FastQCFastQC Report
Tue 31 May 2016
SRR212898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63875
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11261.7628180039138943No Hit
ACACCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9371.46692759295499No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5270.8250489236790606No Hit
ACACCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4310.6747553816046967No Hit
ACACCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.48532289628180036No Hit
ACACCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2760.4320939334637965No Hit
ACACCCGGGATTTAAAGTTTGTTCTCTTAAGAAATTCAACAGTTTGCTTCCTCTTC2060.3225048923679061No Hit
ACACCCGGGGTTTGGATGTATAAATAACTCAACTTCCCCAAATGTCAAGCTCTTTG1930.3021526418786693No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1880.2943248532289628No Hit
ACACCCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.2771037181996086No Hit
ACACCCGGGAATTGTAAAATACTATGTACCTTCAATGAAATGCAAGTTACTAAGCG1690.2645792563600783No Hit
ACACCCGGGAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCA1670.2614481409001957No Hit
ACACCCGGGGGGGATATGCTTAGTAATGCTGTGTGATTTTCTGGAGGTTGATAACT1650.2583170254403131No Hit
ACACCCGGGATGGTTCAAGGTAAAACACTTTAAACAAGTTAAAAAATCAAAATGCT1510.23639921722113502No Hit
ACACCCGGGATCACTATGGTATCAAAGACTTGGCTACAGTTTTGTTCTACATGCTA1400.2191780821917808No Hit
ACACCCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.20195694716242663No Hit
ACACCCGGGGTCAAGTTCAGCATCTTTGTTTCATTCCTGTTGATATGTGTGACTTT1170.18317025440313112No Hit
ACACCCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.18160469667318982No Hit
ACACCCGGGGGACCAATGCCCCAGTTGTCAGTCAAAGCCGTTGGTGTTTTCATTGT1160.18160469667318982No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1150.18003913894324852No Hit
ACACCCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA1080.1690802348336595No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1060.1659491193737769No Hit
ACACCCGGGGCCCTGGCCCATCATAGTTTGTACTTCATGAAATATATTGTACATTT1020.15968688845401174No Hit
ACACCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCC1020.15968688845401174No Hit
ACACCCGGGGGAACGGATCGCTCTGGAGGAAGATGAGCGCCTAGAACGAGGCCTGG850.1330724070450098No Hit
ACACCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC830.1299412915851272No Hit
ACACCCGGGGGGGTTGGAGAGATGGCTCAGAAGTTAAGAGCACTCACTGGTTGTTC830.1299412915851272No Hit
ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA830.1299412915851272No Hit
ACACCCGGGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAGGCATCTGCATCCA820.12837573385518591No Hit
ACACCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA810.12681017612524462No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC800.12524461839530332No Hit
ACACCCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.12054794520547946No Hit
ACACCCGGGACCATAGGCATTAGCACTCTCTAAAGCAATGTGATTCATAAGAGTCC770.12054794520547946No Hit
ACACCCGGGCTGTTATGCTTCCTGACACTTTTCTTCCTTCTGGAGTTATTGACCTG710.1111545988258317No Hit
ACACCCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.10802348336594912No Hit
ACACCCGGGCAGCAGACTCCATGGCTCAGACTTCCAATACAATATGAACTCAGTTG690.10802348336594912No Hit
ACACCCGGGGTGCTGTTTGTGAATAACATGTTTAAACCATCTGCTAATGTGATTAT660.10332681017612523No Hit
ACACCCGGGGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTG640.10019569471624266No Hit
ACACCCGGGGAAGTCTTTAGAACAAGCAGGTTGGTGGTGGCGCACACCTTTAGTCC640.10019569471624266No Hit
ACACCCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG640.10019569471624266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTAT251.9927142E-550.3019849
ATGGATA204.0321535E-450.3019848
CTTAATC150.00819702850.3019749
CCTAGAC150.00819702850.3019749
GATGGAT204.0479298E-450.26224547
GTGCAAC150.008222750.2622447
AACCTCA150.008222750.2622447
CATAAGA204.063755E-450.22257646
TCTACAT150.00824843250.22257246
TCATAAG204.0796294E-450.18296445
TTCATAA204.0796294E-450.18296444
GTTCTAC150.00827422350.1829644
TTGTGCA150.00827422350.1829645
ATATGAC150.00827422350.1829642
TATGACC150.00827422350.1829643
CGCCTGG150.00827422350.1829644
GAAACCT150.00827422350.1829645
ATGACCT150.00827422350.1829644
CTTGTCA204.095553E-450.1434238
TCTTGTC204.095553E-450.1434237
Produced by FastQC (version 0.11.5)