FastQCFastQC Report
Tue 31 May 2016
SRR212907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502642
Sequences flagged as poor quality0
Sequence length56
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62581.245021307411637No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42860.8526943629859821No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC36700.7301419300416598No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT23090.45937267478642857No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC19580.3895416618587384No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19220.38237950668666765No Hit
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT15400.3063810823608055No Hit
TGCAATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14300.2844967193350337No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC10290.2047182686683564No Hit
TGCAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8320.1655253639767469No Hit
TGCAATGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTATATA7620.1515989511421648No Hit
TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC7600.1512010536326053No Hit
TGCAATGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6960.13846833332670172No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCA6950.13826938457192198No Hit
TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6800.1352851532502258No Hit
TGCAATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6370.12673035679469682No Hit
TGCAATGGGATTGCACTAGACTTTTATGCTGCACCCAACACCCTCTTTTCCCCAGC6270.12474086924689938No Hit
TGCAATGGGAGAACTGTCACCCTTTTAGTCACCCCATAACCTCTGTAATTCAAGAG5550.11041655890275782No Hit
TGCAATGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5070.10086701867333013No Hit
TGCAATGGGATCTAAGGAATTGGTTGCTGTCCTCACTCTTAACAATCCAGTTAGAT5040.10027017240899089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA355.0988092E-850.2238848
CGAATCC204.096511E-450.20878245
CGTACTG252.0373998E-550.19871542
CGCCCGA301.0219628E-650.1735840
TACGTAC252.0434469E-550.17357640
CTACGTA252.0458698E-550.1635339
TCTACGT252.0470821E-550.15850438
CTCCGAT150.00833003750.14846435
CATGCGG150.00840942950.02826329
TCGATCC150.00840942950.02826329
TATTCCG301.0426138E-650.02826328
AACCGGT150.00844600549.97336625
ATCTCGA150.0085396449.83416410
CCTACCG150.0085396449.83416416
GAACGTA150.0085396449.8341649
ATGGGAT75000.048.438815
AATGGGA230800.047.901684
CAATGGG526100.047.6081543
GCAATGG526500.047.5719832
TGCAATG526600.047.5629541
Produced by FastQC (version 0.11.5)