FastQCFastQC Report
Tue 31 May 2016
SRR212922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359871
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT19780.5496413992791861No Hit
AACCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16690.46377729797621925No Hit
AACCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12890.35818390478810463No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10250.28482428425741446No Hit
AACCTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT9070.2520347568989999No Hit
AACCTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC7440.20674074876830864No Hit
AACCTAGGGATTGAAGGAGCTTCAGATGACATGACTGTGGTAGATGCAGCTTCATT6850.1903459850891014No Hit
AACCTAGGGATTGAAATTCAAATTACTCTGAGAAAATGTTTAAATTTTTTAAGTAT6390.1775636269663296No Hit
AACCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC6200.17228395730692386No Hit
AACCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA5770.1603352312356372No Hit
AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA5110.14199532610296467No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5030.13977230729900436No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA5030.13977230729900436No Hit
AACCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC5000.13893867524751924No Hit
AACCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4780.1328253735366284No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4750.13199174148514328No Hit
AACCTAGGGATCCTGAACGAGCCTTCTGCGGATGCCCCAGCTGCTCGCTACCAGAC4330.12032089276435168No Hit
AACCTAGGGATTTAAAAATATTTTGCCCCATCCCCAAACCTGTCCAGCACTTATGT4260.11837575131088642No Hit
AACCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG4110.11420759105346082No Hit
AACCTAGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC4050.11254032695049059No Hit
AACCTAGGGAGCAGACACGGGACAACTCTATGGGAGAATATTTTGCCTACAACCTT3880.10781641199207492No Hit
AACCTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3750.10420400643563943No Hit
AACCTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG3740.1039261290851444No Hit
AACCTAGGGCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATTCAGATTG3650.10142523293068906No Hit
AACCTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.10003584617821386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA150.00828019550.22172549
ACGCAAT355.106267E-850.20767247
CGTACTG204.1038767E-450.186642
ACGTACT204.115221E-450.1585441
TACGTAC204.118061E-450.15152440
CGCTCTA150.00834452650.12349737
AGTAGTA150.0083722150.0815234
GGTAATC150.00839533350.0465931
TATCGTT150.00839533350.0465931
TTTATCG150.00840459550.0326329
AGCCGTT800.050.0186828
CGTGATA150.00842314250.00473826
TCGCACT150.00842778349.9977725
ATATTGG204.2155513E-449.91430321
CGCTAGA150.00852101449.8588117
GAATCCG204.241662E-449.8518879
GGTCGTC150.00852569649.8518839
AACGGTG150.00852569649.85188313
CTCGCGT150.00852569649.85188311
ATAGTGC150.00852569649.85188313
Produced by FastQC (version 0.11.5)