FastQCFastQC Report
Tue 31 May 2016
SRR212912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193004
Sequences flagged as poor quality0
Sequence length56
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30101.5595531698824894No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21271.1020496984518455No Hit
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8540.4424778761061947No Hit
CAACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7810.4046548258067191No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA7210.3735673872044103No Hit
CAACAAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5920.30672939420944645No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5640.29222192286170234No Hit
CAACAAGGGCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAAC5500.28496818718783035No Hit
CAACAAGGGATGTAAGAACGCATTTGGAGGACATGATAGCTTGCATAAAAGATGAC4900.25388074858552157No Hit
CAACAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4700.24351826905141863No Hit
CAACAAGGGAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAA4660.24144577314459803No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4250.22020269009968707No Hit
CAACAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4050.20984021056558413No Hit
CAACAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3970.20569521875194297No Hit
CAACAAGGGAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGA3360.17408965617292904No Hit
CAACAAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3330.1725352842428136No Hit
CAACAAGGGAGTGTGGTATTTGTGTGTACCTATGTGTGGTATGTCTGACTATGTGT2840.14714720938426146No Hit
CAACAAGGGGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAGT2710.14041159768709457No Hit
CAACAAGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCGTCAGG2700.13989347371038943No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.13937534973368426No Hit
CAACAAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.1362666058734534No Hit
CAACAAGGGAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG2470.12797662224617107No Hit
CAACAAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG2440.12642225031605564No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2340.12124101054900417No Hit
CAACAAGGGATTAAGGAAATACCAGAATGACAGGACCATTGCAAGGGTATAATTTC2200.11398727487513212No Hit
CAACAAGGGGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGTCACCCAGA2170.11243290294501668No Hit
CAACAAGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.10828791113137552No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1950.10103417545750347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTTA204.0558242E-450.29456349
GTCCGCG150.00822709150.29456349
GACGTGC150.00824410850.26828848
CGGACTA150.00824410850.26828847
ACGAAAT204.066301E-450.26828447
CGCGGAC150.00826115350.2420445
TCCACGC301.0090989E-650.2420442
CGCCACA150.00826115350.2420442
TACCACC150.00830387650.17653340
TTGCTCG150.00832101150.1503836
CCTAATG150.00832101150.1503838
TCTACTG150.00832101150.1503838
AGCTGCG150.00832101150.1503836
TAGGCTA150.00832101150.1503837
TGGTATG355.1355528E-850.1503837
GGCCGAA150.00838981750.04603633
CGCGGTG150.00839844750.03302432
CTTCGCA150.00845904549.94212327
GCTGTTA150.008476417549.91621426
CGCGGCA204.2098537E-449.9162126
Produced by FastQC (version 0.11.5)