FastQCFastQC Report
Tue 31 May 2016
SRR212903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179713
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31751.7667058031416758No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23421.3031889735300173No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18111.0077178612565592No Hit
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT17810.9910245780772677No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9600.5341850617373256No Hit
CGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7220.4017516818482803No Hit
CGCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7050.39229215471334855No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT4420.24594770550822703No Hit
CGCTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3990.22202066628457598No Hit
CGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.20421449755999846No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC3550.19753718428828188No Hit
CGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.19586785597035272No Hit
CGCTTGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3370.187521214380707No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3310.18418255774484873No Hit
CGCTTGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.1697150456561295No Hit
CGCTTGGGGTATTATCTTGGTTATTGATAAAGCAAAGCACCTTTATGTGTATTCTT2930.1630377323844129No Hit
CGCTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2900.16136840406648378No Hit
CGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2640.14690089197776454No Hit
CGCTTGGGGAGGCTTGAAGGCGTGCACATGCATCCATTGACCCATCTTACCAGCCC2610.14523156365983542No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.12853828048054397No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACTGACTGCTGTTCC2290.1274253949352579No Hit
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCTTGGGGGAGCAGG2260.12575606661732874No Hit
CGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2190.1218609672088274No Hit
CGCTTGGGGGCATGAGTTGCTGCTCGGTTGTCATAGGCCCAGCCACTGAGCCTGCC2170.12074808166354131No Hit
CGCTTGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.11907875334561217No Hit
CGCTTGGGGATCTTCTTCTCTTCTATCATCCAAATCAAATGTAAGAAGTCCCTCAC2110.11740942502768303No Hit
CGCTTGGGGAGGAAGACTGATGTTGGAGACAATCGGGTGCGGATTGGTGCTAATTT2090.11629653948239693No Hit
CGCTTGGGGATCTGACAGCTGCATTGTGTTCCTTGCCCTATTCTCAAATGCTCATG2040.1135143256191817No Hit
CGCTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2030.11295788284653865No Hit
CGCTTGGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGG2030.11295788284653865No Hit
CGCTTGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT2000.1112885545286095No Hit
CGCTTGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.10961922621068036No Hit
CGCTTGGGGGCTTTCTTTTGCCATCTAACCTGCAGCACTGTCAGGACATGGCCTGT1920.10683701234746512No Hit
CGCTTGGGGACTGCTTGCTGACTTCATCTCTGTGGCTTACTCAACTGGTTTTCTTA1910.10628056957482207No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1860.10349835571160683No Hit
CGCTTGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.10349835571160683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC150.00825901650.24390448
GTACGGG150.00825901650.24390449
TAATCCA150.00825901650.24390449
CCGTGTA150.00826817550.22982445
AGTCTAT150.00826817550.22982446
GTCCGCC150.00826817550.22982447
AATCTGT204.0809365E-450.22981647
GTCCTAA150.00827734150.21574842
CGTTAAT252.0280155E-550.21574843
GTTAATC252.0280155E-550.21574844
CTAAACC150.00827734150.21574842
GCGTGGG150.00827734150.21574842
TTGGCCG150.00828651550.20168341
TAGCAAA150.00830488650.17357340
TAAGACT150.00830488650.17357340
CGGATCT150.00832328750.14549638
GATACTC150.008378671550.06145534
CTACTAA150.008378671550.06145534
GTCATAG402.6448106E-950.033530
GCGACTA150.00839719450.033531
Produced by FastQC (version 0.11.5)