FastQCFastQC Report
Tue 31 May 2016
SRR212895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192900
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12940.6708138932089165No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10250.5313634007257646No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA9030.4681181959564541No Hit
ACGAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8900.46137895282529806No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA7240.37532400207361327No Hit
ACGAGCGGGAGAGTCCTGTAGTCGTGGGTGGTCTTATGGTTGTGTGTTCTGTCACCAT6910.35821669258683253No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5630.2918610679108346No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA5380.2789009849663038No Hit
ACGAGCGGGGCTCCTCCCAGGTGACTGCACAGGATGTGCGGAAGGAAAGTCCATTGCT5220.270606531881804No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5190.2690513219284603No Hit
ACGAGCGGGAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGCAGTGGAATTCATC4350.2255054432348367No Hit
ACGAGCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG4320.22395023328149302No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4160.21565578019699327No Hit
ACGAGCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGACA3490.1809227579056506No Hit
ACGAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT3440.17833074131674442No Hit
ACGAGCGGGGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACC3160.16381544841886989No Hit
ACGAGCGGGATAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCA3120.16174183514774493No Hit
ACGAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.16174183514774493No Hit
ACGAGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.1565578019699326No Hit
ACGAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.15603939865215138No Hit
ACGAGCGGGAGATATTTCAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAGGC2840.1472265422498704No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTACTCTTCCG2670.13841368584758942No Hit
ACGAGCGGGACTCTTTTTGTCCACTGGAGAGTGAGAGAACTGTTCACAGTTCATCTGT2560.13271124935199585No Hit
ACGAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2340.12130637636080871No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2320.12026956972524624No Hit
ACGAGCGGGATTTGACTTAACTCCACAAGTTTGATTAAACTTGTGTAGAAAACCCCTT2220.1150855365474339No Hit
ACGAGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.11197511664074651No Hit
ACGAGCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2140.11093831000518403No Hit
ACGAGCGGGGTGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA2090.10834629341627787No Hit
ACGAGCGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCG2080.10782789009849664No Hit
ACGAGCGGGAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTGTT2050.10627268014515293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGATT150.007115136452.1763527
ATTGACA203.3834088E-452.1763527
CGTCTGA150.007129810352.1491824
CATTCGA150.007129810352.1491825
ATCACCG150.007129810352.1491825
CACCGCC150.007129810352.1491826
TGCCCGA150.007151863552.10848222
CCATTAC150.007151863552.10848223
GGCGTCA203.405228E-452.1084823
CGGCATA150.00716659452.08138351
GGCATAT150.00716659452.08138352
ACTAGTA150.007181346452.05431450
CTTAACG150.00719612252.02727539
TTTAACG150.00719612252.02727546
TAGGTAG203.4315587E-452.02727541
TTCCGTA203.4315587E-452.02727544
CGATTGT307.931412E-752.02727544
TAACGGC150.00719612252.02727548
TAACGAT150.00719612252.02727541
ACGATAT150.00719612252.02727543
Produced by FastQC (version 0.11.5)