FastQCFastQC Report
Tue 31 May 2016
SRR212893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195610
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13170.6732784622463064No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12900.6594754869382956No Hit
ACGAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT9930.5076427585501764No Hit
ACGAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9010.4606103982413987No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8020.4099994887786923No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC7830.40028628393231436No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT7770.3972189560860897No Hit
ACGAGCGGGAGAGTCCTGTAGTCGTGGGTGGTCTTATGGTTGTGTGTTCTGTCACC5940.3036654567762384No Hit
ACGAGCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC5320.2719697356985839No Hit
ACGAGCGGGGCTCCTCCCAGGTGACTGCACAGGATGTGCGGAAGGAAAGTCCATTG4580.23413935892848012No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4300.2198251623127652No Hit
ACGAGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3580.18301722815806962No Hit
ACGAGCGGGAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGCAGTGGAATTCA3560.18199478554266144No Hit
ACGAGCGGGATTTGACTTAACTCCACAAGTTTGATTAAACTTGTGTAGAAAACCCC3260.16665814631153827No Hit
ACGAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.16461326108072183No Hit
ACGAGCGGGATAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATT3180.16256837584990544No Hit
ACGAGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.15490005623434386No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2950.15081028577271102No Hit
ACGAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2900.1482541792341905No Hit
ACGAGCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC2890.14774295792648637No Hit
ACGAGCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGA2500.12780532692602628No Hit
ACGAGCGGGGCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT2480.12678288431061807No Hit
ACGAGCGGGAGATATTTCAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAG2430.12422677777209754No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTACTCTTC2390.12218189254128112No Hit
ACGAGCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGT2360.1206482286181688No Hit
ACGAGCGGGAAGGAAGGTGACAAGCACACCCTGAGCAAGAAGGAGCTGAAGGAGTT2220.11349113031031133No Hit
ACGAGCGGGGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGCC2160.1104238024640867No Hit
ACGAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2090.10684525331015797No Hit
ACGAGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2080.10633403200245387No Hit
ACGAGCGGGAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTG2080.10633403200245387No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2040.10428914677163743No Hit
ACGAGCGGGGGCCATCTTCCACAAGTACTCTGGCAAGGAAGGTGACAAGCACACCC1970.1007105976177087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTA204.0998132E-450.1851450
AACCGAG301.017097E-650.18513549
CTCCGTA150.00829842750.18513550
ACCGAGT252.0360241E-550.18513550
TACATCG150.00829842750.18513549
ATATCTA150.00829842750.18513549
TCCCCTA150.00829842750.18513549
ATAACCG150.008306866550.17223448
CGTAGTA150.00831531450.1593447
TTCCGTA150.00832376650.14645444
TTGTACC150.00832376650.14645444
CCGTAGT150.00832376650.14645446
TCCGTAG150.00832376650.14645445
CGTACAC150.00832376650.14645444
CAATGCG150.00832376650.14645445
ACGTACA150.00832376650.14645443
ACCCGGT150.00832376650.14645444
ACATATC150.00832376650.14645441
ACCGCTC204.1154484E-450.1464546
TCTACCC150.0083406950.12069739
Produced by FastQC (version 0.11.5)