FastQCFastQC Report
Tue 31 May 2016
SRR212904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152517
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27871.8273372804343122No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20091.3172302104027749No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14940.9795629339680166No Hit
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7510.49240412544175405No Hit
CGCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7280.47732383930971634No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5680.3724175009998886No Hit
CGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5510.3612712025544693No Hit
CGCTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4630.3035727164840641No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA3920.257020528859078No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3800.24915255348584095No Hit
CGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.19932204278867274No Hit
CGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.18555308588550784No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2760.18096343358445288No Hit
CGCTTGGGGTATTATCTTGGTTATTGATAAAGCAAAGCACCTTTATGTGTATTCTTTA2690.17637378128339792No Hit
CGCTTGGGGAGGAAGACTGATGTTGGAGACAATCGGGTGCGGATTGGTGCTAATTTAA2660.17440678744008864No Hit
CGCTTGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2650.17375112282565222No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2550.167194476681288No Hit
CGCTTGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.1508028613203774No Hit
CGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.14162355671826748No Hit
CGCTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2070.13572257518833966No Hit
CGCTTGGGGGCTTTCTTTTGCCATCTAACCTGCAGCACTGTCAGGACATGGCCTGTGG1910.12523194135735688No Hit
CGCTTGGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT1890.12392061212848403No Hit
CGCTTGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.11867529521299265No Hit
CGCTTGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.11801963059855623No Hit
CGCTTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1770.11605263675524696No Hit
CGCTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA1750.11474130752637411No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1540.10097235062320922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAT203.3141708E-452.388089
GGGTACG203.3141708E-452.388087
GGAGTCC150.00699922452.388088
GGTTCGG150.00699922452.388089
GTACGTA203.4240092E-452.0434239
ACGCGAT150.00719375652.02630624
AGACTTA203.429576E-452.02630629
CATATTA150.00719375652.02630626
CTCCGAC150.00719375652.02630629
CCCTATT150.00719375652.02630628
CTAAGCG150.00719375652.02630638
CGGGGCT150.00719375652.02630628
ACCTATT203.429576E-452.02630629
TCTACGT150.00719375652.02630638
AACGCTA150.00719375652.02630626
ACGCTAG150.00719375652.02630627
CGAGACT203.429576E-452.02630627
GTCCCGT203.429576E-452.02630628
CTCCTAG150.00719375652.02630629
GCTCGTT150.00719375652.02630638
Produced by FastQC (version 0.11.5)