##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212911_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 319174 Sequences flagged as poor quality 0 Sequence length 58 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.62450575548134 39.0 37.0 40.0 33.0 40.0 2 36.426328585661736 38.0 35.0 39.0 30.0 40.0 3 36.486023297637026 38.0 35.0 39.0 30.0 40.0 4 36.42287592347748 38.0 35.0 39.0 30.0 40.0 5 37.43316498211007 39.0 37.0 40.0 33.0 40.0 6 36.16536121363269 38.0 35.0 39.0 29.0 40.0 7 36.87128650829955 38.0 36.0 39.0 31.0 40.0 8 37.46652609548397 39.0 38.0 40.0 33.0 40.0 9 37.397591909115405 39.0 38.0 40.0 33.0 40.0 10 36.14337007400352 38.0 35.0 39.0 29.0 40.0 11 36.738333949507165 38.0 36.0 39.0 31.0 40.0 12 36.53594277729389 38.0 35.0 39.0 31.0 40.0 13 36.48054039489432 38.0 35.0 39.0 31.0 40.0 14 36.457872508412336 38.0 35.0 39.0 31.0 40.0 15 36.49062266976633 38.0 35.0 39.0 31.0 40.0 16 36.29246743155771 38.0 35.0 39.0 30.0 40.0 17 35.97925896219617 38.0 35.0 39.0 29.0 40.0 18 35.94527436445325 38.0 35.0 39.0 29.0 40.0 19 35.914306929762446 38.0 35.0 39.0 29.0 40.0 20 35.8187164368025 38.0 35.0 39.0 29.0 40.0 21 35.72869030685457 38.0 35.0 39.0 29.0 40.0 22 35.57141559149555 38.0 35.0 39.0 28.0 40.0 23 35.45167212868216 38.0 34.0 39.0 28.0 40.0 24 35.43590016730686 38.0 34.0 39.0 28.0 40.0 25 35.37933854261312 38.0 34.0 39.0 28.0 40.0 26 35.02531534523489 38.0 34.0 39.0 27.0 40.0 27 34.96870359114464 38.0 33.0 39.0 27.0 40.0 28 34.738468672260275 38.0 33.0 39.0 27.0 40.0 29 34.78246348386774 38.0 33.0 39.0 27.0 40.0 30 34.44063426218928 38.0 33.0 39.0 26.0 40.0 31 34.273885090890865 38.0 33.0 39.0 25.0 40.0 32 34.17064673187666 38.0 33.0 39.0 25.0 40.0 33 33.99993420516709 37.0 33.0 39.0 25.0 40.0 34 33.66280774749823 37.0 32.0 39.0 23.0 40.0 35 33.56966106261788 36.0 32.0 39.0 23.0 40.0 36 34.624317770244446 38.0 34.0 39.0 26.0 40.0 37 34.50987235802415 38.0 33.0 39.0 26.0 40.0 38 34.279183141483955 38.0 33.0 39.0 26.0 40.0 39 34.27071127347465 38.0 33.0 39.0 26.0 40.0 40 34.13519271619869 38.0 33.0 39.0 25.0 40.0 41 34.25204747253849 38.0 33.0 39.0 26.0 40.0 42 34.22764385570253 38.0 33.0 39.0 25.0 40.0 43 34.085843458427064 38.0 33.0 39.0 25.0 40.0 44 33.990910913796235 38.0 33.0 39.0 25.0 40.0 45 33.698684729959204 38.0 33.0 39.0 23.0 40.0 46 33.36661194207548 38.0 33.0 39.0 22.0 40.0 47 33.28477256919423 37.0 33.0 39.0 22.0 40.0 48 33.119320496030376 37.0 33.0 39.0 22.0 40.0 49 33.02162770150451 37.0 32.0 39.0 21.0 40.0 50 32.79255829108887 37.0 32.0 39.0 19.0 40.0 51 32.516141665674525 37.0 32.0 39.0 16.0 40.0 52 32.46133770294573 36.0 32.0 39.0 16.0 40.0 53 32.25353255590994 36.0 32.0 39.0 14.0 40.0 54 32.125173103072306 36.0 31.0 39.0 11.0 40.0 55 31.90245446057636 36.0 31.0 39.0 2.0 40.0 56 31.661263135468428 36.0 32.0 39.0 2.0 40.0 57 31.431141007726193 36.0 31.0 39.0 2.0 40.0 58 31.090223514446667 36.0 30.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -2.100201712581743 1 2 -2.6622053088103037 1 3 -2.8874955890901326 1 4 -6.827477382447821 1 5 -3.6342819080155095 1 6 -5.126718369275068 1 7 -3.882348322843413 1 8 -1.8738704112589701 1 9 -2.0193450572780307 1 10 -4.173711387574354 1 11 -3.196020219191645 1 12 -6.456051720221321 1 13 -5.535000883592154 1 14 -4.8609346641905375 1 15 -3.125036200058215 1 16 -5.218033702704023 1 17 -7.511494120378391 1 18 -3.6332836525634633 1 19 -6.095804575160415 1 20 -3.1732271747973932 1 21 -6.312413832065587 1 22 -2.0112128359059973 1 23 -6.074672772535642 1 24 -4.354745345487299 1 25 -3.9237862518017295 1 26 -0.2013828003968996 1 27 -4.668754159391387 1 28 -1.9471788005230373 1 29 -4.1972194553830455 1 30 -4.056443554221897 1 31 -11.781931592285165 1 32 -9.174996987097277 1 33 -5.513232970866323 1 34 -5.528849939556817 1 35 -7.586569869481451 1 36 -4.6732553149219385 1 37 -5.2249492773225725 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-4.3207366454298075 120 23 -1.0746727725356422 120 24 -2.2118882026301563 120 25 -3.4237862518017295 120 26 -3.1775732765873776 120 27 -1.5735160641532921 120 28 -3.0900359433801796 120 29 -3.221028979192571 120 30 -0.6278721256504696 120 31 -2.853360163713738 120 32 -4.841663653763945 120 33 -5.084661542294896 120 34 -0.00504041574729186 120 35 -4.277046059957641 120 36 -4.149445791112413 120 37 -2.6535207058939996 120 38 0.285438630117433 120 39 -1.9614015013302613 120 40 0.33494890821840784 120 41 1.4349986017723282 120 42 2.423800785944728 120 43 0.15943590081892012 120 44 1.8383924860036593 120 45 2.671804488093354 120 46 1.0645021164442454 120 47 2.6909705825652424 120 48 -2.583154133287227 120 49 -3.2940046880924143 120 50 -3.4821452001627904 120 51 -1.3198585977405521 120 52 -3.314984239443355 120 53 -1.77012900935976 120 54 -1.3232858891200614 120 55 -0.9309647290833603 120 56 -3.0317461367739824 120 57 -1.6258899986716635 120 58 -5.385375474778247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 150.0 3 15.0 4 65.0 5 76.0 6 126.0 7 185.0 8 306.0 9 416.0 10 524.0 11 550.0 12 645.0 13 790.0 14 847.0 15 978.0 16 1064.0 17 1147.0 18 1206.0 19 1374.0 20 1490.0 21 1660.0 22 1910.0 23 2353.0 24 2907.0 25 3545.0 26 4337.0 27 5536.0 28 6446.0 29 7774.0 30 9113.0 31 10635.0 32 12758.0 33 15736.0 34 19245.0 35 23779.0 36 29330.0 37 42536.0 38 52852.0 39 54333.0 40 435.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 43.68463596658876 44.44597617600431 3.5585605343793665 8.310827323027564 11 31.39541441345473 24.934048512723468 26.43479732058376 17.235739753238047 12 31.86788397551179 18.662861009982016 25.389912712188334 24.07934230231786 13 22.790390194690044 20.737904716549593 30.17351037365199 26.298194715108377 14 21.72326066659565 25.446621591984307 31.46872865584289 21.361389085577144 15 26.154072700157283 27.897949081065498 26.786643022301316 19.161335196475903 16 30.647546479349824 30.353662892340854 22.258705283011775 16.74008534529755 17 26.313942595394973 29.602441309258616 24.443483628001115 19.640132467345296 18 25.579777801449993 29.64464524052711 25.374560584508764 19.401016373514132 19 23.766002644391946 32.85938978462618 23.91388807078448 19.460719500197392 20 26.828794505783716 30.81911947163285 24.212963543167948 18.139122479415487 21 25.21094237113951 30.066768391072902 25.49668977920373 19.225599458583858 22 22.858136496508163 27.98106356114082 28.3667478138815 20.794052128469513 23 22.934989331470128 30.079927811181122 27.48783536938806 19.4972474879607 24 24.74614080764977 30.184570750032115 25.450461035237943 19.618827407080172 25 22.373759685186403 32.15873523136356 22.92142505788397 22.546080025566074 26 20.84360947023707 32.4078281443412 26.43455915764403 20.31400322777769 27 19.373981701967665 29.06191252036596 26.009525003133227 25.554580774533147 28 20.33827128768102 32.7963947814587 25.01527767766764 21.850056253192644 29 24.820999395236466 29.249873877035593 24.755196109459064 21.173930618268873 30 21.705766554236206 28.15129211440322 28.421187917695885 21.72175341366469 31 25.06614171253746 28.807380286634988 25.95168833774278 20.174789663084773 32 26.028642161010314 29.088402118391777 26.31945097301871 18.563504747579206 33 25.465484285400937 28.919875912637483 27.085200388556384 18.529439413405196 34 21.06269292843824 29.81212491577743 30.975101458813203 18.15008069697112 35 19.51328057666693 34.45490872052026 28.23756170179425 17.79424900101857 36 19.86012144543263 35.870291011345145 26.00386844769915 18.26571909552307 37 24.612895843195776 32.32833078869239 25.64496458925733 17.413808778854502 38 21.127299411074805 35.71495769648087 25.256988390835595 17.900754501608727 39 24.321011264488178 31.377540623324666 25.92932811231256 18.372119999874595 40 20.908803029970496 31.497082587389123 25.355623348079774 22.2384910345606 41 18.916766478330942 33.9314717151511 26.523669473935048 20.628092332582916 42 19.44859121358858 31.577577338986877 25.90260180144015 23.071229645984396 43 19.326500272862422 30.966434785881408 25.91158003023441 23.79548491102176 44 20.939005117399155 30.43253311258278 26.135422937989162 22.493038832028898 45 22.887682761258844 31.263894245391576 25.65884983083903 20.189573162510545 46 20.473687841405823 33.99194736941129 26.10582561413851 19.42853917504437 47 19.543878911457924 30.99364755705435 27.177162575484274 22.28531095600345 48 20.714814489516804 30.162549090954716 28.963035922659003 20.15960049686947 49 22.434220021712257 30.430731000207086 26.770082268114987 20.364966709965675 50 19.426018086768796 30.03740358848522 27.95510314227798 22.581475182468008 51 18.689630291907832 30.41111790817687 30.061016736467305 20.838235063447993 52 19.093609533159977 30.446702195399666 28.55336994946723 21.90631832197313 53 18.684753038190358 30.77475815283047 30.74526268226789 19.795226126711288 54 19.45008425723404 30.288043631753524 29.773713625822566 20.48815848518987 55 19.410719666532746 30.849173865006023 26.483088087585376 23.257018380875856 56 19.22848720580895 32.485096203652255 25.70139667172093 22.585019918817867 57 22.04925366106324 33.37225910753246 24.612390325061607 19.966096906342703 58 20.364184352630918 36.459876930804974 24.718071078739168 18.45786763782494 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 931.5 9 4431.0 10 6999.0 11 7926.5 12 8854.0 13 6523.0 14 4192.0 15 3420.5 16 2499.0 17 2349.0 18 2199.0 19 2049.0 20 2074.0 21 2099.0 22 2500.0 23 3381.5 24 3862.0 25 4282.5 26 4703.0 27 4954.0 28 5714.5 29 6224.0 30 6330.5 31 6437.0 32 7056.5 33 7676.0 34 7713.0 35 8259.5 36 8769.0 37 8980.0 38 9191.0 39 9615.0 40 10039.0 41 9961.0 42 10820.5 43 11758.0 44 11919.0 45 12080.0 46 13154.0 47 13990.0 48 13752.0 49 14266.5 50 14781.0 51 15578.0 52 16375.0 53 17216.0 54 17872.5 55 17688.0 56 18206.0 57 18724.0 58 19357.0 59 18394.0 60 16798.0 61 14482.0 62 12166.0 63 9636.5 64 7107.0 65 5758.5 66 3684.5 67 2959.0 68 2290.0 69 1621.0 70 1391.0 71 1161.0 72 995.5 73 620.5 74 411.0 75 315.0 76 219.0 77 181.5 78 126.0 79 108.0 80 80.0 81 52.0 82 38.5 83 25.0 84 23.5 85 14.5 86 7.0 87 6.5 88 6.0 89 3.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 3.133087281545489E-4 18 0.0 19 0.0025064698252363913 20 6.266174563090978E-4 21 0.003446396009700038 22 3.133087281545489E-4 23 0.003446396009700038 24 3.133087281545489E-4 25 3.133087281545489E-4 26 0.021618302242663874 27 0.004386322194163685 28 0.025378006980518463 29 0.012845657854336506 30 0.050756013961036926 31 0.050756013961036926 32 0.02005175860189113 33 0.013785584038800152 34 0.024751389524209366 35 0.03101756408730034 36 0.05733549725228245 37 0.06360167181537343 38 0.09023291370851008 39 0.06485490672799163 40 0.0711210812910826 41 0.07519409475709174 42 0.09963217555314656 43 0.10401849774731024 44 0.08208688677649181 45 0.07738725585417358 46 0.0852199740580373 47 0.12501018253366503 48 0.11843069924241949 49 0.1441220149510925 50 0.15289465933941987 51 0.12814326981521051 52 0.11623753814533765 53 0.15070149824233803 54 0.1588475251743563 55 0.18234567978594748 56 0.19769780746552038 57 0.1939381027276658 58 0.204903908213075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 319174.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.785402319737827 #Duplication Level Percentage of deduplicated Percentage of total 1 58.39973946406944 17.97859474769248 2 13.16927711456457 8.108429884639726 3 7.701075728432001 7.1124214378364154 4 5.056025402253229 6.226071045887196 5 3.5233413733093153 5.423374084355242 6 2.5677037217964767 4.742867526803562 7 1.9519840421742536 4.2064829842029745 8 1.4431248028170447 3.554174212185203 9 1.1337383852878615 3.1412333084775077 >10 4.71305427492647 23.61313891482389 >50 0.2198271914023143 4.71654959363858 >100 0.10889587722244273 5.859813142674529 >500 0.007124029351000926 1.4957358682098167 >1k 0.00508859239357209 3.8211132485728787 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4511 1.4133356727051702 No Hit TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2851 0.893243183968619 No Hit TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 1665 0.521659032377324 No Hit TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1596 0.5000407301346601 No Hit TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 1573 0.4928346293871054 No Hit TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 908 0.2844843251643304 No Hit TGCAATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 834 0.2612994792808938 No Hit TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 806 0.25252683489256644 No Hit TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 599 0.1876719281645748 No Hit TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 587 0.18391222342672023 No Hit TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 538 0.1685600957471473 No Hit TGCAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 502 0.15728098153358355 No Hit TGCAATGGGAGAACTGTCACCCTTTTAGTCACCCCATAACCTCTGTAATTCAAGAGGC 490 0.15352127679572897 No Hit TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 438 0.13722922293169243 No Hit TGCAATGGGATCTAAGGAATTGGTTGCTGTCCTCACTCTTAACAATCCAGTTAGATTT 423 0.1325295920093742 No Hit TGCAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA 416 0.13033643091229236 No Hit TGCAATGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 415 0.1300231221841378 No Hit TGCAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 384 0.12031055161134678 No Hit TGCAATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 375 0.11749077305795584 No Hit TGCAATGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTATATATA 347 0.10871812866962847 No Hit TGCAATGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 340 0.10652496757254663 No Hit TGCAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA 323 0.1011987191939193 No Hit TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 320 0.10025879300945566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0012532349126181956 0.0 0.0 0.0 0.0 11 0.005639557106781881 0.0 0.0 0.0 0.0 12 0.00595286583493643 0.0 0.0 0.0 0.0 13 0.006266174563090979 0.0 0.0 0.0 0.0 14 0.008146026932018272 0.0 0.0 0.0 0.0 15 0.009085953116481919 0.0 0.0 0.0 0.0 16 0.009712570572791016 0.0 0.0 0.0 0.0 17 0.010025879300945565 0.0 0.0 0.0 0.0 18 0.010965805485409213 0.0 0.0 0.0 0.0 19 0.012532349126181957 0.0 0.0 0.0 0.0 20 0.015352127679572898 0.0 0.0 0.0 0.0 21 0.016292053864036544 0.0 0.0 0.0 0.0 22 0.01785859750480929 0.0 0.0 0.0 0.0 23 0.019425141145582032 0.0 0.0 0.0 0.0 24 0.020678376058200228 0.0 0.0 0.0 0.0 25 0.02130499351450933 0.0 0.0 0.0 0.0 26 0.02381146333974572 0.0 0.0 0.0 0.0 27 0.02600462443682756 0.0 0.0 0.0 0.0 28 0.03415065136884583 0.0 0.0 0.0 0.0 29 0.03540388628146403 0.0 0.0 0.0 0.0 30 0.039163591019318614 0.0 0.0 0.0 0.0 31 0.03979020847562771 0.0 0.0 0.0 0.0 32 0.041356752116400455 0.0 0.0 0.0 0.0 33 0.042923295757173206 0.0 0.0 0.0 0.0 34 0.043549913213482304 0.0 0.0 0.0 0.0 35 0.04950277904841873 0.0 0.0 0.0 0.0 36 0.05012939650472783 0.0 0.0 0.0 0.0 37 0.05044270523288238 0.0 0.0 0.0 0.0 38 0.051069322689191475 0.0 0.0 0.0 0.0 39 0.05294917505811877 0.0 0.0 0.0 0.0 40 0.05294917505811877 0.0 0.0 0.0 0.0 41 0.05545564488335516 0.0 0.0 0.0 0.0 42 0.057648805980437 0.0 0.0 0.0 0.0 43 0.05984196707751885 0.0 0.0 0.0 0.0 44 0.061095201990137044 0.0 0.0 0.0 0.0 45 0.06203512817460069 0.0 0.0 0.0 0.0 46 0.06328836308721889 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGTTA 15 0.007164392 52.09332 50 TTCGAAG 15 0.007164392 52.09332 50 ACGACAG 15 0.0072045405 52.019768 35 ACGTACC 15 0.007231399 51.970844 26 AGCGTAC 15 0.0072403685 51.95456 21 GCACGTC 50 5.456968E-12 51.95456 21 ATGCGCC 15 0.0072403685 51.95456 21 CACGTCC 50 5.456968E-12 51.95456 22 GCGTACG 15 0.0072403685 51.95456 22 GCAACGT 20 3.4587824E-4 51.954556 21 GTGGACG 15 0.007244856 51.94642 9 CTGCGGA 15 0.007244856 51.94642 19 GATATCG 30 8.033785E-7 51.94642 9 ATCGAGG 15 0.007244856 51.94642 10 ATGGGAG 4220 0.0 50.592365 5 AATGGGA 14900 0.0 50.551884 4 CAATGGG 32830 0.0 50.498627 3 GCAATGG 32840 0.0 50.48325 2 ATGGGAT 4600 0.0 50.47837 5 TGCAATG 32875 0.0 50.42951 1 >>END_MODULE