FastQCFastQC Report
Tue 31 May 2016
SRR212909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359947
Sequences flagged as poor quality0
Sequence length58
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46091.2804662908706004No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29240.8123418169897235No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA17150.4764590342467085No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA16250.4514553531492136No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15820.4395091499581883No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA8310.23086732213353633No Hit
TGCAATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8210.22808913534492578No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7320.20336327292629192No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA5800.16113483373941165No Hit
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT5730.15919010298738426No Hit
TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5560.1544671854467463No Hit
TGCAATGGGAGAACTGTCACCCTTTTAGTCACCCCATAACCTCTGTAATTCAAGAGGC5220.14502135036547045No Hit
TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4630.12863004831266825No Hit
TGCAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.12585186152405772No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA4050.1125165649387271No Hit
TGCAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4000.11112747154442182No Hit
TGCAATGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.10890492211353336No Hit
TGCAATGGGATCTAAGGAATTGGTTGCTGTCCTCACTCTTAACAATCCAGTTAGATTT3820.10612673532492284No Hit
TGCAATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3710.10307072985745123No Hit
TGCAATGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTATATATA3630.1008481804265628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGC150.007203802552.0224543
AACCGGT203.439501E-452.01519825
TAGCGGT150.007207775552.01519824
ATAGCGG150.007207775552.01519826
ACTAACG150.007207775552.01519826
CGACATT150.007211750452.0079542
TTGACGA150.00721572752.00069852
AATAACG150.00721970551.99345451
TAGGTAG150.007227665751.97896650
ACCTAGT150.00723563351.96448534
CGTGCGC150.007239619751.95725323
CGGGAAT150.007239619751.95725330
CTTACCG251.6623362E-551.95001648
AGTTGCG150.00724759751.94278346
CGAGTTA150.007287581551.8705722
GCGAGAC150.00729158951.8633614
TACATCG150.007295598351.8561513
CATACGC150.007295598351.8561520
ATACGCC150.007295598351.8561521
CGGCATA203.491901E-451.85614820
Produced by FastQC (version 0.11.5)