##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212898_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 63875 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.029526418786695 39.0 38.0 40.0 35.0 40.0 2 38.07525636007828 39.0 38.0 40.0 35.0 40.0 3 36.84084540117417 39.0 36.0 40.0 31.0 40.0 4 37.993534246575344 39.0 38.0 40.0 35.0 40.0 5 38.06301369863014 39.0 38.0 40.0 35.0 40.0 6 38.230559686888455 39.0 38.0 40.0 35.0 40.0 7 38.205260273972605 39.0 38.0 40.0 35.0 40.0 8 38.236461839530335 39.0 38.0 40.0 35.0 40.0 9 38.20021917808219 39.0 38.0 40.0 35.0 40.0 10 37.13584344422701 39.0 36.0 40.0 33.0 40.0 11 36.84344422700587 39.0 36.0 40.0 32.0 40.0 12 36.919655577299416 39.0 36.0 40.0 32.0 40.0 13 36.909166340508804 39.0 36.0 40.0 32.0 40.0 14 36.83392563600783 39.0 36.0 40.0 31.0 40.0 15 36.92461839530333 39.0 36.0 40.0 32.0 40.0 16 36.79484931506849 39.0 36.0 40.0 32.0 40.0 17 36.526340508806264 38.0 35.0 40.0 31.0 40.0 18 36.51284540117417 38.0 35.0 40.0 31.0 40.0 19 36.47694716242661 38.0 35.0 40.0 31.0 40.0 20 36.330317025440316 38.0 35.0 40.0 30.0 40.0 21 36.3866771037182 38.0 35.0 40.0 31.0 40.0 22 36.279624266144815 38.0 35.0 40.0 30.0 40.0 23 36.031045009784734 38.0 35.0 40.0 30.0 40.0 24 35.90429745596869 38.0 35.0 40.0 29.0 40.0 25 35.90799217221135 38.0 35.0 40.0 30.0 40.0 26 35.43893542074364 38.0 35.0 39.0 29.0 40.0 27 35.29418395303327 38.0 35.0 39.0 28.0 40.0 28 34.96762426614482 38.0 34.0 39.0 27.0 40.0 29 35.00297455968689 38.0 34.0 39.0 28.0 40.0 30 34.721753424657535 38.0 34.0 39.0 27.0 40.0 31 34.23635225048924 38.0 33.0 39.0 26.0 40.0 32 33.965542074363995 38.0 33.0 39.0 24.0 40.0 33 33.87070058708415 38.0 33.0 39.0 24.0 40.0 34 34.015045009784735 38.0 33.0 39.0 25.0 40.0 35 33.68519765166341 38.0 33.0 39.0 23.0 40.0 36 33.75124853228963 38.0 33.0 39.0 23.0 40.0 37 33.70000782778865 38.0 33.0 39.0 23.0 40.0 38 33.38321722113503 38.0 33.0 39.0 22.0 40.0 39 33.29665753424658 38.0 33.0 39.0 21.0 40.0 40 33.019334637964775 37.0 33.0 39.0 19.0 40.0 41 32.69388649706458 37.0 32.0 39.0 15.0 40.0 42 32.59946771037182 37.0 32.0 39.0 14.0 40.0 43 32.382982387475536 36.0 32.0 39.0 12.0 40.0 44 32.1947084148728 36.0 32.0 39.0 8.0 40.0 45 31.886293542074363 36.0 31.0 39.0 2.0 40.0 46 31.276978473581213 36.0 31.0 39.0 2.0 40.0 47 31.009440313111547 36.0 30.0 39.0 2.0 40.0 48 30.72566731898239 35.0 30.0 39.0 2.0 40.0 49 30.588665362035226 35.0 30.0 39.0 2.0 40.0 50 30.232187866927593 35.0 30.0 39.0 2.0 40.0 51 29.58370254403131 35.0 29.0 39.0 2.0 40.0 52 29.377534246575344 35.0 29.0 39.0 2.0 40.0 53 29.143060665362036 35.0 28.0 39.0 2.0 40.0 54 28.80164383561644 35.0 28.0 39.0 2.0 40.0 55 28.41816046966732 35.0 27.0 39.0 2.0 40.0 56 16.053463796477494 20.0 2.0 23.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.1145197655554071 1 2 0.36040558200228645 1 3 0.3636262310950258 1 4 -0.3514445327798157 1 5 0.4098468413375045 1 6 0.26863873202644584 1 7 0.04870283762818417 1 8 -0.20010382085980183 1 9 -0.4097995045412759 1 10 0.03924309859401376 1 11 0.3419128970368064 1 12 0.20601026205410022 1 13 0.22970634163054626 1 14 0.5734248264806681 1 15 0.6686834598886762 1 16 0.2656826461427144 1 17 0.8969827195255178 1 18 0.6709573798270867 1 19 -0.8339472843379028 1 20 0.9875332090366342 1 21 -0.4241851177728506 1 22 0.48416369716956353 1 23 -0.05373454557519608 1 24 0.06337598373772835 1 25 0.52472184861724 1 26 0.8036444308299124 1 27 0.7125819162630407 1 28 0.29096272908319776 1 29 -0.3881318902017483 1 30 0.26455153103899676 1 31 -1.0771287531790676 1 32 0.010020681312141733 1 33 0.7302505116636979 1 34 0.30382314839501845 1 35 0.09861977140180045 1 36 0.2777168298393562 1 37 -0.10221658648969623 1 38 1.164685884723717 1 39 0.32462340203830564 1 40 0.4153121325099036 1 41 -0.5240603700255875 1 42 -0.3996205647051383 1 43 0.030486477965823156 1 44 -0.6959992711483203 1 45 -0.26795073492425203 1 46 -1.9968651415673158 1 47 -0.41206925747504286 1 48 -1.6243282899332314 1 49 -0.5063588122362681 1 50 -1.7988052997258812 1 51 -1.1367317816643059 1 52 -1.7152005530969028 1 53 -1.3698081516804734 1 54 -0.5422612232688735 1 55 -1.2042876451531193 1 56 -0.7307506230447878 2 1 -1.2665544032425515 2 2 -0.8586907521835556 2 3 -1.9454104270464896 2 4 -0.866789801322021 2 5 -0.2823952728994925 2 6 -0.9372436209147281 2 7 -0.6588845451510181 2 8 -0.7316468728632088 2 9 -0.6433885923503126 2 10 -0.4113110361033492 2 11 -0.725009524105559 2 12 -1.3971440431462412 2 13 -1.358102695027597 2 14 -0.30381302518173925 2 15 -0.6915040933935046 2 16 -1.1524759216322238 2 17 -0.5616703069024425 2 18 -0.762120199030548 2 19 -0.8411936611494966 2 20 -1.2469083936914132 2 21 -0.36749287565861266 2 22 -0.05036315705038419 2 23 0.6108008337939452 2 24 0.7692583366789023 2 25 -0.6594213738891526 2 26 0.21583539417176922 2 27 0.33747535189816347 2 28 -0.1246382939347086 2 29 -1.8770491962546032 2 30 -0.03638623537191421 2 31 -1.1969070993001267 2 32 -1.8335428310493214 2 33 -2.3362456520191692 2 34 -0.5934488038641419 2 35 -1.8434092141054457 2 36 -0.6528032042612395 2 37 -0.5826087433524378 2 38 -0.5706082329233411 2 39 0.5697214412539893 2 40 0.18726439167443942 2 41 0.38258924634270386 2 42 0.5986744054568405 2 43 0.9311684899010331 2 44 0.7711789044697497 2 45 -0.4780956624604862 2 46 -0.5846741782254554 2 47 -0.7922909113539873 2 48 -0.4653342575376662 2 49 -1.138072367223483 2 50 -1.183715785659384 2 51 -0.051054032303692765 2 52 -0.19985528455470458 2 53 -0.5492625421323076 2 54 0.32304141952737453 2 55 -0.09303444822217344 2 56 -0.5210319529680607 3 1 0.24978546603849594 3 2 0.37006741775108054 3 3 -1.0107699041706724 3 4 0.4142559503119685 3 5 0.29712542426825905 3 6 -0.2869168235291113 3 7 -0.4835032815344604 3 8 -0.1848059142575451 3 9 -0.19240820019344795 3 10 -0.5638164827264376 3 11 -0.10736246528202997 3 12 0.40568820086247825 3 13 0.2562763899397211 3 14 0.7626357765611829 3 15 -0.15337773173772717 3 16 0.5523171066902179 3 17 0.15946259070103963 3 18 0.9906031125162968 3 19 0.5847322647764344 3 20 0.540673305654991 3 21 0.8874090851257037 3 22 0.906063858200163 3 23 0.4986003980640987 3 24 0.9337463541081021 3 25 0.8242387568298 3 26 -0.0031188541942483994 3 27 0.38649495974129877 3 28 0.4962767387450313 3 29 -0.3454587823112547 3 30 0.33943075809213497 3 31 1.2900210052750367 3 32 1.7612284107807454 3 33 0.640878530987365 3 34 0.8923899760922822 3 35 1.8047389340427031 3 36 2.1827087783095678 3 37 2.2496352653621585 3 38 1.4980192180570526 3 39 2.065364142779046 3 40 2.774410361173345 3 41 0.7682101613753787 3 42 1.438543686502591 3 43 1.4692948515568105 3 44 2.128477379415287 3 45 1.7256080412432198 3 46 2.5111863882233436 3 47 2.276336539626403 3 48 2.89741084050155 3 49 2.190903667634906 3 50 3.3195521881968233 3 51 0.7964405210732153 3 52 1.278358223070569 3 53 1.4256829916367586 3 54 1.820862770289903 3 55 1.174939407986976 3 56 -0.17680537344736358 4 1 0.2517011748507585 4 2 -0.005411509451981544 4 3 -0.40923733712086374 4 4 0.2092751074000887 4 5 -0.2648158273281638 4 6 -0.5244647162494189 4 7 -0.013516052926540567 4 8 -0.23908433060492484 4 9 -0.10620130364172553 4 10 -1.2426159718707552 4 11 -1.751040626201572 4 12 -0.9806464096100669 4 13 -0.6728423840066355 4 14 -0.6364702259930937 4 15 -0.7466088939344075 4 16 -0.10987957274784321 4 17 -0.7709333224534944 4 18 -0.32229599348625726 4 19 -0.6457913622989224 4 20 -0.30032286292738064 4 21 -0.997648386138664 4 22 -0.27145849173598435 4 23 -0.8340943656651518 4 24 -0.7469688438484781 4 25 -1.1604355726721138 4 26 -0.564421536186579 4 27 -0.09626366094835959 4 28 -0.8447194298373404 4 29 -1.3371573774581194 4 30 -0.03604816911092712 4 31 0.24595970259304778 4 32 0.11946595867346588 4 33 0.09681722830537609 4 34 0.5999250974205097 4 35 0.43819998129685445 4 36 0.7139986889098253 4 37 0.828817896268923 4 38 0.9060651950685426 4 39 1.3418647813486473 4 40 1.1754320725398841 4 41 1.978938130724039 4 42 1.589884682671169 4 43 1.3760636893601301 4 44 1.4554250167077498 4 45 0.48167445248204643 4 46 0.5833447534851572 4 47 0.4889802177873257 4 48 1.035341874984308 4 49 0.05361120275623321 4 50 1.0130387782351384 4 51 1.4509743652622333 4 52 0.9654591170680149 4 53 0.8963087898487636 4 54 0.6458946987701069 4 55 0.3856673773356363 4 56 0.008379811737821541 5 1 -0.9367224704694408 5 2 -0.8779484552647929 5 3 -1.163547681948451 5 4 -0.665109129053107 5 5 -1.1724512953084556 5 6 -0.9277898394021236 5 7 -0.7758313238624979 5 8 -0.5611286655802985 5 9 -0.799551057336302 5 10 -1.263551932461887 5 11 -1.029981512901081 5 12 -0.3568779367036612 5 13 -1.501660117996785 5 14 -1.1494012604758552 5 15 -1.6838010121610125 5 16 -1.4423918880187827 5 17 -1.8669924357539855 5 18 -1.5642910673778871 5 19 -1.2042888992447303 5 20 -1.1894854245037365 5 21 -1.338781391064778 5 22 -1.4715816444453438 5 23 -1.5754736760099775 5 24 -0.633052587690841 5 25 -1.3285390209479786 5 26 -0.46343631451170353 5 27 -0.8277907545444165 5 28 -0.6981677056994116 5 29 0.3641973023448344 5 30 -0.4264422577808773 5 31 -0.3667250264709949 5 32 0.3128157123680424 5 33 -0.1230103579015207 5 34 -0.27692219321988887 5 35 -0.22436159505782882 5 36 0.6721267677275549 5 37 0.3084976992245885 5 38 1.1111144561522863 5 39 0.6103376877525903 5 40 0.4106537474167311 5 41 0.9844800026452205 5 42 0.9187024166613185 5 43 0.40315728541924045 5 44 0.8870752630131733 5 45 0.6349995756347511 5 46 1.0217683988053565 5 47 1.1096698729597385 5 48 1.14741084050155 5 49 0.9144486411798809 5 50 0.3572506008952381 5 51 2.001466976099671 5 52 1.5627497574621039 5 53 2.3390427799965465 5 54 1.6409685903957225 5 55 2.980494963542533 5 56 0.7583798117378215 6 1 -0.37984416359113027 6 2 -0.407710360026698 6 3 -0.7885476819484509 6 4 -1.1783366422806267 6 5 -0.46213383499100047 6 6 -0.26839830501059225 6 7 -0.24276254079371995 6 8 0.018897789446157276 6 9 -0.22944523723048604 6 10 -0.6378905568005138 6 11 0.059304201384634325 6 12 -0.3165340213597503 6 13 0.07109120475453778 6 14 0.003376517301923343 6 15 -0.30152587988587953 6 16 -0.07731252293941537 6 17 -1.3035003722619223 6 18 -1.5649524430392603 6 19 0.15880633885050344 6 20 -0.6074748424931542 6 21 -1.2422205445039296 6 22 -1.3717139195776156 6 23 -0.7236218241581227 6 24 -0.621809201447455 6 25 0.23164616423720474 6 26 0.6265107754353849 6 27 0.12723570048203925 6 28 0.4036841461524432 6 29 0.5619486250961572 6 30 1.2098011284625088 6 31 0.7529839682380057 6 32 1.094561744114074 6 33 1.7705081606169983 6 34 0.9664640501663584 6 35 1.4528870821908484 6 36 2.256782852383644 6 37 2.1385241542510443 6 38 1.9054266254644574 6 39 1.6949937724086794 6 40 1.1447807315437188 6 41 1.5459879391531572 6 42 1.975580723539629 6 43 1.8581837404456962 6 44 2.4247736757115845 6 45 1.373756189391365 6 46 1.677853054890015 6 47 1.2578180211078838 6 48 2.434447877538588 6 49 1.5612740380052799 6 50 1.7269595956042316 6 51 1.9816257062583986 6 52 2.093173037885382 6 53 2.166423732377499 6 54 2.783825733252865 6 55 2.860124593172163 6 56 0.11949092284893226 7 1 -0.3309273222214628 7 2 0.019962595948143758 7 3 0.3435277897496647 7 4 0.3429771243161639 7 5 -0.7189472521817706 7 6 0.22146892070568924 7 7 0.20867002384639477 7 8 -0.20297502676628199 7 9 0.052874818674474966 7 10 0.5039682831715311 7 11 0.4555306164789741 7 12 -0.021635349102581358 7 13 0.5434881299816539 7 14 -0.3970427699098167 7 15 -0.36372014962731924 7 16 -0.5919875753503163 7 17 0.0036275103376581797 7 18 -0.03699996225658708 7 19 0.26572457784421744 7 20 0.46415338951243257 7 21 -0.14403745575479832 7 22 0.2470841237487278 7 23 -0.6603793363873365 7 24 -0.219642884186797 7 25 -0.6705201530234532 7 26 0.38157716257724417 7 27 -0.5285993798813422 7 28 -1.155714875510732 7 29 -0.46565444967672676 7 30 0.1874391438363716 7 31 -0.6649056194629068 7 32 -0.3603648806238695 7 33 -0.08763299941095681 7 34 0.340328480634561 7 35 0.22577317583166234 7 36 -0.40604034817540935 7 37 -0.3380656430934721 7 38 -0.5051254360310011 7 39 -0.30745206965981 7 40 -0.9485106730684407 7 41 0.26716194334603216 7 42 -0.5226722464114602 7 43 0.10800903744080159 7 44 0.7937464220428225 7 45 0.49045779665901534 7 46 1.3357845713120966 7 47 1.147405722016341 7 48 0.6615617838977776 7 49 0.7143138702903933 7 50 0.9883152909221913 7 51 0.556049186342257 7 52 0.30735892188258873 7 53 -0.43420519844011096 7 54 -1.3765516252377026 7 55 -0.9487503194763356 7 56 0.008379811737821541 8 1 -0.1510081847551561 8 2 0.13990868759235298 8 3 0.40788088948011847 8 4 -0.004394843338822341 8 5 0.4704058475486832 8 6 -0.12421841083070007 8 7 -0.007974181005359071 8 8 -0.5075572370088679 8 9 -0.10312248590773265 8 10 0.7543052103952519 8 11 -1.1906957986153657 8 12 -0.8033065081322306 8 13 -0.3409458322825003 8 14 -0.2922584033330011 8 15 0.15548470212470278 8 16 0.2004652548383632 8 17 -0.7241352928968467 8 18 -0.29643392452074124 8 19 0.08142538646955444 8 20 -0.2251997102180212 8 21 0.2505043232209374 8 22 -0.09658164444534378 8 23 0.5852406097043072 8 24 0.06337598373772835 8 25 0.028603836194875498 8 26 0.6437065426311506 8 27 0.3150663883127294 8 28 0.9089751514434425 8 29 -0.08223126908373501 8 30 0.8057005993619768 8 31 0.15113211638615098 8 32 -0.1157557162033811 8 33 0.3055610706699099 8 34 0.06236352106582643 8 35 0.6863526906564559 8 36 0.6542696248704161 8 37 1.237069127796019 8 38 0.5396858847237169 8 39 1.1103376877525903 8 40 1.5713680331310158 8 41 1.9487657169309358 8 42 2.3115595595184644 8 43 1.5995858568478099 8 44 0.8870752630131733 8 45 -0.20428613865096068 8 46 -0.031803029766074076 8 47 1.1632413015311691 8 48 0.1652679833586923 8 49 0.9144486411798809 8 50 0.7143934580380957 8 51 1.251466976099671 8 52 0.38417832889067327 8 53 1.2318999228536889 8 54 1.8731114475385802 8 55 1.5162092492568178 8 56 1.1155226688806792 9 1 0.00833247458550801 9 2 -0.016684719001055726 9 3 -0.28854768194845093 9 4 0.21126449732051356 9 5 0.264361891504727 9 6 0.11479257818029254 9 7 0.29284999481881613 9 8 0.4127724333208036 9 9 0.49989949211424545 9 10 0.3051293862194271 9 11 -0.8637727216922855 9 12 0.015374810549083406 9 13 -0.6774842938209602 9 14 0.09098335490875797 9 15 -0.10138342974342862 9 16 -0.5110732067000967 9 17 -0.7351243038858541 9 18 -0.27720315528997475 9 19 -0.48862955858539436 9 20 -0.9614634464817584 9 21 -0.7536165558999386 9 22 0.17814363027993352 9 23 -0.19085829139459065 9 24 -0.39816247780073155 9 25 0.5272302098212549 9 26 -0.6997000507754407 9 27 0.04034111358745207 9 28 0.051832294300588444 9 29 -1.0327807196331875 9 30 -0.7973213786600013 9 31 -1.2142524989984622 9 32 -2.5635579140055818 9 33 -2.2287795886707507 9 34 -2.309889226186918 9 35 -3.3946912653874968 9 36 -2.7289721333713466 9 37 -2.679139663412773 9 38 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26 -1.0441816561266108 120 27 -0.26567895330217794 120 28 -0.861211183960279 120 29 -1.257697107593053 120 30 -0.061535425482745154 120 31 0.11852342073397892 120 32 0.010020681312141733 120 33 0.5345983377506514 120 34 0.7386057570906743 120 35 -0.5970324025112461 120 36 -0.5918483875519485 120 37 -0.2109122386636102 120 38 -0.6179228109284551 120 39 -1.349289641439956 120 40 -0.5194704761857523 120 41 -0.04579950046036885 120 42 -0.18222926035731746 120 43 -0.3608178698602629 120 44 0.08660942450385534 120 45 0.6885710042061817 120 46 1.3727000758239924 120 47 1.414017699046692 120 48 1.5278456231102453 120 49 1.3414672747202516 120 50 1.353368613317599 120 51 2.5806595226835185 120 52 3.1108864034248356 120 53 2.9345396744064836 120 54 3.8273039941224276 120 55 2.2087558331077517 120 56 3.030118942172603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45.0 3 5.0 4 10.0 5 12.0 6 31.0 7 65.0 8 49.0 9 95.0 10 119.0 11 126.0 12 155.0 13 218.0 14 221.0 15 295.0 16 333.0 17 436.0 18 423.0 19 445.0 20 505.0 21 557.0 22 532.0 23 618.0 24 720.0 25 758.0 26 885.0 27 1012.0 28 1184.0 29 1331.0 30 1577.0 31 1889.0 32 2277.0 33 2916.0 34 3697.0 35 5017.0 36 6874.0 37 9865.0 38 10630.0 39 7948.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 52.30058708414873 35.8371819960861 4.212915851272016 7.649315068493151 11 34.92915851272016 25.29628180039139 25.53424657534247 14.240313111545987 12 32.03913894324853 18.348336594911938 25.604696673189824 24.007827788649706 13 24.394520547945206 22.23091976516634 27.528767123287672 25.845792563600785 14 21.11624266144814 31.729158512720158 28.09549902152642 19.059099804305283 15 25.96634050880626 33.39804305283757 24.344422700587085 16.29119373776908 16 27.192172211350297 34.182387475538164 23.15146771037182 15.473972602739728 17 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20.70160582110149 30 21.021746634449432 33.25540527818747 25.39303982175919 20.329808265603916 31 24.104704811525064 33.65870499984307 24.176893380622076 18.059696808009793 32 22.86194281766655 33.54006060510904 26.14184107644722 17.45615550077719 33 22.76540262025197 32.250463413867855 25.283546451349398 19.700587514530774 34 20.629733211827922 34.09797944882632 28.381987870408192 16.89029946893756 35 18.43656194502507 37.258168445205804 28.17583177481966 16.129437834949474 36 19.785541335178138 36.519291846168365 25.48033080720732 18.21483601144618 37 23.175162391280413 35.44926943583775 24.145578081502336 17.2299900913795 38 19.529615354361678 37.611893337528514 24.453708802013686 18.404782506096122 39 21.604782882315924 34.779735682819386 26.538703587161738 17.07677784770296 40 19.908697363242815 35.357733175914994 26.005509641873275 18.72805981896891 41 17.537148973680896 36.39025311673593 26.007429794736183 20.065168114846994 42 18.811148047201172 34.78171821089282 25.391898917650025 21.015234824255984 43 20.040329568190053 34.55739874285174 25.651810892134158 19.750460796824047 44 20.5668730601377 34.817475697562664 24.789273841596952 19.826377400702683 45 20.30484056554703 34.50026007597371 25.971344356351374 19.223555002127895 46 21.068810593520926 34.78678962717743 25.09970836289115 19.0446914164105 47 18.923181251872762 34.955605671119244 25.97107666104181 20.150136415966188 48 18.220118343195267 34.16962524654832 28.635897435897434 18.974358974358974 49 21.79679245580559 34.18068850235756 25.106839291627896 18.915679750208948 50 19.60685324835137 33.17767330325309 27.25522986148361 19.960243586911936 51 19.040106023792244 35.40595121643369 25.972673629737148 19.58126913003692 52 18.610414003188282 36.002335969190455 26.519563741970103 18.86768628565115 53 18.84254728001768 34.909860133236506 27.4966059419695 18.750986644776308 54 17.913205520987965 34.695682385268945 27.326995357063893 20.064116736679193 55 19.94947102479078 34.20969524711827 25.07974103900205 20.7610926890889 56 19.24048634138639 35.3671245855045 25.201326385599245 20.19106268750987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 175.0 13 1030.5 14 1711.0 15 2170.5 16 2630.0 17 2175.0 18 1720.0 19 1343.0 20 966.0 21 818.5 22 566.0 23 461.0 24 490.5 25 520.0 26 531.5 27 543.0 28 683.0 29 926.5 30 1030.0 31 1180.5 32 1331.0 33 1509.5 34 1688.0 35 1587.0 36 1486.0 37 2007.5 38 2068.0 39 1607.0 40 1558.5 41 1510.0 42 1892.0 43 2274.0 44 2096.5 45 1919.0 46 2203.0 47 2433.0 48 2379.0 49 2312.0 50 2245.0 51 2253.0 52 2261.0 53 2540.5 54 3032.5 55 3245.0 56 3182.5 57 3120.0 58 3120.5 59 3121.0 60 3206.0 61 3291.0 62 3533.5 63 3476.5 64 3177.0 65 2615.5 66 2054.0 67 1701.0 68 1348.0 69 1152.5 70 957.0 71 794.5 72 516.0 73 400.0 74 349.0 75 298.0 76 243.0 77 188.0 78 152.5 79 96.0 80 75.0 81 62.0 82 49.0 83 46.5 84 44.0 85 26.5 86 9.0 87 9.0 88 6.0 89 3.0 90 1.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.05009784735812133 26 0.0547945205479452 27 0.1111545988258317 28 0.13776908023483367 29 0.1675146771037182 30 0.2207436399217221 31 0.23953033268101762 32 0.28806262230919766 33 0.33972602739726027 34 0.3585127201565558 35 0.38512720156555774 36 0.4273972602739726 37 0.46027397260273967 38 0.48532289628180036 39 0.4931506849315068 40 0.547945205479452 41 0.5432485322896282 42 0.6293542074363992 43 0.6230919765166341 44 0.6324853228962818 45 0.676320939334638 46 0.6888454011741683 47 0.7295499021526419 48 0.7827788649706457 49 0.7232876712328767 50 0.7655577299412916 51 0.7718199608610568 52 0.810958904109589 53 0.8281800391389432 54 0.8657534246575344 55 0.853228962818004 56 0.853228962818004 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 63875.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.046575342465754 #Duplication Level Percentage of deduplicated Percentage of total 1 62.124955877162016 19.28767123287671 2 11.603045736473199 7.204696673189824 3 6.217538197771166 5.790998043052838 4 4.039130654026525 5.016046966731898 5 3.0205234229237052 4.688845401174168 6 2.3347284554485404 4.349119373776908 7 1.9313196510513841 4.197260273972603 8 1.669103928193233 4.14559686888454 9 1.1749281428067166 3.2829745596868882 >10 5.54182845040593 28.042270058708414 >50 0.22187484241843577 4.328767123287671 >100 0.10589481115425345 5.610958904109589 >500 0.0100852201099289 2.291976516634051 >1k 0.00504261005496445 1.7628180039138943 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACACCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1126 1.7628180039138943 No Hit ACACCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 937 1.46692759295499 No Hit ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 527 0.8250489236790606 No Hit ACACCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 431 0.6747553816046967 No Hit ACACCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 310 0.48532289628180036 No Hit ACACCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 276 0.4320939334637965 No Hit ACACCCGGGATTTAAAGTTTGTTCTCTTAAGAAATTCAACAGTTTGCTTCCTCTTC 206 0.3225048923679061 No Hit ACACCCGGGGTTTGGATGTATAAATAACTCAACTTCCCCAAATGTCAAGCTCTTTG 193 0.3021526418786693 No Hit ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 188 0.2943248532289628 No Hit ACACCCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 177 0.2771037181996086 No Hit ACACCCGGGAATTGTAAAATACTATGTACCTTCAATGAAATGCAAGTTACTAAGCG 169 0.2645792563600783 No Hit ACACCCGGGAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCA 167 0.2614481409001957 No Hit ACACCCGGGGGGGATATGCTTAGTAATGCTGTGTGATTTTCTGGAGGTTGATAACT 165 0.2583170254403131 No Hit ACACCCGGGATGGTTCAAGGTAAAACACTTTAAACAAGTTAAAAAATCAAAATGCT 151 0.23639921722113502 No Hit ACACCCGGGATCACTATGGTATCAAAGACTTGGCTACAGTTTTGTTCTACATGCTA 140 0.2191780821917808 No Hit ACACCCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 129 0.20195694716242663 No Hit ACACCCGGGGTCAAGTTCAGCATCTTTGTTTCATTCCTGTTGATATGTGTGACTTT 117 0.18317025440313112 No Hit ACACCCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 116 0.18160469667318982 No Hit ACACCCGGGGGACCAATGCCCCAGTTGTCAGTCAAAGCCGTTGGTGTTTTCATTGT 116 0.18160469667318982 No Hit ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 115 0.18003913894324852 No Hit ACACCCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA 108 0.1690802348336595 No Hit ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 106 0.1659491193737769 No Hit ACACCCGGGGCCCTGGCCCATCATAGTTTGTACTTCATGAAATATATTGTACATTT 102 0.15968688845401174 No Hit ACACCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCC 102 0.15968688845401174 No Hit ACACCCGGGGGAACGGATCGCTCTGGAGGAAGATGAGCGCCTAGAACGAGGCCTGG 85 0.1330724070450098 No Hit ACACCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 83 0.1299412915851272 No Hit ACACCCGGGGGGGTTGGAGAGATGGCTCAGAAGTTAAGAGCACTCACTGGTTGTTC 83 0.1299412915851272 No Hit ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 83 0.1299412915851272 No Hit ACACCCGGGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAGGCATCTGCATCCA 82 0.12837573385518591 No Hit ACACCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 81 0.12681017612524462 No Hit ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 80 0.12524461839530332 No Hit ACACCCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.12054794520547946 No Hit ACACCCGGGACCATAGGCATTAGCACTCTCTAAAGCAATGTGATTCATAAGAGTCC 77 0.12054794520547946 No Hit ACACCCGGGCTGTTATGCTTCCTGACACTTTTCTTCCTTCTGGAGTTATTGACCTG 71 0.1111545988258317 No Hit ACACCCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.10802348336594912 No Hit ACACCCGGGCAGCAGACTCCATGGCTCAGACTTCCAATACAATATGAACTCAGTTG 69 0.10802348336594912 No Hit ACACCCGGGGTGCTGTTTGTGAATAACATGTTTAAACCATCTGCTAATGTGATTAT 66 0.10332681017612523 No Hit ACACCCGGGGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTG 64 0.10019569471624266 No Hit ACACCCGGGGAAGTCTTTAGAACAAGCAGGTTGGTGGTGGCGCACACCTTTAGTCC 64 0.10019569471624266 No Hit ACACCCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 64 0.10019569471624266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0015655577299412916 0.0 0.0 0.0 0.0 12 0.0015655577299412916 0.0 0.0 0.0 0.0 13 0.0015655577299412916 0.0 0.0 0.0 0.0 14 0.0031311154598825833 0.0 0.0 0.0 0.0 15 0.0031311154598825833 0.0 0.0 0.0 0.0 16 0.0031311154598825833 0.0 0.0 0.0 0.0 17 0.0031311154598825833 0.0 0.0 0.0 0.0 18 0.0031311154598825833 0.0 0.0 0.0 0.0 19 0.0031311154598825833 0.0 0.0 0.0 0.0 20 0.012524461839530333 0.0 0.0 0.0 0.0 21 0.014090019569471625 0.0 0.0 0.0 0.0 22 0.0187866927592955 0.0 0.0 0.0 0.0 23 0.025048923679060666 0.0 0.0 0.0 0.0 24 0.02974559686888454 0.0 0.0 0.0 0.0 25 0.02974559686888454 0.0 0.0 0.0 0.0 26 0.03131115459882583 0.0 0.0 0.0 0.0 27 0.03600782778864971 0.0 0.0 0.0 0.0 28 0.07984344422700587 0.0 0.0 0.0 0.0 29 0.08297455968688845 0.0 0.0 0.0 0.0 30 0.09393346379647749 0.0 0.0 0.0 0.0 31 0.09863013698630137 0.0 0.0 0.0 0.0 32 0.10019569471624266 0.0 0.0 0.0 0.0 33 0.10019569471624266 0.0 0.0 0.0 0.0 34 0.10176125244618395 0.0 0.0 0.0 0.0 35 0.14246575342465753 0.0 0.0 0.0 0.0 36 0.14403131115459883 0.0 0.0 0.0 0.0 37 0.14403131115459883 0.0 0.0 0.0 0.0 38 0.14403131115459883 0.0 0.0 0.0 0.0 39 0.14403131115459883 0.0 0.0 0.0 0.0 40 0.14559686888454013 0.0 0.0 0.0 0.0 41 0.1471624266144814 0.0 0.0 0.0 0.0 42 0.1487279843444227 0.0 0.0 0.0 0.0 43 0.1487279843444227 0.0 0.0 0.0 0.0 44 0.1518590998043053 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCTAT 25 1.9927142E-5 50.30198 49 ATGGATA 20 4.0321535E-4 50.30198 48 CTTAATC 15 0.008197028 50.30197 49 CCTAGAC 15 0.008197028 50.30197 49 GATGGAT 20 4.0479298E-4 50.262245 47 GTGCAAC 15 0.0082227 50.26224 47 AACCTCA 15 0.0082227 50.26224 47 CATAAGA 20 4.063755E-4 50.222576 46 TCTACAT 15 0.008248432 50.222572 46 TCATAAG 20 4.0796294E-4 50.182964 45 TTCATAA 20 4.0796294E-4 50.182964 44 GTTCTAC 15 0.008274223 50.18296 44 TTGTGCA 15 0.008274223 50.18296 45 ATATGAC 15 0.008274223 50.18296 42 TATGACC 15 0.008274223 50.18296 43 CGCCTGG 15 0.008274223 50.18296 44 GAAACCT 15 0.008274223 50.18296 45 ATGACCT 15 0.008274223 50.18296 44 CTTGTCA 20 4.095553E-4 50.14342 38 TCTTGTC 20 4.095553E-4 50.14342 37 >>END_MODULE