FastQCFastQC Report
Tue 31 May 2016
SRR212896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193109
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13780.7135866272416097No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10480.5426986831271458No Hit
ACGAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9410.4872895618536681No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8630.4468978659720676No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA7510.3888995334241283No Hit
ACGAGCGGGAGAGTCCTGTAGTCGTGGGTGGTCTTATGGTTGTGTGTTCTGTCACCAT6770.35057920656209707No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG6060.31381240646474273No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5680.29413440077883474No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA5620.29102734724948087No Hit
ACGAGCGGGGCTCCTCCCAGGTGACTGCACAGGATGTGCGGAAGGAAAGTCCATTGCT5510.2853310824456654No Hit
ACGAGCGGGAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGCAGTGGAATTCATC4680.24235017528960326No Hit
ACGAGCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG4400.2278505921526185No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4280.2216364850939107No Hit
ACGAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT3650.18901242303569488No Hit
ACGAGCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGACA3590.18590536950634098No Hit
ACGAGCGGGGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACC3470.1796912624476332No Hit
ACGAGCGGGATAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCA3240.16778089058510998No Hit
ACGAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3130.1620846257812945No Hit
ACGAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.15742404548726366No Hit
ACGAGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.15742404548726366No Hit
ACGAGCGGGACTCTTTTTGTCCACTGGAGAGTGAGAGAACTGTTCACAGTTCATCTGT2840.14706720038941737No Hit
ACGAGCGGGAGATATTTCAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAGGC2800.14499583136984812No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2630.13619251303667876No Hit
ACGAGCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2490.12894272146818636No Hit
ACGAGCGGGATTTGACTTAACTCCACAAGTTTGATTAAACTTGTGTAGAAAACCCCTT2320.120139403135017No Hit
ACGAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2240.11599666509587848No Hit
ACGAGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.1123717693116323No Hit
ACGAGCGGGAGCCAATTATGTGAATATGTCTTTCTCAGTTGTGATAGCCAGAGAGAGT2130.11030040029206305No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTACTCTTCCG2090.10822903127249378No Hit
ACGAGCGGGGTGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA2050.10615766225292453No Hit
ACGAGCGGGAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTGTT2020.10460413548824758No Hit
ACGAGCGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCG2010.10408629323335526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGC150.00717575152.0646150
TTTCGCA150.00717575152.0646151
TATCAGC150.007183118352.05110549
CGACGCT203.4282118E-452.037643
ATAGCTT150.007190491552.037635
CACGCAA150.007190491552.037633
TTCCGTA203.4282118E-452.037644
TAGCCGG150.007190491552.037637
GTAATAT150.007190491552.037633
CCGTAGT150.007190491552.037646
CGTAGTA150.007190491552.037647
ACTCGGA150.007190491552.037648
ACCCGGT307.9206256E-752.037644
TCGGTTG203.4282118E-452.037637
ATACAAC307.9206256E-752.037634
CGCCAAC203.4282118E-452.037645
AACGTTA150.007197870452.02411330
ACGTTAC150.007197870452.02411331
TTGCTAC150.007197870452.02411331
AGTGGGC150.007197870452.02411331