FastQCFastQC Report
Tue 31 May 2016
SRR212908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212908_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562834
Sequences flagged as poor quality0
Sequence length56
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71981.278885070909007No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48940.869528137958972No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC40510.7197504059811596No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT25800.4583944822096746No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21840.38803625935888736No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC21050.37400014924471514No Hit
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT17970.3192770870274361No Hit
TGCAATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15790.2805445300035179No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC10790.19170839004040266No Hit
TGCAATGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTATATA9570.17003237188940257No Hit
TGCAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8610.15297583301648443No Hit
TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC8290.14729032005884507No Hit
TGCAATGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8100.14391454674024667No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCA7150.12703568014725478No Hit
TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7050.12525895734799247No Hit
TGCAATGGGAGAACTGTCACCCTTTTAGTCACCCCATAACCTCTGTAATTCAAGAG6930.12312688998887772No Hit
TGCAATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6900.12259387314909902No Hit
TGCAATGGGATTGCACTAGACTTTTATGCTGCACCCAACACCCTCTTTTCCCCAGC6530.11601999879182849No Hit
TGCAATGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6090.10820241847507436No Hit
TGCAATGGGATCTAAGGAATTGGTTGCTGTCCTCACTCTTAACAATCCAGTTAGAT5850.10393828375684483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG150.008271323550.23907550
CCGGGTT150.00828890350.21209745
ACGTAAC150.00831532550.17168841
CACCGTT204.1155348E-450.1627239
ATACCGA150.00832120450.16271639
TTCGTTG150.00833297350.14478336
CCTCGCG150.00833297350.14478336
CGCTATA150.00839792550.0463831
GCGCTAA150.00840977550.0285330
AACGCGA150.008475170549.93058423
CGTACCA150.00847815249.9261422
CTCGCAC150.00849008649.9083821
CGAACTC150.00853195249.84630212
CGTTAGT150.00853195249.84630217
GTTGCGA150.00853195249.8463029
ATGGGAT89850.048.1819845
AATGGGA264450.047.763494
AATGGGC50100.047.756944
GCAATGG589300.047.6047862
CAATGGG589450.047.5926673
Produced by FastQC (version 0.11.5)