FastQCFastQC Report
Tue 31 May 2016
SRR212916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222922
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36121.6202976825975004No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24251.087824440835808No Hit
CAACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9190.41225181902190006No Hit
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9080.40731735764078913No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG7880.3534868698468523No Hit
CAACAAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7410.33240326212756033No Hit
CAACAAGGGCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACAC6700.300553556849481No Hit
CAACAAGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCGTCAGGAC6310.2830586483164515No Hit
CAACAAGGGAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGT6090.27318972555422977No Hit
CAACAAGGGATGTAAGAACGCATTTGGAGGACATGATAGCTTGCATAAAAGATGACGC5850.2624236279954424No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5170.23191968491221146No Hit
CAACAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4960.2224993495482725No Hit
CAACAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4950.22205076214998967No Hit
CAACAAGGGAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGC4350.19513551825302125No Hit
CAACAAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4050.181677896304537No Hit
CAACAAGGGAGTGTGGTATTTGTGTGTACCTATGTGTGGTATGTCTGACTATGTGTCT3600.16149146338181067No Hit
CAACAAGGGGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAGTGC3520.15790276419554822No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.15341689021272015No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3260.14623949184019522No Hit
CAACAAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC3140.14085644306080153No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3110.1395106808659531No Hit
CAACAAGGGCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGCCT3110.1395106808659531No Hit
CAACAAGGGGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGTCACCCAGACA2940.1318846950951454No Hit
CAACAAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2920.13098752029857977No Hit
CAACAAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.1148383739603987No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2520.11304402436726749No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG2340.10496945119817694No Hit
CAACAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA2310.10362368900332852No Hit
CAACAAGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2280.10227792680848011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA150.007169785452.0782252
CACCCGC150.007182542252.0548246
GCTATGC203.4237682E-452.05481747
CCTAGGT307.9204074E-752.0431348
CGTTTGG307.9204074E-752.0431348
CGATAGC150.007188927452.0431345
GGTCATT203.4275686E-452.04312545
CGTACTG203.4351792E-452.0197642
CGCGGCA251.6515338E-551.9964126
GCGAAGC150.007214508551.9964138
CCGTGCT251.6515338E-551.9964138
CGCGAAG150.007214508551.9964137
GCCGCGC150.007214508551.9964127
TATTCGT307.9696656E-751.9964136
AGACCCG150.007214508551.9964122
ACTACGT150.007214508551.9964123
AACGAAA150.007214508551.9964131
ATGCGCC251.6515338E-551.9964121
GCGTGTC150.007214508551.9964123
GGCGCAA150.007214508551.9964137
Produced by FastQC (version 0.11.5)