FastQCFastQC Report
Tue 31 May 2016
SRR212901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64574
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11311.751478923405705No Hit
ACACCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9941.539319230650107No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4060.6287360237866634No Hit
ACACCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4010.620992969306532No Hit
ACACCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3170.4909096540403258No Hit
ACACCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610.404187443862855No Hit
ACACCCGGGATTTAAAGTTTGTTCTCTTAAGAAATTCAACAGTTTGCTTCCTCTTCCC2390.3701180041502772No Hit
ACACCCGGGGGGGATATGCTTAGTAATGCTGTGTGATTTTCTGGAGGTTGATAACTTT2070.3205624554774367No Hit
ACACCCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.2942360702449902No Hit
ACACCCGGGAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCACT1710.26481246322049123No Hit
ACACCCGGGATCACTATGGTATCAAAGACTTGGCTACAGTTTTGTTCTACATGCTAGT1580.24468052157214978No Hit
ACACCCGGGGTCAAGTTCAGCATCTTTGTTTCATTCCTGTTGATATGTGTGACTTTCC1540.2384860779880447No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1440.22299996902778207No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1440.22299996902778207No Hit
ACACCCGGGGCCCTGGCCCATCATAGTTTGTACTTCATGAAATATATTGTACATTTTA1420.21990274723572953No Hit
ACACCCGGGGGACCAATGCCCCAGTTGTCAGTCAAAGCCGTTGGTGTTTTCATTGTTT1340.20751386006751943No Hit
ACACCCGGGAATTGTAAAATACTATGTACCTTCAATGAAATGCAAGTTACTAAGCGTG1340.20751386006751943No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1280.19822219469136185No Hit
ACACCCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.18583330752315175No Hit
ACACCCGGGGTTTGGATGTATAAATAACTCAACTTCCCCAAATGTCAAGCTCTTTGTG1200.18583330752315175No Hit
ACACCCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGC1130.17499303125096788No Hit
ACACCCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.16879858766686281No Hit
ACACCCGGGATGGTTCAAGGTAAAACACTTTAAACAAGTTAAAAAATCAAAATGCTTC1090.16879858766686281No Hit
ACACCCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.1610555331867315No Hit
ACACCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1030.15950692229070523No Hit
ACACCCGGGACCATAGGCATTAGCACTCTCTAAAGCAATGTGATTCATAAGAGTCCTA1010.1564097004986527No Hit
ACACCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCCAG1000.15486108960262646No Hit
ACACCCGGGGGGGTTGGAGAGATGGCTCAGAAGTTAAGAGCACTCACTGGTTGTTCTT980.1517638678105739No Hit
ACACCCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG980.1517638678105739No Hit
ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG980.1517638678105739No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA890.13782636974633752No Hit
ACACCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG880.13627775885031126No Hit
ACACCCGGGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAGGCATCTGCATCCATC870.134729147954285No Hit
ACACCCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA840.13008331526620623No Hit
ACACCCGGGCAGCAGACTCCATGGCTCAGACTTCCAATACAATATGAACTCAGTTGCA830.12853470437017994No Hit
ACACCCGGGGTAAATTGGGCGCCATCACACCTGGGCCATGGGAGGCTGGAGCTATGTT790.12234026078607489No Hit
ACACCCGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.1176944280979961No Hit
ACACCCGGGGGGCCCCACTCCTGTATTTCTATTTCAGAACAATCTGATCTAATGGTCC760.1176944280979961No Hit
ACACCCGGGCTGTTATGCTTCCTGACACTTTTCTTCCTTCTGGAGTTATTGACCTGTC750.11614581720196984No Hit
ACACCCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA730.1130485954099173No Hit
ACACCCGGGATGGTGTCTGGTGTTAAGGGGGATAAAGGGACGCCCCTGCCCCCAGCAG710.10995137361786476No Hit
ACACCCGGGATTGTTTATTACTTCTTGGGGATATAAGACATTTTTCTTCCTTTTCTAA700.1084027627218385No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA690.10685415182581225No Hit
ACACCCGGGGGTTCCGAGTCTCCTCGTTGCCACTAAGCAGCACGGCGTGGGACCCTAC690.10685415182581225No Hit
ACACCCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.10375693003375971No Hit
ACACCCGGGCAGCACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTGGTGGCC670.10375693003375971No Hit
ACACCCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.10375693003375971No Hit
ACACCCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.10220831913773346No Hit
ACACCCGGGGAAGTCTTTAGAACAAGCAGGTTGGTGGTGGCGCACACCTTTAGTCCCA660.10220831913773346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTG150.00715221452.0682752
TAGTTAT150.00715221452.0682750
AGTTATC150.00715221452.0682751
ATAACTT251.6250122E-552.0682751
TAAGCGT150.00715221452.0682751
ACAAGCG150.00715221452.0682751
GTTATCG150.00715221452.0682752
ATCCTAT150.007174204652.02791242
ATTAACA150.007174204652.02791244
TACATGG150.007174204652.02791245
GTTACTA150.007174204652.02791246
CTTATCT150.007174204652.02791243
TTAGTTA150.007174204652.02791249
CATAAGA150.007174204652.02791246
TACTAAG150.007174204652.02791248
TCATAAG150.007174204652.02791245
CAACTTG150.007174204652.02791249
TGAATTC150.007174204652.02791244
GTGACTT150.007174204652.02791249
ACCCCTG150.007174204652.02791241
Produced by FastQC (version 0.11.5)