FastQCFastQC Report
Tue 31 May 2016
SRR212918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408876
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC95122.3263776792964124No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC56111.3722986920239877No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT21130.5167825942339487No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC18580.45441649791134725No Hit
CTGAAAGGGATGTAGATTTTTATCAGAATAATCTCTGAAATATCTATCCCCAAGAC14260.3487609935530577No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC11040.27000851113785107No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA10570.258513583580352No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9550.23356714505131138No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA7880.1927234662831763No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCA6890.16851074653440162No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6850.16753245482738044No Hit
CTGAAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6420.15701581897690253No Hit
CTGAAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC6270.15334722507557302No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCT6190.15139064166153063No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT5850.14307516215185045No Hit
CTGAAAGGGACCAAGGGATCTACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGC5780.14136315166456334No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC5740.14038485995754216No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG5720.13989571410403154No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC5660.13842827654349973No Hit
CTGAAAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG5260.12864535947328776No Hit
CTGAAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC5210.12742249483951124No Hit
CTGAAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC5190.12693334898600064No Hit
CTGAAAGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATC5150.12595505727897943No Hit
CTGAAAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5100.12473219264520295No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCC4900.11984073411009696No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC4830.11812872362280985No Hit
CTGAAAGGGAAGGATGGCTAGAGCTGGCCTATAACTTTCTTTTAGAGTTGATAATC4830.11812872362280985No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA4820.11788415069605455No Hit
CTGAAAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG4780.11690585898903336No Hit
CTGAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC4730.11568299435525686No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4700.11494927557499095No Hit
CTGAAAGGGGAGTGCAGTGCTCTGAAAGGGGTACATCAAAGGATGTTTTCCTGAGG4490.10981324411312966No Hit
CTGAAAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG4410.10785666069908725No Hit
CTGAAAGGGATTGTCTTTCGATTTGAACCTTTTCTGGAGAAAACATAGAGGGCTCT4330.10590007728504486No Hit
CTGAAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA4280.10467721265126836No Hit
CTGAAAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG4270.10443263972451305No Hit
CTGAAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG4160.10174233753020476No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC4130.10100861874993886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTA150.0083057550.1838344
CGAACCG150.0083057550.1838345
AGCGATA150.0083057550.1838344
GCGATAC150.0083057550.1838345
GCGTGTC150.00830979150.17765842
CGAACTC252.044761E-550.16531441
CGTCTAT355.17266E-850.1283240
ACGAACT252.0537054E-550.1283240
CGACACG204.1381744E-450.10368738
AATTGCG150.00835839350.10368738
TTATCGT355.1977622E-850.0975336
CCGGTAA301.0374933E-650.0606435
TGTTGCG204.1683664E-450.02993432
TCTCGGA252.0777163E-550.0299332
GTACGTC252.0777163E-550.0299331
CCGTTAA355.2583346E-850.02379630
TACGGAC204.1708903E-450.02379230
GTCGAAC150.00841536350.01766229
ACGCTAG150.00841536350.01766227
CGTCGAA150.00841536350.01766228
Produced by FastQC (version 0.11.5)