FastQCFastQC Report
Tue 31 May 2016
SRR212920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367039
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA53451.4562485185498No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA34870.9500352823541912No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG29500.8037293039704227No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA16890.46016908284950647No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC15050.41003817033067336No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA13120.3574552023082016No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC11960.32585093137241544No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9070.24711270464446558No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT9020.2457504515868886No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTG8320.22667890878081076No Hit
CTGAAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA6980.1901705268377475No Hit
CTGAAAGGGACCAAGGGATCTACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGA6650.18117965665773936No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6610.18008985421167778No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA6320.17218878647773125No Hit
CTGAAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG6000.16347036690923855No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG5890.16047341018256917No Hit
CTGAAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5860.15965605834802296No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTGA5810.15829380529044598No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG5600.1525723424486226No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5600.1525723424486226No Hit
CTGAAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC5540.15093763877953023No Hit
CTGAAAGGGGAGTGCAGTGCTCTGAAAGGGGTACATCAAAGGATGTTTTCCTGAGGCA5490.14957538572195325No Hit
CTGAAAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA5300.1443988241031607No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC5270.1435814722686145No Hit
CTGAAAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT5110.13922226248436814No Hit
CTGAAAGGGAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCA4690.12777933680072145No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA4670.12723443557769065No Hit
CTGAAAGGGATGTAGATTTTTATCAGAATAATCTCTGAAATATCTATCCCCAAGACCC4660.12696198496617528No Hit
CTGAAAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC4540.12369257762799048No Hit
CTGAAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT4480.1220578739588981No Hit
CTGAAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4450.12124052212435191No Hit
CTGAAAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG4360.11878846662071332No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA4130.11252210255585919No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA4130.11252210255585919No Hit
CTGAAAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCTTCCAG3960.10789044216009742No Hit
CTGAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3940.10734554093706664No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG3930.10707309032555122No Hit
CTGAAAGGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG3870.10543838665645884No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC3760.10244142992978948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACCG203.409167E-452.1089123
TACCGCG150.007203299652.02359852
ATTAGCG203.439174E-452.01650250
CTAACGG150.00720718752.01650242
ATCCCGC251.6498687E-552.01650244
CGTCAAG150.007218857851.99522439
TTACTAG203.448449E-451.9881435
ACCGGTA150.00722275151.98813634
CCGGTAA150.00722275151.98813635
GGCGTAG150.007230542651.9739729
TACGGAC150.007230542651.9739730
ACGGACA203.453094E-451.9739731
CCCGTAC150.00723444151.96688516
TTGACGC203.4554183E-451.96688512
TCCGTTC251.6592163E-551.96688516
CATCGAC150.00723444151.96688516
CGACTCG150.00723444151.96688514
ATGGTCG251.6618953E-551.95272419
GAAAGGG369250.051.6361543
TGAAAGG369300.051.6291662
Produced by FastQC (version 0.11.5)