FastQCFastQC Report
Tue 31 May 2016
SRR212902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169280
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29291.7302693761814745No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21501.2700850661625709No Hit
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT16750.9894848771266541No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15390.9091446124763706No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9400.5552930056710775No Hit
CGCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7280.43005671077504726No Hit
CGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6750.39874763705103966No Hit
CGCTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4570.2699669187145558No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3760.222117202268431No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3460.20439508506616258No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC3280.1937618147448015No Hit
CGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.18431001890359167No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACTGACTGCTGTTCC2840.16776937618147447No Hit
CGCTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2790.1648156899810964No Hit
CGCTTGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.16363421550094517No Hit
CGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.15772684310018903No Hit
CGCTTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC2620.15477315689981097No Hit
CGCTTGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610.15418241965973534No Hit
CGCTTGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT2600.15359168241965973No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.14827504725897922No Hit
CGCTTGGGGGCATGAGTTGCTGCTCGGTTGTCATAGGCCCAGCCACTGAGCCTGCC2500.1476843100189036No Hit
CGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.14709357277882798No Hit
CGCTTGGGGAGGCTTGAAGGCGTGCACATGCATCCATTGACCCATCTTACCAGCCC2400.14177693761814747No Hit
CGCTTGGGGTATTATCTTGGTTATTGATAAAGCAAAGCACCTTTATGTGTATTCTT2350.13882325141776938No Hit
CGCTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2200.12996219281663515No Hit
CGCTTGGGGATCTGACAGCTGCATTGTGTTCCTTGCCCTATTCTCAAATGCTCATG2080.12287334593572778No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2080.12287334593572778No Hit
CGCTTGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.11873818525519848No Hit
CGCTTGGGGATCTTCTTCTCTTCTATCATCCAAATCAAATGTAAGAAGTCCCTCAC1990.11755671077504726No Hit
CGCTTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1960.11578449905482041No Hit
CGCTTGGGGAGGAAGACTGATGTTGGAGACAATCGGGTGCGGATTGGTGCTAATTT1940.11460302457466919No Hit
CGCTTGGGGGCTTTCTTTTGCCATCTAACCTGCAGCACTGTCAGGACATGGCCTGT1940.11460302457466919No Hit
CGCTTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC1870.11046786389413989No Hit
CGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1860.10987712665406428No Hit
CGCTTGGGGGACCCCAGCAGACAGTCCCAGAGACTGGACTCCTTACCACTGGCCCT1750.1033790170132325No Hit
CGCTTGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.10278827977315691No Hit
CGCTTGGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGG1720.10160680529300568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATA150.00822397750.2966650
AACGTAG150.00822397750.2966649
AACTTAG150.00822397750.2966649
CGTAGTG150.00823367750.2816747
GCGTAGT150.00824338650.2666946
TGCGTAG150.00826282850.23674845
AATATCC150.00827256150.22179443
CAACTGG355.0731614E-850.22179442
CGTTAGC150.00828230450.2068541
GTAATAA150.00829205550.19190640
CCGTTAG150.00829205550.19190640
TCCGTTA150.00830181450.1769839
ATTGGTT150.00830181450.1769839
CGTTGTA204.1015033E-450.17697539
CCCTATT451.3096724E-1050.1620636
GTCAGTA204.1075292E-450.1620636
TGTTCCG150.00831158350.1620636
ACAGATC204.1075292E-450.1620636
ACGCTAC150.00831158350.1620638
GACGCTA150.00831158350.1620637
Produced by FastQC (version 0.11.5)