FastQCFastQC Report
Tue 31 May 2016
SRR212911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319174
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45111.4133356727051702No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28510.893243183968619No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA16650.521659032377324No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15960.5000407301346601No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15730.4928346293871054No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA9080.2844843251643304No Hit
TGCAATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8340.2612994792808938No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8060.25252683489256644No Hit
TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5990.1876719281645748No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA5870.18391222342672023No Hit
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT5380.1685600957471473No Hit
TGCAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5020.15728098153358355No Hit
TGCAATGGGAGAACTGTCACCCTTTTAGTCACCCCATAACCTCTGTAATTCAAGAGGC4900.15352127679572897No Hit
TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4380.13722922293169243No Hit
TGCAATGGGATCTAAGGAATTGGTTGCTGTCCTCACTCTTAACAATCCAGTTAGATTT4230.1325295920093742No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA4160.13033643091229236No Hit
TGCAATGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4150.1300231221841378No Hit
TGCAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3840.12031055161134678No Hit
TGCAATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3750.11749077305795584No Hit
TGCAATGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTATATATA3470.10871812866962847No Hit
TGCAATGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.10652496757254663No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA3230.1011987191939193No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3200.10025879300945566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTA150.00716439252.0933250
TTCGAAG150.00716439252.0933250
ACGACAG150.007204540552.01976835
ACGTACC150.00723139951.97084426
AGCGTAC150.007240368551.9545621
GCACGTC505.456968E-1251.9545621
ATGCGCC150.007240368551.9545621
CACGTCC505.456968E-1251.9545622
GCGTACG150.007240368551.9545622
GCAACGT203.4587824E-451.95455621
GTGGACG150.00724485651.946429
CTGCGGA150.00724485651.9464219
GATATCG308.033785E-751.946429
ATCGAGG150.00724485651.9464210
ATGGGAG42200.050.5923655
AATGGGA149000.050.5518844
CAATGGG328300.050.4986273
GCAATGG328400.050.483252
ATGGGAT46000.050.478375
TGCAATG328750.050.429511
Produced by FastQC (version 0.11.5)