FastQCFastQC Report
Tue 31 May 2016
SRR212910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342195
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47931.400663364455939No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30520.8918891275442365No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA17700.5172489370095998No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA17030.4976694574730782No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16780.4903636815266149No Hit
TGCAATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8970.2621312409591023No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA8550.24985753736904395No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8070.23583044755183447No Hit
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6440.18819678838089393No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA5910.1727085433743918No Hit
TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5880.1718318502608162No Hit
TGCAATGGGAGAACTGTCACCCTTTTAGTCACCCCATAACCTCTGTAATTCAAGAGGC5480.16014260874647496No Hit
TGCAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4960.14494659477783137No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA4810.1405631292099534No Hit
TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4560.1332573532634901No Hit
TGCAATGGGATCTAAGGAATTGGTTGCTGTCCTCACTCTTAACAATCCAGTTAGATTT4310.12595157731702683No Hit
TGCAATGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4150.12127588071129033No Hit
TGCAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3800.11104779438624177No Hit
TGCAATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.1104633323105247No Hit
TGCAATGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTATATATA3510.10257309428834437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAT600.052.0710450
TACATCG150.007181122452.06341649
CGAGACG150.007189444752.04817241
CGTACTG203.4285264E-452.0481742
ACGTACT203.4285264E-452.0481741
TTACGGT150.00720194252.0253237
TTACCCG150.00720194252.0253237
CCCGTCG150.00720611152.01770835
TCCACGA150.00721028252.01009432
CGAGTTA203.4533834E-451.97207622
CTCACCG150.007235346451.96447812
CCGTCTA150.007235346451.96447815
CCCGGTA150.007235346451.96447813
TCGTTAG308.0243444E-751.95688620
CGGTGAC150.007239530351.95688620
GATACTA150.007243715751.9492959
ATGGGAT48350.050.821135
AATGGGA156500.050.604924
CAATGGG351500.050.567423
GCAATGG351750.050.5314872
Produced by FastQC (version 0.11.5)