FastQCFastQC Report
Tue 31 May 2016
SRR212914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223887
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30021.3408549848807656No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20800.9290400961199177No Hit
CAACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8390.3747426156945245No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG8020.3582164216770067No Hit
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7440.3323104959198167No Hit
CAACAAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6320.28228525997489806No Hit
CAACAAGGGCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACAC6080.27156556655812975No Hit
CAACAAGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCGTCAGGAC5780.2581659497871694No Hit
CAACAAGGGAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGT5530.2469996024780358No Hit
CAACAAGGGATGTAAGAACGCATTTGGAGGACATGATAGCTTGCATAAAAGATGACGC5220.2331533318147101No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA4570.20412082881096266No Hit
CAACAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4500.20099425156440526No Hit
CAACAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4140.18491471143925284No Hit
CAACAAGGGAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGC3910.1746416719148499No Hit
CAACAAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3760.1679418635293697No Hit
CAACAAGGGGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAGTGC3400.1518623234042173No Hit
CAACAAGGGGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGTCACCCAGACA3040.13578278327906487No Hit
CAACAAGGGAGTGTGGTATTTGTGTGTACCTATGTGTGGTATGTCTGACTATGTGTCT3020.1348894754943342No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2970.13265620603250747No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.13131624435541142No Hit
CAACAAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2900.12952962878595006No Hit
CAACAAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC2890.1290829748935847No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2740.12238316650810455No Hit
CAACAAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2520.11255678087606694No Hit
CAACAAGGGCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGCCT2450.10943020362950953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG251.5779562E-552.3981979
GCGACGG150.00699817752.3981939
GATTTCG150.00699817752.3981939
GGATATA150.00699817752.3981938
GGATAGC401.7607817E-952.3981938
GGATACT307.557246E-752.3981938
GGGCGGT203.314398E-452.3981937
ATCCCTA150.00699817752.39819310
ATATGTC150.00716841552.08077239
GCGCTTG353.817513E-852.08077238
ATAGGCT251.648848E-552.01075436
ATCGTTC150.0072066652.01075437
ATATTCG203.4381298E-452.01075435
CTCGTTT203.441936E-451.99910734
GTAGGAA203.441936E-451.99910728
CCGATAA150.00721304951.99910427
TTTCGTA150.00721304951.99910425
AGACGTC150.00721304951.99910424
AACGCCA150.00721304951.99910433
CCATAAC150.00721304951.99910433
Produced by FastQC (version 0.11.5)