FastQCFastQC Report
Tue 31 May 2016
SRR212915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223929
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35851.6009538737724902No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24121.07712712511555No Hit
CAACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9530.42558132265137605No Hit
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9410.4202224812328908No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG7920.35368353362003135No Hit
CAACAAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7240.32331676558194783No Hit
CAACAAGGGCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACAC6980.311705942508563No Hit
CAACAAGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCGTCAGGAC6520.2911637170710359No Hit
CAACAAGGGAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGT5840.2607969490329524No Hit
CAACAAGGGATGTAAGAACGCATTTGGAGGACATGATAGCTTGCATAAAAGATGACGC5730.2558846777326742No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5250.23444931205873293No Hit
CAACAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.21613993721224137No Hit
CAACAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4740.2116742360301703No Hit
CAACAAGGGAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGC4440.19827713248395695No Hit
CAACAAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3570.15942553219993838No Hit
CAACAAGGGGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAGTGC3410.1522804103086246No Hit
CAACAAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC3400.1518338401904175No Hit
CAACAAGGGAGTGTGGTATTTGTGTGTACCTATGTGTGGTATGTCTGACTATGTGTCT3360.15004755971758907No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3270.14602842865372506No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.1344176055803402No Hit
CAACAAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.13218475498930463No Hit
CAACAAGGGGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGTCACCCAGACA2890.12905876416185488No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2860.12771905380723356No Hit
CAACAAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.11744794108847001No Hit
CAACAAGGGCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGCCT2430.10851653872432779No Hit
CAACAAGGGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2360.10539054789687805No Hit
CAACAAGGGGGTTTGGTCTGGGATCAATAGGGAAACACAGGTAGCCAACTAGGAGGAA2310.10315769730584248No Hit
CAACAAGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.10271112718763536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC150.007158672452.09872427
ACCGCGT150.007158672452.09872426
CGTCTCG150.007158672452.09872427
TGACGCG150.007158672452.09872425
CTGCCGA150.007158672452.09872424
CGGTGAA203.4095754E-452.09872425
TTCGAAC150.007158672452.09872427
TGCAATC150.007158672452.09872426
TACCGCG150.007158672452.09872425
AGTCCGT150.00717135852.0754123
ACCGGAT150.00717135852.0754122
CCGGATA150.00717135852.0754123
CGTAAAC150.00717135852.0754121
TATGACC150.00720951652.0055952
CCACGAC203.4398364E-452.0055952
GCGTCGG203.4398364E-452.0055950
ATGACGC750.052.00558552
AGCCGTT203.4436342E-451.9939728
CGAAATT251.6520165E-551.9939748
CCGTGCG150.007215890651.9939741
Produced by FastQC (version 0.11.5)