report
Tue 31 May 2016
SRR212923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392429
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT22490.5730972991292692No Hit
AACCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19290.49155388618068496No Hit
AACCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14020.35726207798098514No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11720.2986527499241901No Hit
AACCTAGGGATTGAAGGAGCTTCAGATGACATGACTGTGGTAGATGCAGCTTCATT9010.22959567208335774No Hit
AACCTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT7860.20029100805496028No Hit
AACCTAGGGATTGAAATTCAAATTACTCTGAGAAAATGTTTAAATTTTTTAAGTAT6830.17404422201213468No Hit
AACCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA6300.1605385942425254No Hit
AACCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC5950.151619783451274No Hit
AACCTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5510.14040756417084366No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5420.1381141556816647No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA5300.1350562776960928No Hit
AACCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC5180.13199839971052088No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4900.12486335107751977No Hit
AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA4830.12307958891926947No Hit
AACCTAGGGATCCTGAACGAGCCTTCTGCGGATGCCCCAGCTGCTCGCTACCAGAC4700.11976688776823324No Hit
AACCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG4600.11721865611358996No Hit
AACCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4520.11518007078987537No Hit
AACCTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.11492524762441104No Hit
AACCTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.11365113179708941No Hit
AACCTAGGGATTTAAAAATATTTTGCCCCATCCCCAAACCTGTCCAGCACTTATGT4410.11237701596976779No Hit
AACCTAGGGAGCAGACACGGGACAACTCTATGGGAGAATATTTTGCCTACAACCTT4330.11033843064605317No Hit
AACCTAGGGCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATTCAGATTG4230.1077901989914099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT150.0082295850.30053748
ATTCGTT402.5502231E-950.2682247
GAGTAGC150.00825051950.26821547
TACGGTT150.00826310150.2488446
TCGAATG150.00826310150.2488446
GCGTACA150.00826310150.2488446
AACACGT252.0276571E-550.2359444
ACACGTA150.00827149750.23593545
GTCGAAT150.00827149750.23593545
ATTACGG150.00827149750.23593544
ATGCGTT402.6284397E-950.10080733
TATGCGT355.2048563E-850.08797532
CGAAATA150.00847491249.9281325
TAAAACG204.2368477E-449.8644821
CGATTCG150.00853493549.83906613
CCGTAGA204.2475195E-449.83906613
GAACGTA204.2475195E-449.8390669
TCCGTAG204.2475195E-449.83906612
GCCGTTG204.2475195E-449.83906614
AATACGA402.752131E-949.83906612
Produced by FastQC (version 0.11.5)