FastQCFastQC Report
Tue 31 May 2016
SRR212913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220507
Sequences flagged as poor quality0
Sequence length56
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34481.5636691805702312No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24501.1110758388622584No Hit
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10440.4734543574580399No Hit
CAACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8500.38547529103384476No Hit
CAACAAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7530.34148575782174717No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA6450.2925077208433292No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6260.2838912143378668No Hit
CAACAAGGGCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAAC5960.2702862040660841No Hit
CAACAAGGGATGTAAGAACGCATTTGGAGGACATGATAGCTTGCATAAAAGATGAC5750.26076269687583614No Hit
CAACAAGGGAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAA5440.24670418626166063No Hit
CAACAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5090.23083167427791407No Hit
CAACAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5050.22901767290834305No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4550.20634265578870514No Hit
CAACAAGGGAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGA3970.18003963592992514No Hit
CAACAAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3800.17233013010924822No Hit
CAACAAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG3240.14693411093525374No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.1419456071689334No Hit
CAACAAGGGAGTGTGGTATTTGTGTGTACCTATGTGTGGTATGTCTGACTATGTGT3040.1378641040873986No Hit
CAACAAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.13468960169064928No Hit
CAACAAGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCGTCAGG2910.1319685996362927No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2810.12743359621236516No Hit
CAACAAGGGGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGTCACCCAGA2810.12743359621236516No Hit
CAACAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2780.12607309518518686No Hit
CAACAAGGGCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC2610.11836358936450997No Hit
CAACAAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.11518908696776066No Hit
CAACAAGGGAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG2520.11428208628297513No Hit
CAACAAGGGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.11246808491340411No Hit
CAACAAGGGGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAGT2470.11201458457101136No Hit
CAACAAGGGATTAAGGAAATACCAGAATGACAGGACCATTGCAAGGGTATAATTTC2410.10929358251665479No Hit
CAACAAGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGT2240.1015840766959779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTC150.00828299550.2109150
AATCTTA150.00828299550.2109150
CGAGCGA204.0905838E-450.2109149
AATCGGG204.118311E-450.14223547
CGATTGC252.0499783E-550.1308144
GCGGGCC150.00833544350.1308143
CTCATAT204.122947E-450.1308143
TAGGCTT150.00833544350.1308145
CAATCGG204.122947E-450.1308146
CTAGCAT150.00833544350.1308142
GTCATAA150.00834295650.1193939
CAGCGTT150.00834295650.1193939
CGTGGGC150.00834295650.1193940
CACGGTT204.1275867E-450.1193940
TAACGGC150.00834295650.1193937
GCCATTC204.1275867E-450.1193936
TCTAGGT355.1652023E-850.1193938
TCTACGT204.1275867E-450.1193938
TATTCGT204.1275867E-450.1193936
CGGTCCT301.0269432E-650.1193938
Produced by FastQC (version 0.11.5)