FastQCFastQC Report
Tue 31 May 2016
SRR212900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64971
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12141.8685259577349895No Hit
ACACCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9521.4652691200689538No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4400.6772252235612811No Hit
ACACCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.6264333317941851No Hit
ACACCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2810.432500654138No Hit
ACACCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.39863939295993595No Hit
ACACCCGGGATTTAAAGTTTGTTCTCTTAAGAAATTCAACAGTTTGCTTCCTCTTCCC2180.33553431530990746No Hit
ACACCCGGGGGGGATATGCTTAGTAATGCTGTGTGATTTTCTGGAGGTTGATAACTTT1960.3016730541318435No Hit
ACACCCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.30013390589647687No Hit
ACACCCGGGAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCACT1680.2585769035415801No Hit
ACACCCGGGATCACTATGGTATCAAAGACTTGGCTACAGTTTTGTTCTACATGCTAGT1640.2524203106001139No Hit
ACACCCGGGGTCAAGTTCAGCATCTTTGTTTCATTCCTGTTGATATGTGTGACTTTCC1420.21855904942204984No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1400.21548075295131675No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1380.21240245648058367No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1290.1985501223622847No Hit
ACACCCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGC1280.19701097412691818No Hit
ACACCCGGGGGACCAATGCCCCAGTTGTCAGTCAAAGCCGTTGGTGTTTTCATTGTTT1270.1954718258915516No Hit
ACACCCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.18931523295008543No Hit
ACACCCGGGGTTTGGATGTATAAATAACTCAACTTCCCCAAATGTCAAGCTCTTTGTG1220.18777608471471888No Hit
ACACCCGGGATGGTTCAAGGTAAAACACTTTAAACAAGTTAAAAAATCAAAATGCTTC1210.18623693647935233No Hit
ACACCCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.18315864000861923No Hit
ACACCCGGGGCCCTGGCCCATCATAGTTTGTACTTCATGAAATATATTGTACATTTTA1180.18161949177325268No Hit
ACACCCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG1080.1662280094195872No Hit
ACACCCGGGAATTGTAAAATACTATGTACCTTCAATGAAATGCAAGTTACTAAGCGTG1030.15853226824275446No Hit
ACACCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC980.15083652706592174No Hit
ACACCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCCAG980.15083652706592174No Hit
ACACCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG970.1492973788305552No Hit
ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG960.14775823059518864No Hit
ACACCCGGGACCATAGGCATTAGCACTCTCTAAAGCAATGTGATTCATAAGAGTCCTA930.143140785889089No Hit
ACACCCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.1339058964768897No Hit
ACACCCGGGGGGGTTGGAGAGATGGCTCAGAAGTTAAGAGCACTCACTGGTTGTTCTT870.1339058964768897No Hit
ACACCCGGGATTGTTTATTACTTCTTGGGGATATAAGACATTTTTCTTCCTTTTCTAA860.13236674824152314No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA850.1308276000061566No Hit
ACACCCGGGGGGCCCCACTCCTGTATTTCTATTTCAGAACAATCTGATCTAATGGTCC840.12928845177079004No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA830.1277493035354235No Hit
ACACCCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA830.1277493035354235No Hit
ACACCCGGGCTGTTATGCTTCCTGACACTTTTCTTCCTTCTGGAGTTATTGACCTGTC810.12467100706469039No Hit
ACACCCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.12159271059395729No Hit
ACACCCGGGCAGCAGACTCCATGGCTCAGACTTCCAATACAATATGAACTCAGTTGCA780.12005356235859074No Hit
ACACCCGGGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAGGCATCTGCATCCATC770.1185144141232242No Hit
ACACCCGGGCGTGTGACACTGGGCTGTCCCATTTCACATAACTCAAAGGACAGGGCAA690.10620122824029184No Hit
ACACCCGGGCAGCACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTGGTGGCC690.10620122824029184No Hit
ACACCCGGGATGGTGTCTGGTGTTAAGGGGGATAAAGGGACGCCCCTGCCCCCAGCAG680.10466208000492529No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA660.10158378353419219No Hit
ACACCCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.10004463529882564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGT150.00714379652.08410621
TACCCCG150.00714379652.08410650
ACGCCGG307.7903314E-752.08410627
CTCAAAC150.00714379652.08410623
CACGCCG307.7903314E-752.08410626
AAGCGTG150.00714379652.08410652
TAAGTGG150.00714379652.08410623
CAATTCA150.00714379652.08410650
CTAAGCG150.00714379652.08410650
CAACACG150.00714379652.08410625
GCGCACG307.7903314E-752.08410623
GTCAGTC307.7903314E-752.08410627
CGCTCAA150.00714379652.08410621
AATTCAC150.00714379652.08410651
TAGTGTT150.00714379652.08410649
TAAGCGT150.00714379652.08410651
AAGTGGT150.00714379652.08410624
CCTTGTA150.00714379652.08410622
AACACGG150.00714379652.08410626
TCAAACT150.00714379652.08410624
Produced by FastQC (version 0.11.5)