SRR212899_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212899_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64801
Sequences flagged as poor quality	0
Sequence length	58
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACACCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1021	1.5755929692443018	No Hit
ACACCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	849	1.3101649665900217	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	406	0.6265335411490563	No Hit
ACACCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	379	0.5848675174765822	No Hit
ACACCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	280	0.4320920973441768	No Hit
ACACCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	248	0.38271014336198517	No Hit
ACACCCGGGATTTAAAGTTTGTTCTCTTAAGAAATTCAACAGTTTGCTTCCTCTTCCC	210	0.3240690730081326	No Hit
ACACCCGGGAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCACT	175	0.2700575608401105	No Hit
ACACCCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	170	0.26234163053039306	No Hit
ACACCCGGGGGGGATATGCTTAGTAATGCTGTGTGATTTTCTGGAGGTTGATAACTTT	165	0.2546257002206756	No Hit
ACACCCGGGATCACTATGGTATCAAAGACTTGGCTACAGTTTTGTTCTACATGCTAGT	141	0.21758923473403188	No Hit
ACACCCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	129	0.19907100199071004	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	126	0.19444144380487957	No Hit
ACACCCGGGGTCAAGTTCAGCATCTTTGTTTCATTCCTGTTGATATGTGTGACTTTCC	124	0.19135507168099258	No Hit
ACACCCGGGAATTGTAAAATACTATGTACCTTCAATGAAATGCAAGTTACTAAGCGTG	122	0.18826869955710562	No Hit
ACACCCGGGGCCCTGGCCCATCATAGTTTGTACTTCATGAAATATATTGTACATTTTA	113	0.1743800249996142	No Hit
ACACCCGGGGGACCAATGCCCCAGTTGTCAGTCAAAGCCGTTGGTGTTTTCATTGTTT	111	0.17129365287572723	No Hit
ACACCCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGC	110	0.16975046681378375	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	105	0.1620345365040663	No Hit
ACACCCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG	97	0.1496890480085184	No Hit
ACACCCGGGGTTTGGATGTATAAATAACTCAACTTCCCCAAATGTCAAGCTCTTTGTG	97	0.1496890480085184	No Hit
ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG	92	0.14197311769880094	No Hit
ACACCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	91	0.14042993163685744	No Hit
ACACCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCCAG	91	0.14042993163685744	No Hit
ACACCCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89	0.13734355951297048	No Hit
ACACCCGGGACCATAGGCATTAGCACTCTCTAAAGCAATGTGATTCATAAGAGTCCTA	88	0.135800373451027	No Hit
ACACCCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	84	0.12962762920325305	No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	82	0.12654125707936606	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	80	0.12345488495547909	No Hit
ACACCCGGGATGGTTCAAGGTAAAACACTTTAAACAAGTTAAAAAATCAAAATGCTTC	80	0.12345488495547909	No Hit
ACACCCGGGGGGCCCCACTCCTGTATTTCTATTTCAGAACAATCTGATCTAATGGTCC	78	0.1203685128315921	No Hit
ACACCCGGGGTAAATTGGGCGCCATCACACCTGGGCCATGGGAGGCTGGAGCTATGTT	78	0.1203685128315921	No Hit
ACACCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG	73	0.11265258252187466	No Hit
ACACCCGGGGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTG	70	0.1080230243360442	No Hit
ACACCCGGGGGGGTTGGAGAGATGGCTCAGAAGTTAAGAGCACTCACTGGTTGTTCTT	70	0.1080230243360442	No Hit
ACACCCGGGATGGTGTCTGGTGTTAAGGGGGATAAAGGGACGCCCCTGCCCCCAGCAG	70	0.1080230243360442	No Hit
ACACCCGGGCAGCAGACTCCATGGCTCAGACTTCCAATACAATATGAACTCAGTTGCA	69	0.10647983827410071	No Hit
ACACCCGGGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAGGCATCTGCATCCATC	67	0.10339346615021373	No Hit
ACACCCGGGCTGTTATGCTTCCTGACACTTTTCTTCCTTCTGGAGTTATTGACCTGTC	67	0.10339346615021373	No Hit
ACACCCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA	66	0.10185028008827025	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGT	15	0.006873981	52.592796	6
CGGGTGC	20	3.2367467E-4	52.592796	6
CGGGTAC	15	0.006873981	52.592796	6
AGACTAG	15	0.006873981	52.592796	10
CCGGGTT	20	3.2367467E-4	52.592796	5
CCGGGTG	105	0.0	52.592796	5
CCGGGTC	40	1.6698323E-9	52.592796	5
CCGGGCG	95	0.0	52.592796	5
CCGGGCC	40	1.6698323E-9	52.592796	5
GGAGCGC	15	0.006873981	52.592796	8
GGTAAAT	15	0.006873981	52.592796	9
GGAGATC	15	0.006873981	52.592796	8
GGGTCCA	15	0.006873981	52.592796	8
GATCAGA	15	0.006873981	52.592796	9
CGGGGTA	85	0.0	52.592796	6
GGGACGT	15	0.006873981	52.592796	7
GGGACGA	15	0.006873981	52.592796	8
GGGCGAC	20	3.2367467E-4	52.592796	7
CGGGCTA	15	0.006873981	52.592796	6
CGGGCGT	25	1.5312506E-5	52.592796	6

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content