SRR212896_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212896_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	193109
Sequences flagged as poor quality	0
Sequence length	58
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACGAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1378	0.7135866272416097	No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	1048	0.5426986831271458	No Hit
ACGAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	941	0.4872895618536681	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	863	0.4468978659720676	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA	751	0.3888995334241283	No Hit
ACGAGCGGGAGAGTCCTGTAGTCGTGGGTGGTCTTATGGTTGTGTGTTCTGTCACCAT	677	0.35057920656209707	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	606	0.31381240646474273	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	568	0.29413440077883474	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA	562	0.29102734724948087	No Hit
ACGAGCGGGGCTCCTCCCAGGTGACTGCACAGGATGTGCGGAAGGAAAGTCCATTGCT	551	0.2853310824456654	No Hit
ACGAGCGGGAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGCAGTGGAATTCATC	468	0.24235017528960326	No Hit
ACGAGCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG	440	0.2278505921526185	No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	428	0.2216364850939107	No Hit
ACGAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT	365	0.18901242303569488	No Hit
ACGAGCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGACA	359	0.18590536950634098	No Hit
ACGAGCGGGGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACC	347	0.1796912624476332	No Hit
ACGAGCGGGATAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCA	324	0.16778089058510998	No Hit
ACGAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	313	0.1620846257812945	No Hit
ACGAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	304	0.15742404548726366	No Hit
ACGAGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	304	0.15742404548726366	No Hit
ACGAGCGGGACTCTTTTTGTCCACTGGAGAGTGAGAGAACTGTTCACAGTTCATCTGT	284	0.14706720038941737	No Hit
ACGAGCGGGAGATATTTCAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAGGC	280	0.14499583136984812	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	263	0.13619251303667876	No Hit
ACGAGCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC	249	0.12894272146818636	No Hit
ACGAGCGGGATTTGACTTAACTCCACAAGTTTGATTAAACTTGTGTAGAAAACCCCTT	232	0.120139403135017	No Hit
ACGAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	224	0.11599666509587848	No Hit
ACGAGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	217	0.1123717693116323	No Hit
ACGAGCGGGAGCCAATTATGTGAATATGTCTTTCTCAGTTGTGATAGCCAGAGAGAGT	213	0.11030040029206305	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTACTCTTCCG	209	0.10822903127249378	No Hit
ACGAGCGGGGTGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA	205	0.10615766225292453	No Hit
ACGAGCGGGAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTGTT	202	0.10460413548824758	No Hit
ACGAGCGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCG	201	0.10408629323335526	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTCGC	15	0.007175751	52.06461	50
TTTCGCA	15	0.007175751	52.06461	51
TATCAGC	15	0.0071831183	52.051105	49
CGACGCT	20	3.4282118E-4	52.0376	43
ATAGCTT	15	0.0071904915	52.0376	35
CACGCAA	15	0.0071904915	52.0376	33
TTCCGTA	20	3.4282118E-4	52.0376	44
TAGCCGG	15	0.0071904915	52.0376	37
GTAATAT	15	0.0071904915	52.0376	33
CCGTAGT	15	0.0071904915	52.0376	46
CGTAGTA	15	0.0071904915	52.0376	47
ACTCGGA	15	0.0071904915	52.0376	48
ACCCGGT	30	7.9206256E-7	52.0376	44
TCGGTTG	20	3.4282118E-4	52.0376	37
ATACAAC	30	7.9206256E-7	52.0376	34
CGCCAAC	20	3.4282118E-4	52.0376	45
AACGTTA	15	0.0071978704	52.024113	30
ACGTTAC	15	0.0071978704	52.024113	31
TTGCTAC	15	0.0071978704	52.024113	31
AGTGGGC	15	0.0071978704	52.024113	31

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content