SRR212897_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212897_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58347
Sequences flagged as poor quality	0
Sequence length	56
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACACCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1072	1.8372838363583388	No Hit
ACACCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	858	1.470512622756954	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	456	0.7815311841225769	No Hit
ACACCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	350	0.5998594614975921	No Hit
ACACCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	241	0.41304608634548473	No Hit
ACACCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	231	0.39590724458841076	No Hit
ACACCCGGGGTTTGGATGTATAAATAACTCAACTTCCCCAAATGTCAAGCTCTTTG	174	0.2982158465730886	No Hit
ACACCCGGGAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCA	164	0.28107700481601455	No Hit
ACACCCGGGATTTAAAGTTTGTTCTCTTAAGAAATTCAACAGTTTGCTTCCTCTTC	154	0.26393816305894047	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	152	0.2605103947075257	No Hit
ACACCCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	145	0.24851320547757383	No Hit
ACACCCGGGAATTGTAAAATACTATGTACCTTCAATGAAATGCAAGTTACTAAGCG	141	0.2416576687747442	No Hit
ACACCCGGGGGGGATATGCTTAGTAATGCTGTGTGATTTTCTGGAGGTTGATAACT	141	0.2416576687747442	No Hit
ACACCCGGGATCACTATGGTATCAAAGACTTGGCTACAGTTTTGTTCTACATGCTA	126	0.21594940613913313	No Hit
ACACCCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114	0.19538279603064423	No Hit
ACACCCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114	0.19538279603064423	No Hit
ACACCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCC	113	0.19366891185493684	No Hit
ACACCCGGGATGGTTCAAGGTAAAACACTTTAAACAAGTTAAAAAATCAAAATGCT	111	0.19024114350352203	No Hit
ACACCCGGGGGACCAATGCCCCAGTTGTCAGTCAAAGCCGTTGGTGTTTTCATTGT	110	0.18852725932781464	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	107	0.1833856068006924	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	104	0.17824395427357018	No Hit
ACACCCGGGGTCAAGTTCAGCATCTTTGTTTCATTCCTGTTGATATGTGTGACTTT	96	0.16453288086791096	No Hit
ACACCCGGGGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTG	94	0.16110511251649615	No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	93	0.15939122834078873	No Hit
ACACCCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA	85	0.14568015493512948	No Hit
ACACCCGGGCTGTTATGCTTCCTGACACTTTTCTTCCTTCTGGAGTTATTGACCTG	81	0.13882461823229986	No Hit
ACACCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	79	0.13539684988088505	No Hit
ACACCCGGGGCCCTGGCCCATCATAGTTTGTACTTCATGAAATATATTGTACATTT	77	0.13196908152947023	No Hit
ACACCCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	77	0.13196908152947023	No Hit
ACACCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	75	0.12854131317805545	No Hit
ACACCCGGGGGGGTTGGAGAGATGGCTCAGAAGTTAAGAGCACTCACTGGTTGTTC	74	0.12682742900234803	No Hit
ACACCCGGGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAGGCATCTGCATCCA	73	0.1251135448266406	No Hit
ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	72	0.12339966065093322	No Hit
ACACCCGGGGGAACGGATCGCTCTGGAGGAAGATGAGCGCCTAGAACGAGGCCTGG	68	0.11654412394810358	No Hit
ACACCCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	68	0.11654412394810358	No Hit
ACACCCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67	0.11483023977239618	No Hit
ACACCCGGGAGTCCACATCTATTGTTACAATCTAGAAATTTCCTTTGTGCAACTGT	67	0.11483023977239618	No Hit
ACACCCGGGATGGTGTCTGGTGTTAAGGGGGATAAAGGGACGCCCCTGCCCCCAGC	63	0.10797470306956657	No Hit
ACACCCGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63	0.10797470306956657	No Hit
ACACCCGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.10283305054244435	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCACTT	20	4.0889264E-4	50.152584	49
CAGTCAC	15	0.008290504	50.152584	47
TCAAGCT	35	5.050788E-8	50.152584	45
TTCGTCA	15	0.008290504	50.152584	50
GTCAAGC	35	5.050788E-8	50.152584	44
TGTTCTA	25	2.0259751E-5	50.152584	43
CTAAGCG	20	4.0889264E-4	50.152584	50
AGCAGTC	15	0.008290504	50.152584	45
TTTAGCA	15	0.008290504	50.152584	42
CTTCGTC	15	0.008290504	50.152584	49
ACGGCGT	15	0.008290504	50.152584	42
GTTACTA	20	4.0889264E-4	50.152584	46
AGTCACT	20	4.0889264E-4	50.152584	48
CAGCCTT	15	0.008290504	50.152584	45
CCAAATG	35	5.0853487E-8	50.10939	38
CCCAAAT	35	5.0853487E-8	50.10939	37
CAGTTCC	15	0.008318824	50.109386	40
CAGCACG	15	0.008318824	50.109386	38
CCTGCGT	15	0.008318824	50.109386	40
CATCCAA	15	0.008318824	50.109386	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content