FastQCFastQC Report
Tue 31 May 2016
SRR212894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191992
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11840.6166923621817576No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC9690.5047085295220635No Hit
ACGAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8400.4375182299262469No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8160.42501770907121134No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA6340.33022209258719115No Hit
ACGAGCGGGAGAGTCCTGTAGTCGTGGGTGGTCTTATGGTTGTGTGTTCTGTCACCAT5970.31095045626901124No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5190.2703237634901454No Hit
ACGAGCGGGGCTCCTCCCAGGTGACTGCACAGGATGTGCGGAAGGAAAGTCCATTGCT5020.26146922788449517No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA4900.25521896745697736No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4620.2406350264594358No Hit
ACGAGCGGGAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGCAGTGGAATTCATC4270.22240510021250887No Hit
ACGAGCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG3720.19375807325305222No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3510.18282011750489605No Hit
ACGAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT3300.17188216175673987No Hit
ACGAGCGGGGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACC3280.1708404516854869No Hit
ACGAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.15886078586607777No Hit
ACGAGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.1578190757948248No Hit
ACGAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.15469394558106586No Hit
ACGAGCGGGATAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCA2920.15208967040293345No Hit
ACGAGCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGACA2810.14636026501104213No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTACTCTTCCG2380.12396349847910329No Hit
ACGAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2360.12292178840785033No Hit
ACGAGCGGGACTCTTTTTGTCCACTGGAGAGTGAGAGAACTGTTCACAGTTCATCTGT2240.1166715279803325No Hit
ACGAGCGGGAGATATTTCAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAGGC2140.11146297762406768No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2140.11146297762406768No Hit
ACGAGCGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCG2100.10937955748156172No Hit
ACGAGCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2000.1041710071252969No Hit
ACGAGCGGGATTTGACTTAACTCCACAAGTTTGATTAAACTTGTGTAGAAAACCCCTT1930.1005250218759115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG203.3278274E-452.35147510
CGGGCGA203.3278274E-452.3514756
CGGGTCG150.007021387652.351476
CGGGTAT251.585454E-552.351476
GGGTCGT150.007021387652.351478
GGATCGA150.007021387652.351478
ACCACGA150.007021387652.3514710
GAACCAC251.585454E-552.351479
TCGGCGT150.007183395352.05044639
CGGCTGA150.007183395352.05044639
CACGTCC150.007183395352.05044638
ACTAGAC203.4239708E-452.0504439
CGTGGCG150.0071982652.0232527
ACTATGT150.0071982652.0232528
AACCGGT150.0071982652.0232528
ACCGGTA150.0071982652.0232529
AAATCGA203.4328192E-452.0232525
AATCACG150.0071982652.0232529
CCACTAT150.0071982652.0232526
CTTATCC150.0071982652.0232528
Produced by FastQC (version 0.11.5)