FastQCFastQC Report
Tue 31 May 2016
SRR212921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356442
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA53811.509642522486127No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA35270.989501798329041No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG29890.8385656011356687No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA17250.48394970289696504No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC14910.4183008736344202No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA12720.35686030265793595No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC11940.33497735957042096No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9250.25950926097373483No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT9220.2586676093165227No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTG8460.2373457673338159No Hit
CTGAAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA7220.20255749883571522No Hit
CTGAAAGGGACCAAGGGATCTACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGA6840.1918965778443618No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA6620.18572446569147294No Hit
CTGAAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG5950.16692757868040242No Hit
CTGAAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5800.16271932039434187No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG5650.15851106210828128No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5650.15851106210828128No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTGA5630.15794996100347322No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG5560.15598610713664496No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5360.1503750960885642No Hit
CTGAAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC5190.14560573669769555No Hit
CTGAAAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT4960.1391530739924027No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4850.1360670179159583No Hit
CTGAAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT4820.13522536625874615No Hit
CTGAAAGGGAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCA4810.13494481570634215No Hit
CTGAAAGGGGAGTGCAGTGCTCTGAAAGGGGTACATCAAAGGATGTTTTCCTGAGGCA4760.13354206294432194No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA4730.13270041128710983No Hit
CTGAAAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC4480.1256866474770089No Hit
CTGAAAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA4460.1251255463722008No Hit
CTGAAAGGGATGTAGATTTTTATCAGAATAATCTCTGAAATATCTATCCCCAAGACCC4450.12484499581979676No Hit
CTGAAAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG4360.12232004084816042No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA4160.11670902980007967No Hit
CTGAAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4150.11642847924767563No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC4120.11558682759046353No Hit
CTGAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA4040.11334242317123122No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA4000.11222022096161507No Hit
CTGAAAGGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG3940.11053691764719084No Hit
CTGAAAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCTTCCAG3800.10660920991353431No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG3760.10548700770391817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA150.007179008352.06774551
CGTCGTC251.6401853E-552.0677451
CGTACGT150.00718352.0604350
TCGTACG150.007186992652.05311649
AATTTCG150.007194983352.0384945
CTGGTCG251.6456515E-552.03848645
TCGTCTA251.6511329E-552.00926639
TCGCCAA150.00721498952.0019734
TACCCAT203.44379E-452.0019725
ATCGGTT150.00721498952.0019737
CCGGTAA251.6525057E-552.00196535
TATCGTC251.6525057E-552.00196537
CCGTTAA251.6525057E-552.00196530
GCAAATC150.00721899551.99466732
GACGATA150.00721899551.99466733
TATACCC150.00722300351.9873723
CGACCTA150.007227012451.9800820
TCTGACG150.007231023651.97278614
CGCATGT150.007231023651.97278615
GAGGTAT150.007231023651.9727869
Produced by FastQC (version 0.11.5)