FastQCFastQC Report
Tue 31 May 2016
SRR212905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148174
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30012.0253215813840484No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22051.4881153238759837No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16461.1108561556008476No Hit
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8270.5581276067326252No Hit
CGCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7350.49603844129199454No Hit
CGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6060.4089786332285016No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5970.40290469313104865No Hit
CGCTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4530.3057216515718007No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA4120.2780514800167371No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA3920.2645538353557304No Hit
CGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3460.2335092526354151No Hit
CGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3280.22136137244050913No Hit
CGCTTGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780.18761726078799248No Hit
CGCTTGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.18694237855494217No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2740.18491773185579116No Hit
CGCTTGGGGTATTATCTTGGTTATTGATAAAGCAAAGCACCTTTATGTGTATTCTTTA2600.1754693805930865No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2480.1673707937964825No Hit
CGCTTGGGGAGGAAGACTGATGTTGGAGACAATCGGGTGCGGATTGGTGCTAATTTAA2420.16332150039818052No Hit
CGCTTGGGGGCTTTCTTTTGCCATCTAACCTGCAGCACTGTCAGGACATGGCCTGTGG2200.1484740912710732No Hit
CGCTTGGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT2080.14037550447446923No Hit
CGCTTGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.13970062224141888No Hit
CGCTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA1990.1343015643770162No Hit
CGCTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1950.13160203544481489No Hit
CGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1940.13092715321176454No Hit
CGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1830.12350344864821088No Hit
CGCTTGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.12350344864821088No Hit
CGCTTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1780.12012903748295924No Hit
CGCTTGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.1174295085507579No Hit
CGCTTGGGGATCTTCTTCTCTTCTATCATCCAAATCAAATGTAAGAAGTCCCTCACAT1710.11540486185160688No Hit
CGCTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1570.10595651058890224No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1520.10258209942365058No Hit
CGCTTGGGGAGGTTAGGCTGCCCATTTCTCCCGAGCTCATGGGAAGGCTGTGCATAGG1490.10055745272449956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCT203.4112413E-452.0818724
TAACAGC203.4112413E-452.0818726
CGAGACT203.4112413E-452.0818727
CCACCCG203.4112413E-452.0818724
ACGCGAT150.007163066452.08186724
CACCCGT150.007163066452.08186725
ATCTAAC203.4226567E-452.04665423
CGTTGGC150.007182258652.04665452
CCGTGGG150.007182258652.04665421
CAATTAC150.007182258652.04665422
CCATCTA203.4226567E-452.04665421
ACGGATT150.007182258652.04665421
CGATGGA150.007182258652.04665452
AAGTATA203.428376E-452.02906450
AACGTAG203.428376E-452.02906449
TATGGGT150.00719186952.02906449
GACATCC203.4341027E-452.0114938
ATTATAG203.4341027E-452.0114937
AAGTAAC150.007201488652.01148635
AATTTGG150.007201488652.01148642
Produced by FastQC (version 0.11.5)