FastQCFastQC Report
Tue 31 May 2016
SRR212906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144708
Sequences flagged as poor quality0
Sequence length58
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29522.03997014677834No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22741.5714404179451031No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17431.2044945683721702No Hit
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7880.5445448765790419No Hit
CGCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7290.5037731155153827No Hit
CGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6230.4305221549603339No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5380.37178317715675707No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA4100.28332918705254717No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA4000.2764187190756558No Hit
CGCTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3970.27434557868258835No Hit
CGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.2308096304281726No Hit
CGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3290.22735439643972688No Hit
CGCTTGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.20869613290212014No Hit
CGCTTGGGGTATTATCTTGGTTATTGATAAAGCAAAGCACCTTTATGTGTATTCTTTA2890.19971252453216132No Hit
CGCTTGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.18658263537606767No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2590.17898112060148713No Hit
CGCTTGGGGAGGAAGACTGATGTTGGAGACAATCGGGTGCGGATTGGTGCTAATTTAA2490.17207065262459575No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2180.1506482018962324No Hit
CGCTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA2070.1430466871216519No Hit
CGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.14235564032396272No Hit
CGCTTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2050.14166459352627359No Hit
CGCTTGGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT2040.14097354672858448No Hit
CGCTTGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.14097354672858448No Hit
CGCTTGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.13890040633551703No Hit
CGCTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1990.13751831274013876No Hit
CGCTTGGGGGCTTTCTTTTGCCATCTAACCTGCAGCACTGTCAGGACATGGCCTGTGG1970.13613621914476048No Hit
CGCTTGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.12646156397711253No Hit
CGCTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1700.11747795560715373No Hit
CGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1660.11471376841639717No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1650.11402272161870801No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.11264062802332975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATA203.4177513E-452.0609450
AGTATAG203.4177513E-452.0609451
AGCGATG150.00717412552.0609450
GCGATGG150.00717412552.0609451
TGATACT203.423601E-452.0429238
CTCACGG150.007183957452.0429233
CATATTC150.007183957452.0429248
CGACGGG150.007183957452.0429244
CCCTATA150.007183957452.0429231
ACACCCG150.007183957452.0429233
TAAGTCC150.007183957452.0429234
GTAGGAT307.9010897E-752.0429233
GCGTTGC150.007183957452.0429235
ACGGGCG150.007183957452.0429246
CCGTCTC150.007183957452.0429248
TCGCTGA150.007183957452.0429243
GAAACTA150.007183957452.0429235
GTCCGCC150.007183957452.0429247
CCGACGG203.423601E-452.0429243
CCGACCG150.007183957452.0429243
Produced by FastQC (version 0.11.5)