FastQCFastQC Report
Tue 31 May 2016
SRR212919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371614
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA49731.3382165365136942No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA32800.8826362838859677No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG28950.779034159100572No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA16880.45423477048765654No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC14270.38400060277599873No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA11680.31430462792036895No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC10650.2865876958349255No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7930.21339346741511353No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT7840.2109715995629874No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTG7810.21016431027894533No Hit
CTGAAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA6360.17114532821691325No Hit
CTGAAAGGGACCAAGGGATCTACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGA6060.16307243537649282No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA6050.16280333894847882No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5800.15607592824812844No Hit
CTGAAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5720.1539231568240163No Hit
CTGAAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG5490.14773393897969397No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG5420.14585026398359588No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTGA5060.13616279257509137No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG4930.13266453901090916No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4870.13104996044282508No Hit
CTGAAAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA4840.13024267115878302No Hit
CTGAAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC4810.129435381874741No Hit
CTGAAAGGGAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCA4750.1278208033066569No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA4680.1259371283105588No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA4590.12351526045843268No Hit
CTGAAAGGGGAGTGCAGTGCTCTGAAAGGGGTACATCAAAGGATGTTTTCCTGAGGCA4570.12297706760240465No Hit
CTGAAAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT4370.11759513904212435No Hit
CTGAAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT4280.11517327118999822No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG4260.11463507833397019No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA4120.110867728341774No Hit
CTGAAAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC4100.11032953548574596No Hit
CTGAAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4060.10925314977368991No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC4030.10844586048964786No Hit
CTGAAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG3960.10656218549354975No Hit
CTGAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3930.10575489620950772No Hit
CTGAAAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG3760.10118025693326947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA251.5889384E-552.3483739
GGACGTA503.6379788E-1252.3483738
GGTCGAC203.332901E-452.348379
GACGTAC203.332901E-452.348379
GGATAGT650.052.348378
ACGTACA203.332901E-452.3483710
GACGAAT150.007028228652.348379
TACGAAC203.430159E-452.04430839
TATCCGA150.007192031552.04430439
CCTGTCG150.00719587452.03727738
CTATGCG150.007203564552.02322424
TAGCGTG150.007203564552.02322426
CAACCGG150.007203564552.02322424
TATGCGT150.007203564552.02322425
GAAACGC150.007203564552.02322429
TATAGCG150.007203564552.02322424
TCCGCGT150.00721126152.0091842
CGTCTAG150.00721126152.0091841
GAATACG150.00721126152.0091850
GCGTCTA150.00721126152.0091840
Produced by FastQC (version 0.11.5)