SRR212917_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212917_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	371187
Sequences flagged as poor quality	0
Sequence length	56
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	8564	2.307192870439967	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	5101	1.3742399383599102	No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	2015	0.5428530632807722	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	1745	0.47011344686101614	No Hit
CTGAAAGGGATGTAGATTTTTATCAGAATAATCTCTGAAATATCTATCCCCAAGAC	1193	0.32140134218062594	No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	1047	0.28206806811660967	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC	942	0.25378043950892676	No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	880	0.23707726833105686	No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	766	0.2063649858427154	No Hit
CTGAAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	722	0.19451112242616256	No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	637	0.17161161355327637	No Hit
CTGAAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	617	0.16622349381847965	No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCA	613	0.16514586987152027	No Hit
CTGAAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	591	0.15921893816324387	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC	535	0.14413220290581297	No Hit
CTGAAAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG	529	0.14251576698537397	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCT	521	0.14036051909145525	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	519	0.13982170711797556	No Hit
CTGAAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	511	0.13766645922405688	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCC	500	0.13470299336991864	No Hit
CTGAAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	497	0.13389477540969916	No Hit
CTGAAAGGGACCAAGGGATCTACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGC	467	0.12581259580750404	No Hit
CTGAAAGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATC	466	0.1255431898207642	No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA	462	0.12446556587380483	No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG	461	0.124196159887065	No Hit
CTGAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	461	0.124196159887065	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	457	0.12311853594010565	No Hit
CTGAAAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	444	0.11961625811248777	No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	438	0.11799982219204874	No Hit
CTGAAAGGGGAGTGCAGTGCTCTGAAAGGGGTACATCAAAGGATGTTTTCCTGAGG	436	0.11746101021856908	No Hit
CTGAAAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	432	0.11638338627160973	No Hit
CTGAAAGGGAAGGATGGCTAGAGCTGGCCTATAACTTTCTTTTAGAGTTGATAATC	396	0.10668477074897559	No Hit
CTGAAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	394	0.10614595877549592	No Hit
CTGAAAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG	388	0.10452952285505687	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC	387	0.10426011686831704	No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTC	385	0.10372130489483737	No Hit
CTGAAAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	379	0.10210486897439836	No Hit
CTGAAAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA	376	0.10129665101417883	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC	374	0.10075783904069915	No Hit
CTGAAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG	373	0.10048843305395933	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCGT	15	0.008269082	50.239105	50
GTGTCGT	20	4.0829222E-4	50.2391	50
AGTGTCG	25	2.0299445E-5	50.225464	49
GCGATGA	20	4.088397E-4	50.22546	49
CGCGATG	20	4.0911365E-4	50.21865	48
CGTGGTA	20	4.104856E-4	50.184593	45
GCGTTAC	15	0.008304645	50.184593	44
CGTTACG	15	0.008304645	50.184593	45
ACTCGCA	20	4.1131052E-4	50.16418	41
CGTCTAT	15	0.008331393	50.143787	40
ACGCCGT	20	4.1241248E-4	50.13699	39
AACTCGC	20	4.1241248E-4	50.13699	39
AGTCACG	20	4.1268833E-4	50.1302	38
ACCGCAC	20	4.1268833E-4	50.1302	38
TTATCGT	15	0.008340323	50.1302	36
CGTAGTT	15	0.008340323	50.1302	38
TCGTAGT	15	0.008340323	50.1302	37
TATCGTC	15	0.008340323	50.1302	37
ACCGGTA	40	2.6157068E-9	50.123405	34
CCGACTC	15	0.00836268	50.096264	33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content